Decorin binds fibrinogen in a Zn2+-dependent interaction

Tracey A. Dugan, Vivian W C Yang, David J. McQuillan, Magnus Höök

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

We have previously shown that decorin, a member of the small leucine-rich proteoglycan family of extracellular matrix proteoglycans/glycoproteins is a Zn2+ metalloprotein at physiological Zn2+ concentrations (Yang, V. W-C., LaBrenz, S. R., Rosenberg, L. C., McQuillan, D., and Höök, M. (1999) J. Biol. Chem. 274, 12454-12460). We now report that the decorin proteoglycan binds fibrinogen in the presence of Zn2+. The fibrinogen-binding site is located in the N-terminal domain of the decorin core protein and a 45-amino acid peptide representing this domain binds to the fibrinogen D fragment with an apparent KD of 1.7 × 10-6 M, as determined from fluorescence polarization data. Furthermore, we show that Zn2+ promotes the self-association of decorin. The N-terminal domain of the core protein also mediates this activity. The results of solid-phase binding assays and gel filtration chromatography suggest that the N-terminal domain of decorin, when present at low micromolar concentrations, forms an oligomer in a Zn2+-dependent manner. Thus, Zn2+ appears to play a pivotal role in the interactions and biological function of decorin.

Original languageEnglish
Pages (from-to)13655-13662
Number of pages8
JournalJournal of Biological Chemistry
Volume278
Issue number16
DOIs
Publication statusPublished - Apr 18 2003

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Decorin
Fibrinogen
Proteoglycans
Metalloproteins
Fluorescence Polarization
Chromatography
Oligomers
Leucine
Gel Chromatography
Extracellular Matrix
Assays
Glycoproteins
Proteins
Gels
Fluorescence
Binding Sites
Association reactions
Polarization
Amino Acids
Peptides

ASJC Scopus subject areas

  • Biochemistry

Cite this

Decorin binds fibrinogen in a Zn2+-dependent interaction. / Dugan, Tracey A.; Yang, Vivian W C; McQuillan, David J.; Höök, Magnus.

In: Journal of Biological Chemistry, Vol. 278, No. 16, 18.04.2003, p. 13655-13662.

Research output: Contribution to journalArticle

Dugan, Tracey A. ; Yang, Vivian W C ; McQuillan, David J. ; Höök, Magnus. / Decorin binds fibrinogen in a Zn2+-dependent interaction. In: Journal of Biological Chemistry. 2003 ; Vol. 278, No. 16. pp. 13655-13662.
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