Deciphering the gene expression profile of peroxisome proliferator-activated receptor signaling pathway in the left atria of patients with mitral regurgitation

Mien Cheng Chen, Jen Ping Chang, Yu Sheng Lin, Kuo Li Pan, Wan Chun Ho, Wen Hao Liu, Tzu Hao Chang, Yao Kuang Huang, Chih Yuan Fang, Chien Jen Chen

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

Background: Differentially exprenked to peroxisome proliferator-activated receptor (PPAR) signaling pathway in the KEGssed genes in the left atria of mitral regurgitation (MR) pigs have been liG pathway. However, specific genes of the PPAR signaling pathway in the left atria of MR patients have never been explored. Methods: This study enrolled 15 MR patients with heart failure, 7 patients with aortic valve disease and heart failure, and 6 normal controls. We used PCR assay (84 genes) for PPAR pathway and quantitative RT-PCR to study specific genes of the PPAR pathway in the left atria. Results: Gene expression profiling analysis through PCR assay identified 23 genes to be differentially expressed in the left atria of MR patients compared to normal controls. The expressions of APOA1, ACADM, FABP3, ETFDH, ECH1, CPT1B, CPT2, SLC27A6, ACAA2, SMARCD3, SORBS1, EHHADH, SLC27A1, PPARGC1B, PPARA and CPT1A were significantly up-regulated, whereas the expression of PLTP was significantly down-regulated in the MR patients compared to normal controls. The expressions of HMGCS2, ACADM, FABP3, MLYCD, ECH1, ACAA2, EHHADH, CPT1A and PLTP were significantly up-regulated in the MR patients compared to patients with aortic valve disease. Notably, only ACADM, FABP3, ECH1, ACAA2, EHHADH, CPT1A and PLTP of the PPAR pathway were significantly differentially expressed in the MR patients compared to patients with aortic valve disease and normal controls. Conclusions: Differentially expressed genes of the PPAR pathway have been identified in the left atria of MR patients compared with patients with aortic valve disease and normal controls.

Original languageEnglish
Article number157
JournalJournal of Translational Medicine
Volume14
Issue number1
DOIs
Publication statusPublished - Jun 2 2016

Keywords

  • Atrium
  • Genes
  • Mitral regurgitation

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

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