DDX3 as a strongest prognosis marker and its downregulation promotes metastasis in colorectal cancer

Chia Yi Su, Tsung Chieh Lin, Yuan Feng Lin, Ming Huang Chen, Chien Hsin Lee, Hsuan Yao Wang, Yu Chieh Lee, Yu Peng Liu, Chi Long Chen, Michael Hsiao

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Background: Conflicting results regarding the role of DEAD-box polypeptide 3 (DDX3) are seen not only between cancer types but also within the same type of cancer. In this study, we aimed at clarifying the prognostic significance of DDX3 in patients of major cancer types through large cohort survival analysis and further investigated its effects on cancer progression. Methods: Large cohort survival analysis of 7 cancer types, including colorectal cancer, breast cancer, lung cancer, head and neck cancer, liver cancer, glioblastoma, and ovarian cancer, was performed using public database at RNA level and was further confirmed by IHC analysis at protein level. Phenotype parameters of DDX3 knockdown colon cancer cells and the mechanism of DDX3 regulated cancer progression were investigated in vitro and in vivo. Results: In large cohort survival analysis, DDX3 had a significant prognostic predictive power in colorectal cancer at both RNA and protein level. Patients with low DDX3 expression had poor prognosis and frequent distant metastasis. Knockdown of DDX3 enhanced the migration and invasion abilities of colon cancer cells and promoted tumor metastasis in vivo. Snail upregulation with decreased membranous E-cadherin expression and reduced cell aggregation were found after DDX3 downregulation. Conclusions: Our study revealed the strong prognostic effect of DDX3 on colorectal cancer among seven major cancer types through larger cohort survival analysis at RNA and protein level. Low DDX3 expression promotes Snail/E-cadherin pathway mediated cancer metastasis and poor clinical outcome in colorectal cancer patients.

Original languageEnglish
Pages (from-to)18602-18612
Number of pages11
JournalOncotarget
Volume6
Issue number21
Publication statusPublished - 2015

Fingerprint

Colorectal Neoplasms
Down-Regulation
Neoplasm Metastasis
Survival Analysis
Neoplasms
Cohort Studies
Head and Neck Neoplasms
Cadherins
Liver Neoplasms
Colonic Neoplasms
Lung Neoplasms
RNA
Breast Neoplasms
Cell Aggregation
Proteins
Nucleic Acid Databases
Snails
Glioblastoma
Ovarian Neoplasms
Up-Regulation

Keywords

  • Colorectal cancer
  • DDX3
  • E-cadherin
  • Metastasis
  • Prognosis

ASJC Scopus subject areas

  • Oncology

Cite this

Su, C. Y., Lin, T. C., Lin, Y. F., Chen, M. H., Lee, C. H., Wang, H. Y., ... Hsiao, M. (2015). DDX3 as a strongest prognosis marker and its downregulation promotes metastasis in colorectal cancer. Oncotarget, 6(21), 18602-18612.

DDX3 as a strongest prognosis marker and its downregulation promotes metastasis in colorectal cancer. / Su, Chia Yi; Lin, Tsung Chieh; Lin, Yuan Feng; Chen, Ming Huang; Lee, Chien Hsin; Wang, Hsuan Yao; Lee, Yu Chieh; Liu, Yu Peng; Chen, Chi Long; Hsiao, Michael.

In: Oncotarget, Vol. 6, No. 21, 2015, p. 18602-18612.

Research output: Contribution to journalArticle

Su, CY, Lin, TC, Lin, YF, Chen, MH, Lee, CH, Wang, HY, Lee, YC, Liu, YP, Chen, CL & Hsiao, M 2015, 'DDX3 as a strongest prognosis marker and its downregulation promotes metastasis in colorectal cancer', Oncotarget, vol. 6, no. 21, pp. 18602-18612.
Su, Chia Yi ; Lin, Tsung Chieh ; Lin, Yuan Feng ; Chen, Ming Huang ; Lee, Chien Hsin ; Wang, Hsuan Yao ; Lee, Yu Chieh ; Liu, Yu Peng ; Chen, Chi Long ; Hsiao, Michael. / DDX3 as a strongest prognosis marker and its downregulation promotes metastasis in colorectal cancer. In: Oncotarget. 2015 ; Vol. 6, No. 21. pp. 18602-18612.
@article{1c5e2a0587b14ce880a1f92177da390f,
title = "DDX3 as a strongest prognosis marker and its downregulation promotes metastasis in colorectal cancer",
abstract = "Background: Conflicting results regarding the role of DEAD-box polypeptide 3 (DDX3) are seen not only between cancer types but also within the same type of cancer. In this study, we aimed at clarifying the prognostic significance of DDX3 in patients of major cancer types through large cohort survival analysis and further investigated its effects on cancer progression. Methods: Large cohort survival analysis of 7 cancer types, including colorectal cancer, breast cancer, lung cancer, head and neck cancer, liver cancer, glioblastoma, and ovarian cancer, was performed using public database at RNA level and was further confirmed by IHC analysis at protein level. Phenotype parameters of DDX3 knockdown colon cancer cells and the mechanism of DDX3 regulated cancer progression were investigated in vitro and in vivo. Results: In large cohort survival analysis, DDX3 had a significant prognostic predictive power in colorectal cancer at both RNA and protein level. Patients with low DDX3 expression had poor prognosis and frequent distant metastasis. Knockdown of DDX3 enhanced the migration and invasion abilities of colon cancer cells and promoted tumor metastasis in vivo. Snail upregulation with decreased membranous E-cadherin expression and reduced cell aggregation were found after DDX3 downregulation. Conclusions: Our study revealed the strong prognostic effect of DDX3 on colorectal cancer among seven major cancer types through larger cohort survival analysis at RNA and protein level. Low DDX3 expression promotes Snail/E-cadherin pathway mediated cancer metastasis and poor clinical outcome in colorectal cancer patients.",
keywords = "Colorectal cancer, DDX3, E-cadherin, Metastasis, Prognosis",
author = "Su, {Chia Yi} and Lin, {Tsung Chieh} and Lin, {Yuan Feng} and Chen, {Ming Huang} and Lee, {Chien Hsin} and Wang, {Hsuan Yao} and Lee, {Yu Chieh} and Liu, {Yu Peng} and Chen, {Chi Long} and Michael Hsiao",
year = "2015",
language = "English",
volume = "6",
pages = "18602--18612",
journal = "Oncotarget",
issn = "1949-2553",
publisher = "Impact Journals LLC",
number = "21",

}

TY - JOUR

T1 - DDX3 as a strongest prognosis marker and its downregulation promotes metastasis in colorectal cancer

AU - Su, Chia Yi

AU - Lin, Tsung Chieh

AU - Lin, Yuan Feng

AU - Chen, Ming Huang

AU - Lee, Chien Hsin

AU - Wang, Hsuan Yao

AU - Lee, Yu Chieh

AU - Liu, Yu Peng

AU - Chen, Chi Long

AU - Hsiao, Michael

PY - 2015

Y1 - 2015

N2 - Background: Conflicting results regarding the role of DEAD-box polypeptide 3 (DDX3) are seen not only between cancer types but also within the same type of cancer. In this study, we aimed at clarifying the prognostic significance of DDX3 in patients of major cancer types through large cohort survival analysis and further investigated its effects on cancer progression. Methods: Large cohort survival analysis of 7 cancer types, including colorectal cancer, breast cancer, lung cancer, head and neck cancer, liver cancer, glioblastoma, and ovarian cancer, was performed using public database at RNA level and was further confirmed by IHC analysis at protein level. Phenotype parameters of DDX3 knockdown colon cancer cells and the mechanism of DDX3 regulated cancer progression were investigated in vitro and in vivo. Results: In large cohort survival analysis, DDX3 had a significant prognostic predictive power in colorectal cancer at both RNA and protein level. Patients with low DDX3 expression had poor prognosis and frequent distant metastasis. Knockdown of DDX3 enhanced the migration and invasion abilities of colon cancer cells and promoted tumor metastasis in vivo. Snail upregulation with decreased membranous E-cadherin expression and reduced cell aggregation were found after DDX3 downregulation. Conclusions: Our study revealed the strong prognostic effect of DDX3 on colorectal cancer among seven major cancer types through larger cohort survival analysis at RNA and protein level. Low DDX3 expression promotes Snail/E-cadherin pathway mediated cancer metastasis and poor clinical outcome in colorectal cancer patients.

AB - Background: Conflicting results regarding the role of DEAD-box polypeptide 3 (DDX3) are seen not only between cancer types but also within the same type of cancer. In this study, we aimed at clarifying the prognostic significance of DDX3 in patients of major cancer types through large cohort survival analysis and further investigated its effects on cancer progression. Methods: Large cohort survival analysis of 7 cancer types, including colorectal cancer, breast cancer, lung cancer, head and neck cancer, liver cancer, glioblastoma, and ovarian cancer, was performed using public database at RNA level and was further confirmed by IHC analysis at protein level. Phenotype parameters of DDX3 knockdown colon cancer cells and the mechanism of DDX3 regulated cancer progression were investigated in vitro and in vivo. Results: In large cohort survival analysis, DDX3 had a significant prognostic predictive power in colorectal cancer at both RNA and protein level. Patients with low DDX3 expression had poor prognosis and frequent distant metastasis. Knockdown of DDX3 enhanced the migration and invasion abilities of colon cancer cells and promoted tumor metastasis in vivo. Snail upregulation with decreased membranous E-cadherin expression and reduced cell aggregation were found after DDX3 downregulation. Conclusions: Our study revealed the strong prognostic effect of DDX3 on colorectal cancer among seven major cancer types through larger cohort survival analysis at RNA and protein level. Low DDX3 expression promotes Snail/E-cadherin pathway mediated cancer metastasis and poor clinical outcome in colorectal cancer patients.

KW - Colorectal cancer

KW - DDX3

KW - E-cadherin

KW - Metastasis

KW - Prognosis

UR - http://www.scopus.com/inward/record.url?scp=84938802096&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84938802096&partnerID=8YFLogxK

M3 - Article

C2 - 26087195

AN - SCOPUS:84938802096

VL - 6

SP - 18602

EP - 18612

JO - Oncotarget

JF - Oncotarget

SN - 1949-2553

IS - 21

ER -