DC-SIGN mediates avian H5N1 influenza virus infection in cis and in trans

Sheng Fan Wang, Jason C. Huang, Yuan Ming Lee, Shih Jen Liu, Yu Jiun Chan, Yat Pang Chau, Pele Chong, Yi Ming Arthur Chen

Research output: Contribution to journalArticlepeer-review

60 Citations (Scopus)

Abstract

DC-SIGN, a C-type lectin receptor expressed in dendritic cells (DCs), has been identified as a receptor for human immunodeficiency virus type 1, hepatitis C virus, Ebola virus, cytomegalovirus, dengue virus, and the SARS coronavirus. We used H5N1 pseudotyped and reverse-genetics (RG) virus particles to study their ability to bind with DC-SIGN. Electronic microscopy and functional assay results indicate that pseudotyped viruses containing both HA and NA proteins express hemagglutination and are capable of infecting cells expressing α-2,3-linked sialic acid receptors. Results from a capture assay show that DC-SIGN-expressing cells (including B-THP-1/DC-SIGN and T-THP-1/DC-SIGN) and peripheral blood dendritic cells are capable of transferring H5N1 pseudotyped and RG virus particles to target cells; this action can be blocked by anti-DC-SIGN monoclonal antibodies. In summary, (a) DC-SIGN acts as a capture or attachment molecule for avian H5N1 virus, and (b) DC-SIGN mediates infections in cis and in trans.

Original languageEnglish
Pages (from-to)561-566
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume373
Issue number4
DOIs
Publication statusPublished - Sep 5 2008
Externally publishedYes

Keywords

  • DC-SIGN
  • Dendritic cells
  • H5N1
  • Pseudotyped virus

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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