Daptomycin-loaded polymethylmethacrylate bone cement for joint arthroplasty surgery

Yuan Ming Hsu, Chun Hsing Liao, Yu Hong Wei, Hsu Wei Fang, Hsiang Huan Hou, Chia Chun Chen, Chih Hung Chang

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Antibiotic-loaded acrylic bone cement has been frequently used as an infection prophylaxis or antibiotic-loaded spacer in infected arthroplasty. In addition, daptomycin has been used recently against broad spectrum Gram-positive organisms. The goal of this in vitro study is to investigate the bacteriacidal and mechanical properties of daptomycin-incorporated polymethylmethacrylate (PMMA) bone cement and evaluate its feasibility for clinical use. Daptomycin (0.5, 1, or 2g) was premixed with 40g of PMMA bone cement powder before curing. The mechanical properties of the daptomycin-loaded acrylic bone cement (DLABC) were estimated following standard guidance, and the release profile and kinetics of daptomycin from PMMA were analyzed. The antimicrobial efficacy of DLABC was determined with a zone of inhibition (ZOI) assay against Staphylococcus aureus, Staphylococcus epidermis, Enterococcus faecalis, and Enterococcus faecium, respectively. The results showed that the compressive strength, of PMMA bone cement, which was higher than 100 MPa in all groups, was sufficient according to ISO 5833 after incorporation of daptomycin. The encapsulated daptomycin was released for 2 weeks with a 9.59±0.85%, 15.25±0.69%, and 20.64±20.33% released percentage on the first day in the low, mid, and high groups, respectively. According to the calculated release kinetics, incorporated daptomycin should be 3.3 times the original dose to double its release. Although all recipes of DLABC had a microbial inhibitory effect, the effect with a higher encapsulated amount of daptomycin was more significant. Therefore, we believe that daptomycin can be locally delivered from PMMA bone cement at the surgical site as a prophylactic or treatment for osteomyelitis against Gram-positive organisms with intact cement function.

Original languageEnglish
Pages (from-to)484-492
Number of pages9
JournalArtificial Organs
Volume38
Issue number6
DOIs
Publication statusPublished - Jan 1 2014
Externally publishedYes

Keywords

  • Acrylic bone cement
  • Antimicrobial efficacy
  • Daptomycin
  • Mechanical properties
  • Release kinetics

ASJC Scopus subject areas

  • Bioengineering
  • Medicine (miscellaneous)
  • Biomaterials
  • Biomedical Engineering

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  • Cite this

    Hsu, Y. M., Liao, C. H., Wei, Y. H., Fang, H. W., Hou, H. H., Chen, C. C., & Chang, C. H. (2014). Daptomycin-loaded polymethylmethacrylate bone cement for joint arthroplasty surgery. Artificial Organs, 38(6), 484-492. https://doi.org/10.1111/aor.12197