Daptomycin-loaded polymethylmethacrylate bone cement for joint arthroplasty surgery

Yuan Ming Hsu, Chun Hsing Liao, Yu Hong Wei, Hsu Wei Fang, Hsiang Huan Hou, Chia Chun Chen, Chih Hung Chang

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Antibiotic-loaded acrylic bone cement has been frequently used as an infection prophylaxis or antibiotic-loaded spacer in infected arthroplasty. In addition, daptomycin has been used recently against broad spectrum Gram-positive organisms. The goal of this in vitro study is to investigate the bacteriacidal and mechanical properties of daptomycin-incorporated polymethylmethacrylate (PMMA) bone cement and evaluate its feasibility for clinical use. Daptomycin (0.5, 1, or 2g) was premixed with 40g of PMMA bone cement powder before curing. The mechanical properties of the daptomycin-loaded acrylic bone cement (DLABC) were estimated following standard guidance, and the release profile and kinetics of daptomycin from PMMA were analyzed. The antimicrobial efficacy of DLABC was determined with a zone of inhibition (ZOI) assay against Staphylococcus aureus, Staphylococcus epidermis, Enterococcus faecalis, and Enterococcus faecium, respectively. The results showed that the compressive strength, of PMMA bone cement, which was higher than 100 MPa in all groups, was sufficient according to ISO 5833 after incorporation of daptomycin. The encapsulated daptomycin was released for 2 weeks with a 9.59±0.85%, 15.25±0.69%, and 20.64±20.33% released percentage on the first day in the low, mid, and high groups, respectively. According to the calculated release kinetics, incorporated daptomycin should be 3.3 times the original dose to double its release. Although all recipes of DLABC had a microbial inhibitory effect, the effect with a higher encapsulated amount of daptomycin was more significant. Therefore, we believe that daptomycin can be locally delivered from PMMA bone cement at the surgical site as a prophylactic or treatment for osteomyelitis against Gram-positive organisms with intact cement function.

Original languageEnglish
Pages (from-to)484-492
Number of pages9
JournalArtificial Organs
Volume38
Issue number6
DOIs
Publication statusPublished - Jan 1 2014
Externally publishedYes

Fingerprint

Daptomycin
Arthroplasty
Bone cement
Bone Cements
Polymethyl Methacrylate
Surgery
Joints
Acrylics
Antibiotics
Mechanical properties
Kinetics
Compressive strength
Anti-Bacterial Agents
Compressive Strength
Curing
Assays
Enterococcus faecium
Cements
Antibiotic Prophylaxis
Enterococcus faecalis

Keywords

  • Acrylic bone cement
  • Antimicrobial efficacy
  • Daptomycin
  • Mechanical properties
  • Release kinetics

ASJC Scopus subject areas

  • Bioengineering
  • Medicine (miscellaneous)
  • Biomaterials
  • Biomedical Engineering

Cite this

Hsu, Y. M., Liao, C. H., Wei, Y. H., Fang, H. W., Hou, H. H., Chen, C. C., & Chang, C. H. (2014). Daptomycin-loaded polymethylmethacrylate bone cement for joint arthroplasty surgery. Artificial Organs, 38(6), 484-492. https://doi.org/10.1111/aor.12197

Daptomycin-loaded polymethylmethacrylate bone cement for joint arthroplasty surgery. / Hsu, Yuan Ming; Liao, Chun Hsing; Wei, Yu Hong; Fang, Hsu Wei; Hou, Hsiang Huan; Chen, Chia Chun; Chang, Chih Hung.

In: Artificial Organs, Vol. 38, No. 6, 01.01.2014, p. 484-492.

Research output: Contribution to journalArticle

Hsu, YM, Liao, CH, Wei, YH, Fang, HW, Hou, HH, Chen, CC & Chang, CH 2014, 'Daptomycin-loaded polymethylmethacrylate bone cement for joint arthroplasty surgery', Artificial Organs, vol. 38, no. 6, pp. 484-492. https://doi.org/10.1111/aor.12197
Hsu, Yuan Ming ; Liao, Chun Hsing ; Wei, Yu Hong ; Fang, Hsu Wei ; Hou, Hsiang Huan ; Chen, Chia Chun ; Chang, Chih Hung. / Daptomycin-loaded polymethylmethacrylate bone cement for joint arthroplasty surgery. In: Artificial Organs. 2014 ; Vol. 38, No. 6. pp. 484-492.
@article{5613c3195c7d4942954feedba86bd864,
title = "Daptomycin-loaded polymethylmethacrylate bone cement for joint arthroplasty surgery",
abstract = "Antibiotic-loaded acrylic bone cement has been frequently used as an infection prophylaxis or antibiotic-loaded spacer in infected arthroplasty. In addition, daptomycin has been used recently against broad spectrum Gram-positive organisms. The goal of this in vitro study is to investigate the bacteriacidal and mechanical properties of daptomycin-incorporated polymethylmethacrylate (PMMA) bone cement and evaluate its feasibility for clinical use. Daptomycin (0.5, 1, or 2g) was premixed with 40g of PMMA bone cement powder before curing. The mechanical properties of the daptomycin-loaded acrylic bone cement (DLABC) were estimated following standard guidance, and the release profile and kinetics of daptomycin from PMMA were analyzed. The antimicrobial efficacy of DLABC was determined with a zone of inhibition (ZOI) assay against Staphylococcus aureus, Staphylococcus epidermis, Enterococcus faecalis, and Enterococcus faecium, respectively. The results showed that the compressive strength, of PMMA bone cement, which was higher than 100 MPa in all groups, was sufficient according to ISO 5833 after incorporation of daptomycin. The encapsulated daptomycin was released for 2 weeks with a 9.59±0.85{\%}, 15.25±0.69{\%}, and 20.64±20.33{\%} released percentage on the first day in the low, mid, and high groups, respectively. According to the calculated release kinetics, incorporated daptomycin should be 3.3 times the original dose to double its release. Although all recipes of DLABC had a microbial inhibitory effect, the effect with a higher encapsulated amount of daptomycin was more significant. Therefore, we believe that daptomycin can be locally delivered from PMMA bone cement at the surgical site as a prophylactic or treatment for osteomyelitis against Gram-positive organisms with intact cement function.",
keywords = "Acrylic bone cement, Antimicrobial efficacy, Daptomycin, Mechanical properties, Release kinetics",
author = "Hsu, {Yuan Ming} and Liao, {Chun Hsing} and Wei, {Yu Hong} and Fang, {Hsu Wei} and Hou, {Hsiang Huan} and Chen, {Chia Chun} and Chang, {Chih Hung}",
year = "2014",
month = "1",
day = "1",
doi = "10.1111/aor.12197",
language = "English",
volume = "38",
pages = "484--492",
journal = "Artificial Organs",
issn = "0160-564X",
publisher = "Wiley-Blackwell",
number = "6",

}

TY - JOUR

T1 - Daptomycin-loaded polymethylmethacrylate bone cement for joint arthroplasty surgery

AU - Hsu, Yuan Ming

AU - Liao, Chun Hsing

AU - Wei, Yu Hong

AU - Fang, Hsu Wei

AU - Hou, Hsiang Huan

AU - Chen, Chia Chun

AU - Chang, Chih Hung

PY - 2014/1/1

Y1 - 2014/1/1

N2 - Antibiotic-loaded acrylic bone cement has been frequently used as an infection prophylaxis or antibiotic-loaded spacer in infected arthroplasty. In addition, daptomycin has been used recently against broad spectrum Gram-positive organisms. The goal of this in vitro study is to investigate the bacteriacidal and mechanical properties of daptomycin-incorporated polymethylmethacrylate (PMMA) bone cement and evaluate its feasibility for clinical use. Daptomycin (0.5, 1, or 2g) was premixed with 40g of PMMA bone cement powder before curing. The mechanical properties of the daptomycin-loaded acrylic bone cement (DLABC) were estimated following standard guidance, and the release profile and kinetics of daptomycin from PMMA were analyzed. The antimicrobial efficacy of DLABC was determined with a zone of inhibition (ZOI) assay against Staphylococcus aureus, Staphylococcus epidermis, Enterococcus faecalis, and Enterococcus faecium, respectively. The results showed that the compressive strength, of PMMA bone cement, which was higher than 100 MPa in all groups, was sufficient according to ISO 5833 after incorporation of daptomycin. The encapsulated daptomycin was released for 2 weeks with a 9.59±0.85%, 15.25±0.69%, and 20.64±20.33% released percentage on the first day in the low, mid, and high groups, respectively. According to the calculated release kinetics, incorporated daptomycin should be 3.3 times the original dose to double its release. Although all recipes of DLABC had a microbial inhibitory effect, the effect with a higher encapsulated amount of daptomycin was more significant. Therefore, we believe that daptomycin can be locally delivered from PMMA bone cement at the surgical site as a prophylactic or treatment for osteomyelitis against Gram-positive organisms with intact cement function.

AB - Antibiotic-loaded acrylic bone cement has been frequently used as an infection prophylaxis or antibiotic-loaded spacer in infected arthroplasty. In addition, daptomycin has been used recently against broad spectrum Gram-positive organisms. The goal of this in vitro study is to investigate the bacteriacidal and mechanical properties of daptomycin-incorporated polymethylmethacrylate (PMMA) bone cement and evaluate its feasibility for clinical use. Daptomycin (0.5, 1, or 2g) was premixed with 40g of PMMA bone cement powder before curing. The mechanical properties of the daptomycin-loaded acrylic bone cement (DLABC) were estimated following standard guidance, and the release profile and kinetics of daptomycin from PMMA were analyzed. The antimicrobial efficacy of DLABC was determined with a zone of inhibition (ZOI) assay against Staphylococcus aureus, Staphylococcus epidermis, Enterococcus faecalis, and Enterococcus faecium, respectively. The results showed that the compressive strength, of PMMA bone cement, which was higher than 100 MPa in all groups, was sufficient according to ISO 5833 after incorporation of daptomycin. The encapsulated daptomycin was released for 2 weeks with a 9.59±0.85%, 15.25±0.69%, and 20.64±20.33% released percentage on the first day in the low, mid, and high groups, respectively. According to the calculated release kinetics, incorporated daptomycin should be 3.3 times the original dose to double its release. Although all recipes of DLABC had a microbial inhibitory effect, the effect with a higher encapsulated amount of daptomycin was more significant. Therefore, we believe that daptomycin can be locally delivered from PMMA bone cement at the surgical site as a prophylactic or treatment for osteomyelitis against Gram-positive organisms with intact cement function.

KW - Acrylic bone cement

KW - Antimicrobial efficacy

KW - Daptomycin

KW - Mechanical properties

KW - Release kinetics

UR - http://www.scopus.com/inward/record.url?scp=84901924290&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84901924290&partnerID=8YFLogxK

U2 - 10.1111/aor.12197

DO - 10.1111/aor.12197

M3 - Article

VL - 38

SP - 484

EP - 492

JO - Artificial Organs

JF - Artificial Organs

SN - 0160-564X

IS - 6

ER -