D-aspartate in a prolactin-secreting clonal strain of rat pituitary tumor cells (GH3)

Zhiqung Long, Jen Ai Lee, Taizo Okamoto, Noriyuki Nimura, Kazuhiro Imai, Hiroshi Homma

Research output: Contribution to journalArticlepeer-review

48 Citations (Scopus)


D-Aspartate (D-Asp) is found in prolactin (PRL)-containing cells of the rat anterior pituitary gland [Lee et al., Brain Res. 838, 193-199, 1999]. In order to determine whether D-Asp is actually produced by the anterior pituitary gland and whether it plays a physiological role in PRL function, a PRL-secreting clonal strain of rat pituitary tumor cells (GH3) was employed in this study. HPLC analysis and immunocytochemical staining detected the presence and synthesis of D-Asp in the cytoplasm of these cells. In addition, thyrotropin-releasing hormone-stimulated PRL secretion was increased in a dose-dependent fashion by D-Asp from these cells. These results suggest that the anterior pituitary gland synthesizes D-Asp and that D-Asp acts as a messenger in this gland. (C) 2000 Academic Press.

Original languageEnglish
Pages (from-to)1143-1147
Number of pages5
JournalBiochemical and Biophysical Research Communications
Issue number3
Publication statusPublished - Oct 5 2000


  • D-aspartate
  • GH cells
  • Pituitary gland
  • Prolactin
  • Thyrotropin-releasing hormone

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology


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