Cytotoxicity, oxidative stress, apoptosis and the autophagic effects of silver nanoparticles in mouse embryonic fibroblasts

Yu Hsuan Lee, Fong Yu Cheng, Hui Wen Chiu, Jui Chen Tsai, Chun Yong Fang, Chun Wan Chen, Ying Jan Wang

Research output: Contribution to journalArticle

167 Citations (Scopus)

Abstract

With the advancement of nanotechnology, nanomaterials have been comprehensively applied in our modern society. However, the hazardous impacts of nanoscale particles on organisms have not yet been thoroughly clarified. Currently, there exist numerous approaches to perform toxicity tests, but common and reasonable bio-indicators for toxicity evaluations are lacking. In this study, we investigated the effects of silver nanoparticles (AgNPs) on NIH 3T3 cells to explore the potential application of these nanoparticles in consumer products. Our results demonstrated that AgNPs were taken up by NIH 3T3 cells and localized within the intracellular endosomal compartments. Exposure to AgNPs is a potential source of oxidative stress, which leads to the induction of reactive oxygen species (ROS), the up-regulation of Heme oxygenase 1 (HO-1) expression, apoptosis and autophagy. Interestingly, AgNPs induced morphological and biochemical markers of autophagy in NIH 3T3 cells and induced autophagosome formation, as evidenced by transmission electron microscopic analysis, the formation of microtubule-associated protein-1 light chain-3 (LC3) puncta and the expression of LC3-II protein. Thus, autophagy activation may be a key player in the cellular response against nano-toxicity.

Original languageEnglish
Pages (from-to)4706-4715
Number of pages10
JournalBiomaterials
Volume35
Issue number16
DOIs
Publication statusPublished - 2014
Externally publishedYes

Fingerprint

NIH 3T3 Cells
Oxidative stress
Autophagy
Cell death
Fibroblasts
Cytotoxicity
Silver
Nanoparticles
Toxicity
Oxidative Stress
Apoptosis
Proteins
Light
Toxicity Tests
Heme Oxygenase-1
Nanotechnology
Microtubule-Associated Proteins
Consumer products
Nanostructures
Nanostructured materials

Keywords

  • Apoptosis
  • Autophagy
  • Cellular uptake
  • Cytotoxicity
  • Nanoparticle
  • ROS

ASJC Scopus subject areas

  • Biomaterials
  • Bioengineering
  • Ceramics and Composites
  • Mechanics of Materials
  • Biophysics

Cite this

Cytotoxicity, oxidative stress, apoptosis and the autophagic effects of silver nanoparticles in mouse embryonic fibroblasts. / Lee, Yu Hsuan; Cheng, Fong Yu; Chiu, Hui Wen; Tsai, Jui Chen; Fang, Chun Yong; Chen, Chun Wan; Wang, Ying Jan.

In: Biomaterials, Vol. 35, No. 16, 2014, p. 4706-4715.

Research output: Contribution to journalArticle

Lee, Yu Hsuan ; Cheng, Fong Yu ; Chiu, Hui Wen ; Tsai, Jui Chen ; Fang, Chun Yong ; Chen, Chun Wan ; Wang, Ying Jan. / Cytotoxicity, oxidative stress, apoptosis and the autophagic effects of silver nanoparticles in mouse embryonic fibroblasts. In: Biomaterials. 2014 ; Vol. 35, No. 16. pp. 4706-4715.
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