10 Citations (Scopus)

Abstract

Vitis thunbergii var. taiwaniana (VTT) is an indigenous Taiwanese wild grape and is used as a folk medicine in Taiwan. VTT is rich in polyphenols, especially quercetin and resveratrol derivatives, which were demonstrated to exhibit inhibitory activities against carcinogenesis and prevent some neurodegenerative diseases. (-)-Vitisin B is one of the resveratrol tetramers extracted from VTT. In this study, we investigated the mechanisms of (-)-vitisin B on the induction of apoptosis in human HL-60 promyelocytic leukemia cells. First, (-)-vitisin B significantly inhibited cell proliferation through inducing cell apoptosis. This effect appeared to occur in a time- and dose-dependent manner. Cell-cycle distribution was also examined, and we found that (-)-vitisin B significantly induced a sub-G1 population in a dose-dependent manner. In addition, (-)-vitisin B exhibited stronger inhibitory effects on cell proliferation than resveratrol. Second, (-)-vitisin B dose dependently induced apoptosis-related protein expressions, such as the cleavage form of caspase-3, caspase-8, caspase-9, poly(ADP ribose) polymerase, and the proapoptotic Bax protein. Third, (-)-vitisin B treatment also resulted in increases in c-Jun N-terminal kinase (JNK) phosphorylation and Fas ligand (FasL) expression. Moreover, the (-)-vitisin B-induced FasL expression and caspase-3 activation could be reversed by a JNK inhibitor. These results suggest that (-)-vitisin B-induced apoptosis of leukemia cells might be mediated through activation of JNK and Fas death-signal transduction.

Original languageEnglish
Pages (from-to)313-319
Number of pages7
JournalDrug and Chemical Toxicology
Volume36
Issue number3
DOIs
Publication statusPublished - Jul 2013

Fingerprint

Resveratrol
Cell death
Cytotoxicity
Leukemia
Cell proliferation
Chemical activation
Ligands
Neurodegenerative diseases
Proteins
Administrative data processing
Signal transduction
Apoptosis
Phosphorylation
Fas Ligand Protein
Vitis
Medicine
Caspase 3
Cells
Phosphotransferases
Derivatives

Keywords

  • (-)-vitisin B
  • Apoptosis
  • Fas ligand
  • JNK
  • Leukemia
  • Vitis thunbergii var. taiwaniana

ASJC Scopus subject areas

  • Toxicology
  • Pharmacology
  • Health, Toxicology and Mutagenesis
  • Public Health, Environmental and Occupational Health
  • Chemical Health and Safety

Cite this

Cytotoxicity of (-)-vitisin B in human leukemia cells. / Wu, Shing Sheng; Chen, Lih Geeng; Lin, Ren-Jye; Lin, Shyr Yi; Lo, Yueh E.; Liang, Yu Chih.

In: Drug and Chemical Toxicology, Vol. 36, No. 3, 07.2013, p. 313-319.

Research output: Contribution to journalArticle

Wu, Shing Sheng ; Chen, Lih Geeng ; Lin, Ren-Jye ; Lin, Shyr Yi ; Lo, Yueh E. ; Liang, Yu Chih. / Cytotoxicity of (-)-vitisin B in human leukemia cells. In: Drug and Chemical Toxicology. 2013 ; Vol. 36, No. 3. pp. 313-319.
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AU - Liang, Yu Chih

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