Cytotoxicity of indocyanine green on retinal pigment epithelium

Implications for macular hole surgery

Jau D. Ho, R. J F Tsai, San N. Chen, Hung Chiao Chen

Research output: Contribution to journalArticle

96 Citations (Scopus)

Abstract

Objective: To evaluate the potential cytotoxic effects of indocyanine green (ICG) on cultured human retinal pigment epithelium (RPE) and the resultant implications for macular hole surgery. Methods: Human RPE cells were exposed to ICG in concentrations from 0.001 to 5 mg/mL. The exposure duration ranged from 5 minutes to 3 hours. Light microscopy, MTS viability assay, and calcein AM-ethidium homodimer 1 staining were used to evaluate the cytotoxic effects of ICG. Results: The RPE cells incubated with up to 5 mg/mL of ICG for 5 minutes or less exhibited no morphologic change and no significant decrease in dehydrogenase activity. When RPE cells were exposed to 5 mg/mL of ICG for 10 minutes, 1 mg/mL of ICG for 20 minutes, or 0.01 mg/mL of ICG for 3 hours, cell morphologic features were altered, mitochondrial dehydrogenase activity decreased, and some cells were necrotic. Conclusions: Indocyanine green caused cytotoxicity in cultured human RPE in a dose- and time-dependent manner. Cell death occurred by necrosis. Clinical Relevance: Exposure of RPE cells to ICG concentrations up to 5 mg/mL for 5 minutes or less was not injurious; prolonged exposure to a low ICG concentration was toxic. Since ICG may be retained in the vitreous cavity for a lengthy period, thorough washout of ICG during macular hole surgery is required.

Original languageEnglish
Pages (from-to)1423-1429
Number of pages7
JournalArchives of Ophthalmology
Volume121
Issue number10
DOIs
Publication statusPublished - Oct 2003
Externally publishedYes

Fingerprint

Retinal Perforations
Indocyanine Green
Retinal Pigment Epithelium
Oxidoreductases
Poisons
Microscopy
Cell Death
Necrosis

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Cytotoxicity of indocyanine green on retinal pigment epithelium : Implications for macular hole surgery. / Ho, Jau D.; Tsai, R. J F; Chen, San N.; Chen, Hung Chiao.

In: Archives of Ophthalmology, Vol. 121, No. 10, 10.2003, p. 1423-1429.

Research output: Contribution to journalArticle

Ho, Jau D. ; Tsai, R. J F ; Chen, San N. ; Chen, Hung Chiao. / Cytotoxicity of indocyanine green on retinal pigment epithelium : Implications for macular hole surgery. In: Archives of Ophthalmology. 2003 ; Vol. 121, No. 10. pp. 1423-1429.
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