Abstract
The cell cytotoxicity of nanoparticles (NPs) and particle-bound polycyclic aromatic hydrocarbons (p-PAHs) in emission of a four-stroke motorcycle (4-st/mc) was studied. Cell cytotoxicity was evaluated by detecting the cell viability rate (3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide, MTT assay) of carcinomic human airway alveolar epithelial cells (A549) and the content of cytokine (IL-8) in the mucus of A549 after exposing to NPs and p-PAHs. In experiment, as the speed accelerated (15 km/h and 30 km/h), the concentration of high molecular weight PAHs (HMW-PAHs) increased. According to the result of MTT assay, the cell viability rate of A549 exposed to the NPs exhausted from idle condition was lower than that from 15 km/h and 30 km/h speed. However, the cell viability rate of A549 stimulated by p-PAHs exhausted from the 30 km/h condition was 15.9%, which was lower than 31.4% (idle) and 20.2% (15 km/h). It means the p-PAHs might be the main factor to induce the death of cell in the high speed condition. Moreover, p-PAHs exhausted from the idle condition could cause more serious inflammation in the A549 cells than that from other speeds.
| Original language | English |
|---|---|
| Title of host publication | Nanotechnology 2010 |
| Subtitle of host publication | Bio Sensors, Instruments, Medical, Environment and Energy - Technical Proceedings of the 2010 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2010 |
| Pages | 587-590 |
| Number of pages | 4 |
| Volume | 3 |
| Publication status | Published - 2010 |
| Externally published | Yes |
| Event | Nanotechnology 2010: Bio Sensors, Instruments, Medical, Environment and Energy - 2010 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2010 - Anaheim, CA, United States Duration: Jun 21 2010 → Jun 24 2010 |
Conference
| Conference | Nanotechnology 2010: Bio Sensors, Instruments, Medical, Environment and Energy - 2010 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2010 |
|---|---|
| Country | United States |
| City | Anaheim, CA |
| Period | 6/21/10 → 6/24/10 |
Fingerprint
Keywords
- Cytotoxicity
- IL-8
- Motorcycle
- MTT assay
- Particle-PAHs
ASJC Scopus subject areas
- Bioengineering
- Biomedical Engineering
- Electrical and Electronic Engineering
Cite this
Cytotoxicity assessment of nanoparticles and polycyclic aromatic hydrocarbons exhausted from motorcycle. / Chien, S. M.; Huang, Yuh Jeen; Chuang, C. Y.; Hsiao, I. L.
Nanotechnology 2010: Bio Sensors, Instruments, Medical, Environment and Energy - Technical Proceedings of the 2010 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2010. Vol. 3 2010. p. 587-590.Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
}
TY - GEN
T1 - Cytotoxicity assessment of nanoparticles and polycyclic aromatic hydrocarbons exhausted from motorcycle
AU - Chien, S. M.
AU - Huang, Yuh Jeen
AU - Chuang, C. Y.
AU - Hsiao, I. L.
PY - 2010
Y1 - 2010
N2 - The cell cytotoxicity of nanoparticles (NPs) and particle-bound polycyclic aromatic hydrocarbons (p-PAHs) in emission of a four-stroke motorcycle (4-st/mc) was studied. Cell cytotoxicity was evaluated by detecting the cell viability rate (3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide, MTT assay) of carcinomic human airway alveolar epithelial cells (A549) and the content of cytokine (IL-8) in the mucus of A549 after exposing to NPs and p-PAHs. In experiment, as the speed accelerated (15 km/h and 30 km/h), the concentration of high molecular weight PAHs (HMW-PAHs) increased. According to the result of MTT assay, the cell viability rate of A549 exposed to the NPs exhausted from idle condition was lower than that from 15 km/h and 30 km/h speed. However, the cell viability rate of A549 stimulated by p-PAHs exhausted from the 30 km/h condition was 15.9%, which was lower than 31.4% (idle) and 20.2% (15 km/h). It means the p-PAHs might be the main factor to induce the death of cell in the high speed condition. Moreover, p-PAHs exhausted from the idle condition could cause more serious inflammation in the A549 cells than that from other speeds.
AB - The cell cytotoxicity of nanoparticles (NPs) and particle-bound polycyclic aromatic hydrocarbons (p-PAHs) in emission of a four-stroke motorcycle (4-st/mc) was studied. Cell cytotoxicity was evaluated by detecting the cell viability rate (3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide, MTT assay) of carcinomic human airway alveolar epithelial cells (A549) and the content of cytokine (IL-8) in the mucus of A549 after exposing to NPs and p-PAHs. In experiment, as the speed accelerated (15 km/h and 30 km/h), the concentration of high molecular weight PAHs (HMW-PAHs) increased. According to the result of MTT assay, the cell viability rate of A549 exposed to the NPs exhausted from idle condition was lower than that from 15 km/h and 30 km/h speed. However, the cell viability rate of A549 stimulated by p-PAHs exhausted from the 30 km/h condition was 15.9%, which was lower than 31.4% (idle) and 20.2% (15 km/h). It means the p-PAHs might be the main factor to induce the death of cell in the high speed condition. Moreover, p-PAHs exhausted from the idle condition could cause more serious inflammation in the A549 cells than that from other speeds.
KW - Cytotoxicity
KW - IL-8
KW - Motorcycle
KW - MTT assay
KW - Particle-PAHs
UR - http://www.scopus.com/inward/record.url?scp=78049440136&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=78049440136&partnerID=8YFLogxK
M3 - Conference contribution
SN - 9781439834152
VL - 3
SP - 587
EP - 590
BT - Nanotechnology 2010
ER -