Cytotoxicity assessment of nanoparticles and polycyclic aromatic hydrocarbons exhausted from motorcycle

S. M. Chien; Yuh Jeen Huang; C. Y. Chuang; I. L. Hsiao

Cytotoxicity assessment of nanoparticles and polycyclic aromatic hydrocarbons exhausted from motorcycle

S. M. Chien, Yuh Jeen Huang, C. Y. Chuang, I. L. Hsiao

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

The cell cytotoxicity of nanoparticles (NPs) and particle-bound polycyclic aromatic hydrocarbons (p-PAHs) in emission of a four-stroke motorcycle (4-st/mc) was studied. Cell cytotoxicity was evaluated by detecting the cell viability rate (3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide, MTT assay) of carcinomic human airway alveolar epithelial cells (A549) and the content of cytokine (IL-8) in the mucus of A549 after exposing to NPs and p-PAHs. In experiment, as the speed accelerated (15 km/h and 30 km/h), the concentration of high molecular weight PAHs (HMW-PAHs) increased. According to the result of MTT assay, the cell viability rate of A549 exposed to the NPs exhausted from idle condition was lower than that from 15 km/h and 30 km/h speed. However, the cell viability rate of A549 stimulated by p-PAHs exhausted from the 30 km/h condition was 15.9%, which was lower than 31.4% (idle) and 20.2% (15 km/h). It means the p-PAHs might be the main factor to induce the death of cell in the high speed condition. Moreover, p-PAHs exhausted from the idle condition could cause more serious inflammation in the A549 cells than that from other speeds.

Original language	English
Title of host publication	Nanotechnology 2010
Subtitle of host publication	Bio Sensors, Instruments, Medical, Environment and Energy - Technical Proceedings of the 2010 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2010
Pages	587-590
Number of pages	4
Volume	3
Publication status	Published - 2010
Externally published	Yes
Event	Nanotechnology 2010: Bio Sensors, Instruments, Medical, Environment and Energy - 2010 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2010 - Anaheim, CA, United States Duration: Jun 21 2010 → Jun 24 2010

Conference

Conference	Nanotechnology 2010: Bio Sensors, Instruments, Medical, Environment and Energy - 2010 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2010
Country	United States
City	Anaheim, CA
Period	6/21/10 → 6/24/10

Keywords

Cytotoxicity
IL-8
Motorcycle
MTT assay
Particle-PAHs

ASJC Scopus subject areas

Bioengineering
Biomedical Engineering
Electrical and Electronic Engineering

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Cite this

Chien, S. M., Huang, Y. J., Chuang, C. Y., & Hsiao, I. L. (2010). Cytotoxicity assessment of nanoparticles and polycyclic aromatic hydrocarbons exhausted from motorcycle. In Nanotechnology 2010: Bio Sensors, Instruments, Medical, Environment and Energy - Technical Proceedings of the 2010 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2010 (Vol. 3, pp. 587-590)

Cytotoxicity assessment of nanoparticles and polycyclic aromatic hydrocarbons exhausted from motorcycle. / Chien, S. M.; Huang, Yuh Jeen; Chuang, C. Y.; Hsiao, I. L.

Nanotechnology 2010: Bio Sensors, Instruments, Medical, Environment and Energy - Technical Proceedings of the 2010 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2010. Vol. 3 2010. p. 587-590.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Chien, SM, Huang, YJ, Chuang, CY & Hsiao, IL 2010, Cytotoxicity assessment of nanoparticles and polycyclic aromatic hydrocarbons exhausted from motorcycle. in Nanotechnology 2010: Bio Sensors, Instruments, Medical, Environment and Energy - Technical Proceedings of the 2010 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2010. vol. 3, pp. 587-590, Nanotechnology 2010: Bio Sensors, Instruments, Medical, Environment and Energy - 2010 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2010, Anaheim, CA, United States, 6/21/10.

Chien, S. M. ; Huang, Yuh Jeen ; Chuang, C. Y. ; Hsiao, I. L. / Cytotoxicity assessment of nanoparticles and polycyclic aromatic hydrocarbons exhausted from motorcycle. Nanotechnology 2010: Bio Sensors, Instruments, Medical, Environment and Energy - Technical Proceedings of the 2010 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2010. Vol. 3 2010. pp. 587-590

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abstract = "The cell cytotoxicity of nanoparticles (NPs) and particle-bound polycyclic aromatic hydrocarbons (p-PAHs) in emission of a four-stroke motorcycle (4-st/mc) was studied. Cell cytotoxicity was evaluated by detecting the cell viability rate (3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide, MTT assay) of carcinomic human airway alveolar epithelial cells (A549) and the content of cytokine (IL-8) in the mucus of A549 after exposing to NPs and p-PAHs. In experiment, as the speed accelerated (15 km/h and 30 km/h), the concentration of high molecular weight PAHs (HMW-PAHs) increased. According to the result of MTT assay, the cell viability rate of A549 exposed to the NPs exhausted from idle condition was lower than that from 15 km/h and 30 km/h speed. However, the cell viability rate of A549 stimulated by p-PAHs exhausted from the 30 km/h condition was 15.9%, which was lower than 31.4% (idle) and 20.2% (15 km/h). It means the p-PAHs might be the main factor to induce the death of cell in the high speed condition. Moreover, p-PAHs exhausted from the idle condition could cause more serious inflammation in the A549 cells than that from other speeds.",

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AB - The cell cytotoxicity of nanoparticles (NPs) and particle-bound polycyclic aromatic hydrocarbons (p-PAHs) in emission of a four-stroke motorcycle (4-st/mc) was studied. Cell cytotoxicity was evaluated by detecting the cell viability rate (3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide, MTT assay) of carcinomic human airway alveolar epithelial cells (A549) and the content of cytokine (IL-8) in the mucus of A549 after exposing to NPs and p-PAHs. In experiment, as the speed accelerated (15 km/h and 30 km/h), the concentration of high molecular weight PAHs (HMW-PAHs) increased. According to the result of MTT assay, the cell viability rate of A549 exposed to the NPs exhausted from idle condition was lower than that from 15 km/h and 30 km/h speed. However, the cell viability rate of A549 stimulated by p-PAHs exhausted from the 30 km/h condition was 15.9%, which was lower than 31.4% (idle) and 20.2% (15 km/h). It means the p-PAHs might be the main factor to induce the death of cell in the high speed condition. Moreover, p-PAHs exhausted from the idle condition could cause more serious inflammation in the A549 cells than that from other speeds.

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