Cytotoxic activity of sesquiterpenoids from Atractylodes ovata on leukemia cell lines

Ching Chiung Wang, Lih Geeng Chen, Ling-Ling Yang

Research output: Contribution to journalArticle

59 Citations (Scopus)

Abstract

The rhizome of Atractylodes ovata (Bai Zhu in Chinese) is a widely used traditional Chinese herb in Taiwan as a tonic agent. In this paper, four sesquiterpenoids, namely atractylon, and atractylenolides I, II, and III, were isolated from the n-hexane extract of A. ovata and were evaluated for cytotoxic effects in vitro. Atractylon significantly inhibited the growth of human leukemia cell line HL-60 and mouse leukemia cell line P-388, and showed low cytotoxicity against primary cultures of normal human peripheral blood mononuclear cells at 15 μg/ml for 12 h. Atractylon had a dose-dependent antiproliferative effect on the two tumor cell lines. In accordance with DNA fragment increases and PARP protein decreases, atractylon at 15 μg/ml for 6 h induced apoptosis in HL-60 cells. Moreover, atractylon inhibited the viability of P-388 cells and induced apoptosis after 15 μg/ml treatment for 12 h in an in vitro assay. However, atractylenolide 1 at 30 μg/ml for 12 h also induced apoptosis in HL-60 and P-388 cells, but atractylenolides II and III showed no significant inhibition effects on tumor cell growth. As the above results suggested, atractylon and atractylenolide I were the major cytotoxic principle constituents of A. ovata on leukemia cell lines.

Original languageEnglish
Pages (from-to)204-208
Number of pages5
JournalPlanta Medica
Volume68
Issue number3
DOIs
Publication statusPublished - 2002

Keywords

  • Apoptosis
  • Atractylenolide I
  • Atractylodes ovata (Bai Zhu)
  • Atractylon
  • Compositae
  • Cytotoxicity
  • HL-60
  • P-388

ASJC Scopus subject areas

  • Plant Science
  • Drug Discovery
  • Organic Chemistry
  • Pharmacology

Fingerprint Dive into the research topics of 'Cytotoxic activity of sesquiterpenoids from Atractylodes ovata on leukemia cell lines'. Together they form a unique fingerprint.

  • Cite this