Cytoprotective Response of A1, a Bcl-2 Homologue Expressed in Mature Human Neutrophils and Promyelocytic HL-60 Cells, to Oxidant Stress-Induced Cell Death

Chien Ying Liu, Peter I. Chuang, Chun Liang Chou, Shu Min Lin, Hao Cheng Chen, Paichien Chou, Yun Hen Liu, Chih Ten Yu, Chun Hua Wang, Horng Chyuan Lin, Han Pin Kuo

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The ability to generate reactive oxidative intermediates is one of the quintessential properties of mature human neutrophils. Endogenously generated oxidants have been shown to be an important mechanism underlying neutrophil cell death. In acute lung inflammation, newly recruited neutrophils further encounter external oxidants, including reactive oxygen and nitrogen intermediates. In our present study, we showed that A1, a constitutive and inducible Bcl-2 homologue expressed in mature circulating human neutrophils, might confer the protection from hydrogen peroxide (H2O2)- and peroxynitrite (ONOO)-induced cell death. Utilizing the myeloid precursor cell line, HL-60, we further examined the hypothesis that A1 was capable of conferring cytoprotective activity against these oxidative stresses. Whereas the control-transfected HL-60 cells expressed small amounts of A1 and were sensitive to the biologically relevant, cell death-inducing oxidants, H 2O2 and ONOO, the stable transfectants that overexpressed A1 were significantly more tolerant. Furthermore, there was a correlation between the level of A1 expression and the antiapoptotic activity. Thus, our results suggest a cytoprotective role of A1 in mature human neutrophils under oxidant stresses in host defense and inflammation.

Original languageEnglish
Pages (from-to)214-227
Number of pages14
JournalJournal of Biomedical Science
Issue number2
Publication statusPublished - Mar 11 2004
Externally publishedYes



  • A1
  • Apoptosis
  • Cell death
  • HL-60 cells
  • Neutrophils
  • Reactive oxygen intermediate

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Molecular Biology
  • Clinical Biochemistry
  • Cell Biology
  • Biochemistry, medical
  • Pharmacology (medical)

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