Cytoprotective effects of phenolic acids on methylglyoxal-induced apoptosis in Neuro-2A cells

Shang Ming Huang, Hong Chih Chuang, Chi Hao Wu, Gow Chin Yen

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

In the process of glycation, methylglyoxal is a reactive dicarbonyl compound physiologically generated as an intermediate of glycolysis, and is found in high levels in blood or tissue of diabetic models. Biological glycation has been commonly implicated in the development of diabetic microvascular complications of neuropathy. Increasing evidence suggests that neuronal cell cycle regulatory failure followed by apoptosis is an important mechanism in the development of diabetic neuropathy complication. Naturally occurring antioxidants, especially phenolic acids have been recommended as the major bioactive compounds to prevent chronic diseases and promote health benefits. The objective of this study was to investigate the inhibitory abilities of phenolic acids (chlorogenic acid, syringic acid and vanillic acid) on methylglyoxal-induced mouse Neuro-2A neuroblastoma (Neuro-2A) cell apoptosis in the progression of diabetic neuropathy. The data indicated that methylglyoxal induced mouse Neuro-2A neuroblastoma (Neuro-2A) cell apoptosis via alternation of mitochondria membrane potential and Bax/Bcl-2 ratio, activation of caspase-3, and cleavage of poly (ADP-ribose) polymerase. Furthermore, the results demonstrated that activation of mitogen-activated protein kinase signal pathways (JNK and p38) participated in the methylglyoxal-induced Neuro-2A cell apoptosis process. Treatment of Neuro-2A cells with phenolic acids markedly suppresses cell apoptosis induced by methylglyoxal, suggesting that phenolic acids possess cytoprotective ability in the prevention of diabetic neuropathy complication.

Original languageEnglish
Pages (from-to)940-949
Number of pages10
JournalMolecular Nutrition and Food Research
Volume52
Issue number8
DOIs
Publication statusPublished - Aug 2008
Externally publishedYes

Fingerprint

Pyruvaldehyde
phenolic acids
diabetic neuropathy
apoptosis
Diabetic Neuropathies
Apoptosis
Diabetes Complications
glycation
Neuroblastoma
mitogen-activated protein kinase
cells
Vanillic Acid
syringic acid
Chlorogenic Acid
NAD ADP-ribosyltransferase
vanillic acid
peripheral nervous system diseases
Poly(ADP-ribose) Polymerases
mice
glycolysis

Keywords

  • Cytoprotection
  • Glycation
  • Methylglyoxal
  • Neuro-2A cells
  • Phenolic acids

ASJC Scopus subject areas

  • Food Science
  • Biotechnology
  • Medicine(all)

Cite this

Cytoprotective effects of phenolic acids on methylglyoxal-induced apoptosis in Neuro-2A cells. / Huang, Shang Ming; Chuang, Hong Chih; Wu, Chi Hao; Yen, Gow Chin.

In: Molecular Nutrition and Food Research, Vol. 52, No. 8, 08.2008, p. 940-949.

Research output: Contribution to journalArticle

Huang, Shang Ming ; Chuang, Hong Chih ; Wu, Chi Hao ; Yen, Gow Chin. / Cytoprotective effects of phenolic acids on methylglyoxal-induced apoptosis in Neuro-2A cells. In: Molecular Nutrition and Food Research. 2008 ; Vol. 52, No. 8. pp. 940-949.
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