Cytokine-induced killer T cells enhance the cytotoxicity against carboplatin-resistant ovarian cancer cells

Yueh Pan, Ya Hsu Chiu, Shao Chih Chiu, Der Yang Cho, Liang Ming Lee, Yu Ching Wen, Jacqueline Whang-Peng, Chi Hao Hsiao, Ping Hsiao Shih

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Background/Aim: Ovarian cancer (OC) is typically diagnosed at an advanced stage with limitations for cure. Cytokine-induced killer (CIK) T cell therapy exerts significant cytotoxic effects against cancer cells and reduces the adverse effects of chemotherapy. Herein, we performed a flow cytometry-based method to evaluate the cytotoxicity of peripheral blood mononuclear cells-derived CIK cells against OC cells. Materials and Methods: The CIK cells were induced and expanded using an interferon-γ/IL-2-based xeno-free medium system. The cytotoxicity of CIK cells or carboplatin against OC cells was examined. Results: The CIK cells showed an NK-like phenotypic characteristic and dose-dependently increased cytotoxicity against OC cells. We found that the number of advanced OC cells, which were more resistant to carboplatin, was dramatically decreased by an additional one-shot CIK treatment. Conclusion: CIK cells have a potent cytotoxic ability that would be explored as an alternative strategy for cancer treatment in the near future.

Original languageEnglish
Pages (from-to)3865-3872
Number of pages8
JournalAnticancer Research
Volume40
Issue number7
DOIs
Publication statusPublished - Jul 2020

Keywords

  • Chemotherapy
  • Cytokine-induced killer cell
  • Flow cytometry
  • Ovarian cancer
  • Peripheral blood mononuclear cell

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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