Cystometric measurements in rats with an experimentally induced traumatic brain injury and voiding dysfunction: A time-course study

Chellappan Praveen Rajneesh, Ling Yu Yang, Shih Ching Chen, Tsung Hsun Hsieh, Hung Yen Chin, Chih Wei Peng

Research output: Contribution to journalArticle

Abstract

Traumatic brain injuries (TBIs) are a serious public health issue worldwide with increased mortality as well as severe disabilities and injuries caused by falls and road accidents. Unfortunately, there is no approved therapy for TBIs, and bladder dysfunction is a striking symptom. Accordingly, we attempted to analyze bladder dysfunction and voiding efficiency in rats with a TBI at different time-course intervals. Time-dependent analyses were scheduled from the next day until four weeks after a TBI. Experimental animals were grouped and analyzed under the above conditions. Cystometric measurements were used for this analysis and were further elaborated as external urethral sphincter electromyographic (EUS-EMG) activity and cystometrogram (CMG) measurements. Moreover, magnetic resonance imaging (MRI) studies were conducted to investigate secondary injury progression in TBI rats, and results were compared to normal control (NC) rats. Results of EUS-EMG revealed that the burst period, active period, and silent period in TBI rats were drastically reduced compared to NC rats, but they increased later and reached a stagnant phase. Likewise, in CMG measurements, bladder function, the voided volume, and voiding efficiency decreased immediately after the TBI, and other parameters like the volume threshold, inter-contraction interval, and residual volume drastically increased. Later, those levels changed, and all observed results were compared to NC rats. MRI results revealed the prevalence of cerebral edema and the progression of secondary injury. All of the above-stated results of the experiments were extensively substantiated. Thus, these innovative findings of our study model will surely pave the way for new therapeutic interventions for TBI treatment and prominently highlight their applications in the field of neuroscience in the future.

Original languageEnglish
Article number325
JournalBrain Sciences
Volume9
Issue number11
DOIs
Publication statusPublished - Nov 2019

Fingerprint

Urinary Bladder
Urethra
Wounds and Injuries
Magnetic Resonance Imaging
Residual Volume
Traumatic Brain Injury
Brain Edema
Neurosciences
Accidents
Public Health
Mortality
Therapeutics

Keywords

  • Cystometric measurements
  • External urethral sphincter electromyographic activity
  • Time-course analysis
  • Traumatic brain injury

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Cystometric measurements in rats with an experimentally induced traumatic brain injury and voiding dysfunction : A time-course study. / Rajneesh, Chellappan Praveen; Yang, Ling Yu; Chen, Shih Ching; Hsieh, Tsung Hsun; Chin, Hung Yen; Peng, Chih Wei.

In: Brain Sciences, Vol. 9, No. 11, 325, 11.2019.

Research output: Contribution to journalArticle

@article{a04019c6e576462da34095d2127d2b39,
title = "Cystometric measurements in rats with an experimentally induced traumatic brain injury and voiding dysfunction: A time-course study",
abstract = "Traumatic brain injuries (TBIs) are a serious public health issue worldwide with increased mortality as well as severe disabilities and injuries caused by falls and road accidents. Unfortunately, there is no approved therapy for TBIs, and bladder dysfunction is a striking symptom. Accordingly, we attempted to analyze bladder dysfunction and voiding efficiency in rats with a TBI at different time-course intervals. Time-dependent analyses were scheduled from the next day until four weeks after a TBI. Experimental animals were grouped and analyzed under the above conditions. Cystometric measurements were used for this analysis and were further elaborated as external urethral sphincter electromyographic (EUS-EMG) activity and cystometrogram (CMG) measurements. Moreover, magnetic resonance imaging (MRI) studies were conducted to investigate secondary injury progression in TBI rats, and results were compared to normal control (NC) rats. Results of EUS-EMG revealed that the burst period, active period, and silent period in TBI rats were drastically reduced compared to NC rats, but they increased later and reached a stagnant phase. Likewise, in CMG measurements, bladder function, the voided volume, and voiding efficiency decreased immediately after the TBI, and other parameters like the volume threshold, inter-contraction interval, and residual volume drastically increased. Later, those levels changed, and all observed results were compared to NC rats. MRI results revealed the prevalence of cerebral edema and the progression of secondary injury. All of the above-stated results of the experiments were extensively substantiated. Thus, these innovative findings of our study model will surely pave the way for new therapeutic interventions for TBI treatment and prominently highlight their applications in the field of neuroscience in the future.",
keywords = "Cystometric measurements, External urethral sphincter electromyographic activity, Time-course analysis, Traumatic brain injury",
author = "Rajneesh, {Chellappan Praveen} and Yang, {Ling Yu} and Chen, {Shih Ching} and Hsieh, {Tsung Hsun} and Chin, {Hung Yen} and Peng, {Chih Wei}",
year = "2019",
month = "11",
doi = "10.3390/brainsci9110325",
language = "English",
volume = "9",
journal = "Brain Sciences",
issn = "2076-3425",
publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",
number = "11",

}

TY - JOUR

T1 - Cystometric measurements in rats with an experimentally induced traumatic brain injury and voiding dysfunction

T2 - A time-course study

AU - Rajneesh, Chellappan Praveen

AU - Yang, Ling Yu

AU - Chen, Shih Ching

AU - Hsieh, Tsung Hsun

AU - Chin, Hung Yen

AU - Peng, Chih Wei

PY - 2019/11

Y1 - 2019/11

N2 - Traumatic brain injuries (TBIs) are a serious public health issue worldwide with increased mortality as well as severe disabilities and injuries caused by falls and road accidents. Unfortunately, there is no approved therapy for TBIs, and bladder dysfunction is a striking symptom. Accordingly, we attempted to analyze bladder dysfunction and voiding efficiency in rats with a TBI at different time-course intervals. Time-dependent analyses were scheduled from the next day until four weeks after a TBI. Experimental animals were grouped and analyzed under the above conditions. Cystometric measurements were used for this analysis and were further elaborated as external urethral sphincter electromyographic (EUS-EMG) activity and cystometrogram (CMG) measurements. Moreover, magnetic resonance imaging (MRI) studies were conducted to investigate secondary injury progression in TBI rats, and results were compared to normal control (NC) rats. Results of EUS-EMG revealed that the burst period, active period, and silent period in TBI rats were drastically reduced compared to NC rats, but they increased later and reached a stagnant phase. Likewise, in CMG measurements, bladder function, the voided volume, and voiding efficiency decreased immediately after the TBI, and other parameters like the volume threshold, inter-contraction interval, and residual volume drastically increased. Later, those levels changed, and all observed results were compared to NC rats. MRI results revealed the prevalence of cerebral edema and the progression of secondary injury. All of the above-stated results of the experiments were extensively substantiated. Thus, these innovative findings of our study model will surely pave the way for new therapeutic interventions for TBI treatment and prominently highlight their applications in the field of neuroscience in the future.

AB - Traumatic brain injuries (TBIs) are a serious public health issue worldwide with increased mortality as well as severe disabilities and injuries caused by falls and road accidents. Unfortunately, there is no approved therapy for TBIs, and bladder dysfunction is a striking symptom. Accordingly, we attempted to analyze bladder dysfunction and voiding efficiency in rats with a TBI at different time-course intervals. Time-dependent analyses were scheduled from the next day until four weeks after a TBI. Experimental animals were grouped and analyzed under the above conditions. Cystometric measurements were used for this analysis and were further elaborated as external urethral sphincter electromyographic (EUS-EMG) activity and cystometrogram (CMG) measurements. Moreover, magnetic resonance imaging (MRI) studies were conducted to investigate secondary injury progression in TBI rats, and results were compared to normal control (NC) rats. Results of EUS-EMG revealed that the burst period, active period, and silent period in TBI rats were drastically reduced compared to NC rats, but they increased later and reached a stagnant phase. Likewise, in CMG measurements, bladder function, the voided volume, and voiding efficiency decreased immediately after the TBI, and other parameters like the volume threshold, inter-contraction interval, and residual volume drastically increased. Later, those levels changed, and all observed results were compared to NC rats. MRI results revealed the prevalence of cerebral edema and the progression of secondary injury. All of the above-stated results of the experiments were extensively substantiated. Thus, these innovative findings of our study model will surely pave the way for new therapeutic interventions for TBI treatment and prominently highlight their applications in the field of neuroscience in the future.

KW - Cystometric measurements

KW - External urethral sphincter electromyographic activity

KW - Time-course analysis

KW - Traumatic brain injury

UR - http://www.scopus.com/inward/record.url?scp=85075207488&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85075207488&partnerID=8YFLogxK

U2 - 10.3390/brainsci9110325

DO - 10.3390/brainsci9110325

M3 - Article

AN - SCOPUS:85075207488

VL - 9

JO - Brain Sciences

JF - Brain Sciences

SN - 2076-3425

IS - 11

M1 - 325

ER -