CYP1A1 protein activity is associated with allelic variation in pterygium tissues and cells

Mei Ling Peng, Yi Yu Tsai, Chun Chi Chiang, Ying Che Huang, Ming Chih Chou, Kun Tu Yeh, Huei Lee, Ya Wen Cheng

Research output: Contribution to journalArticle

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Abstract

Background: A thymine/cytosine point mutation in the MSP I restriction site of cytochrome P450 1A1 (CYP1A1) has been linked to susceptibility to smoking-related cancers and is reported to result in increased enzyme activity. Therefore, we sought to determine whether allelic variation of CYP1A1 is associated with protein expression and protein activity in pterygium. Methods: We collected 150 pterygium samples and 50 normal conjunctiva samples, which served as controls. DNA samples were extracted from blood cells and then subjected to real-time ploymerase chain reaction (PCR) to determine CYP1A1 genotype. CYP1A1 protein expression was determined by immunohistochemical staining with a monoclonal antibody for CYP1A1. Pterygium epithelial cells (PECs), cultured in a serum-free culture medium, real-time PCR, western blot and enzyme-linked immunosorbent assay (ELISA) were used to understand the effect of CYP1A1 allelic variation in protein expression and activity. Results: Forty-eight (33.3%) pterygium specimens tested positive for CYP1A1 protein expression. CYP1A1 protein expression was significantly greater in the pterygium group than in the control group (p<0.0001). In addition, CYP1A1 protein expression was associated with allelic variation. CYP1A1 protein expression was significantly greater in the m2/ m2 group than in the m1/m1and m1/m2 groups (p=0.006). In the cell model, CYP1A1 protein expression and b[a]P 7,8- diol 9,10-epoxide (BPDE)-like DNA adducts increased in CYP1A1 m2/m2 (genotype T/T) PEC cells as compared with m1/m2 (genotype C/T) and m1/m1 (genotype C/C) cells. Conclusions: CYP1A1 expression in pterygium correlates with allelic variation and can be used as an independent risk marker. © 2012 Molecular Vision.

Original languageEnglish
Pages (from-to)1937-1943
Number of pages7
JournalMolecular Vision
Volume18
Publication statusPublished - 2012

Fingerprint

Pterygium
Cytochrome P-450 Enzyme System
Proteins
Genotype
Epithelial Cells
Thymine
DNA Adducts
Conjunctiva
Cytosine
Epoxy Compounds
Serum-Free Culture Media
Point Mutation

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Peng, M. L., Tsai, Y. Y., Chiang, C. C., Huang, Y. C., Chou, M. C., Yeh, K. T., ... Cheng, Y. W. (2012). CYP1A1 protein activity is associated with allelic variation in pterygium tissues and cells. Molecular Vision, 18, 1937-1943.

CYP1A1 protein activity is associated with allelic variation in pterygium tissues and cells. / Peng, Mei Ling; Tsai, Yi Yu; Chiang, Chun Chi; Huang, Ying Che; Chou, Ming Chih; Yeh, Kun Tu; Lee, Huei; Cheng, Ya Wen.

In: Molecular Vision, Vol. 18, 2012, p. 1937-1943.

Research output: Contribution to journalArticle

Peng, ML, Tsai, YY, Chiang, CC, Huang, YC, Chou, MC, Yeh, KT, Lee, H & Cheng, YW 2012, 'CYP1A1 protein activity is associated with allelic variation in pterygium tissues and cells', Molecular Vision, vol. 18, pp. 1937-1943.
Peng ML, Tsai YY, Chiang CC, Huang YC, Chou MC, Yeh KT et al. CYP1A1 protein activity is associated with allelic variation in pterygium tissues and cells. Molecular Vision. 2012;18:1937-1943.
Peng, Mei Ling ; Tsai, Yi Yu ; Chiang, Chun Chi ; Huang, Ying Che ; Chou, Ming Chih ; Yeh, Kun Tu ; Lee, Huei ; Cheng, Ya Wen. / CYP1A1 protein activity is associated with allelic variation in pterygium tissues and cells. In: Molecular Vision. 2012 ; Vol. 18. pp. 1937-1943.
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abstract = "Background: A thymine/cytosine point mutation in the MSP I restriction site of cytochrome P450 1A1 (CYP1A1) has been linked to susceptibility to smoking-related cancers and is reported to result in increased enzyme activity. Therefore, we sought to determine whether allelic variation of CYP1A1 is associated with protein expression and protein activity in pterygium. Methods: We collected 150 pterygium samples and 50 normal conjunctiva samples, which served as controls. DNA samples were extracted from blood cells and then subjected to real-time ploymerase chain reaction (PCR) to determine CYP1A1 genotype. CYP1A1 protein expression was determined by immunohistochemical staining with a monoclonal antibody for CYP1A1. Pterygium epithelial cells (PECs), cultured in a serum-free culture medium, real-time PCR, western blot and enzyme-linked immunosorbent assay (ELISA) were used to understand the effect of CYP1A1 allelic variation in protein expression and activity. Results: Forty-eight (33.3{\%}) pterygium specimens tested positive for CYP1A1 protein expression. CYP1A1 protein expression was significantly greater in the pterygium group than in the control group (p<0.0001). In addition, CYP1A1 protein expression was associated with allelic variation. CYP1A1 protein expression was significantly greater in the m2/ m2 group than in the m1/m1and m1/m2 groups (p=0.006). In the cell model, CYP1A1 protein expression and b[a]P 7,8- diol 9,10-epoxide (BPDE)-like DNA adducts increased in CYP1A1 m2/m2 (genotype T/T) PEC cells as compared with m1/m2 (genotype C/T) and m1/m1 (genotype C/C) cells. Conclusions: CYP1A1 expression in pterygium correlates with allelic variation and can be used as an independent risk marker. {\circledC} 2012 Molecular Vision.",
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AU - Peng, Mei Ling

AU - Tsai, Yi Yu

AU - Chiang, Chun Chi

AU - Huang, Ying Che

AU - Chou, Ming Chih

AU - Yeh, Kun Tu

AU - Lee, Huei

AU - Cheng, Ya Wen

PY - 2012

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N2 - Background: A thymine/cytosine point mutation in the MSP I restriction site of cytochrome P450 1A1 (CYP1A1) has been linked to susceptibility to smoking-related cancers and is reported to result in increased enzyme activity. Therefore, we sought to determine whether allelic variation of CYP1A1 is associated with protein expression and protein activity in pterygium. Methods: We collected 150 pterygium samples and 50 normal conjunctiva samples, which served as controls. DNA samples were extracted from blood cells and then subjected to real-time ploymerase chain reaction (PCR) to determine CYP1A1 genotype. CYP1A1 protein expression was determined by immunohistochemical staining with a monoclonal antibody for CYP1A1. Pterygium epithelial cells (PECs), cultured in a serum-free culture medium, real-time PCR, western blot and enzyme-linked immunosorbent assay (ELISA) were used to understand the effect of CYP1A1 allelic variation in protein expression and activity. Results: Forty-eight (33.3%) pterygium specimens tested positive for CYP1A1 protein expression. CYP1A1 protein expression was significantly greater in the pterygium group than in the control group (p<0.0001). In addition, CYP1A1 protein expression was associated with allelic variation. CYP1A1 protein expression was significantly greater in the m2/ m2 group than in the m1/m1and m1/m2 groups (p=0.006). In the cell model, CYP1A1 protein expression and b[a]P 7,8- diol 9,10-epoxide (BPDE)-like DNA adducts increased in CYP1A1 m2/m2 (genotype T/T) PEC cells as compared with m1/m2 (genotype C/T) and m1/m1 (genotype C/C) cells. Conclusions: CYP1A1 expression in pterygium correlates with allelic variation and can be used as an independent risk marker. © 2012 Molecular Vision.

AB - Background: A thymine/cytosine point mutation in the MSP I restriction site of cytochrome P450 1A1 (CYP1A1) has been linked to susceptibility to smoking-related cancers and is reported to result in increased enzyme activity. Therefore, we sought to determine whether allelic variation of CYP1A1 is associated with protein expression and protein activity in pterygium. Methods: We collected 150 pterygium samples and 50 normal conjunctiva samples, which served as controls. DNA samples were extracted from blood cells and then subjected to real-time ploymerase chain reaction (PCR) to determine CYP1A1 genotype. CYP1A1 protein expression was determined by immunohistochemical staining with a monoclonal antibody for CYP1A1. Pterygium epithelial cells (PECs), cultured in a serum-free culture medium, real-time PCR, western blot and enzyme-linked immunosorbent assay (ELISA) were used to understand the effect of CYP1A1 allelic variation in protein expression and activity. Results: Forty-eight (33.3%) pterygium specimens tested positive for CYP1A1 protein expression. CYP1A1 protein expression was significantly greater in the pterygium group than in the control group (p<0.0001). In addition, CYP1A1 protein expression was associated with allelic variation. CYP1A1 protein expression was significantly greater in the m2/ m2 group than in the m1/m1and m1/m2 groups (p=0.006). In the cell model, CYP1A1 protein expression and b[a]P 7,8- diol 9,10-epoxide (BPDE)-like DNA adducts increased in CYP1A1 m2/m2 (genotype T/T) PEC cells as compared with m1/m2 (genotype C/T) and m1/m1 (genotype C/C) cells. Conclusions: CYP1A1 expression in pterygium correlates with allelic variation and can be used as an independent risk marker. © 2012 Molecular Vision.

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