Cyclin D1 overexpression correlates with poor tumor differentiation and prognosis in gastric cancer

Yan Shen Shan, Hui Ping Hsu, Ming Derg Lai, Yu Hsuan Hung, Chih Yang Wang, Meng Chi Yen, Yi Ling Chen

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Overexpression of cyclin D is associated with the molecular tumorigenesis of gastric cancer. The purpose of the present study was to investigate the expression of cyclin D in human gastric cancer and to determine the potential correlations between cyclin D expression and clinicopathological characteristics of specific histological types, as well as its prognostic significance. In the present study, the expression of the cyclin D1 (CCND1), cyclin D2 (CCND2) and cyclin D3 (CCND3) genes in gastric cancer patients was explored using the Oncomine database, and their correlation with overall survival (OS) and progression-free survival (PFS) was evaluated using Kaplan-Meier analysis. The prognostic significance of CCND1 protein expression was evaluated by western blot analysis of 32 matched specimens of gastric adenocarcinomas and normal tissues obtained from patients treated at the National Cheng Kung University Hospital (Tainan, Taiwan). Analysis of the Oncomine cancer microarray database revealed that CCND1 gene expression was significantly increased in gastric intestinal-type adenocarcinoma, while CCND2 was significantly increased in diffuse gastric adenocarcinoma, gastric intestinal-type adenocarcinoma and gastric mixed adenocarcinoma. Kaplan-Meier analysis indicated that overexpression of CCND1 was associated with reduced OS and PFS. In addition, overexpression of CCND1 and downregulation of CCND2 were significantly correlated with receptor tyrosine-protein kinase erb-2-negative tumors and poor differentiation. The ratio of relative CCND1 expression (expressed as the CCND1/β-actin ratio) in tumor tissues compared with that in normal tissues was correlated with poor differentiation (P=0.0018). In summary, CCND1 overexpression is associated with shorter survival in patients with gastric cancer and with poorly differentiated tumors.

Original languageEnglish
Pages (from-to)4517-4526
Number of pages10
JournalOncology Letters
Volume14
Issue number4
DOIs
Publication statusPublished - Jan 1 2017
Externally publishedYes

Fingerprint

Cyclin D1
Disease-Free Survival
Stomach Neoplasms
Cyclin D2
Stomach
Cyclin D
Adenocarcinoma
Neoplasms
Kaplan-Meier Estimate
Focal Adhesion Kinase 1
Survival
bcl-1 Genes
Cyclin D3
Databases
Receptor Protein-Tyrosine Kinases
Taiwan
Publications
Actins
Carcinogenesis
Down-Regulation

Keywords

  • Cyclin D1
  • Gastric cancer
  • Histological type
  • Overall survival
  • Progression-free survival

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Cyclin D1 overexpression correlates with poor tumor differentiation and prognosis in gastric cancer. / Shan, Yan Shen; Hsu, Hui Ping; Lai, Ming Derg; Hung, Yu Hsuan; Wang, Chih Yang; Yen, Meng Chi; Chen, Yi Ling.

In: Oncology Letters, Vol. 14, No. 4, 01.01.2017, p. 4517-4526.

Research output: Contribution to journalArticle

Shan, Yan Shen ; Hsu, Hui Ping ; Lai, Ming Derg ; Hung, Yu Hsuan ; Wang, Chih Yang ; Yen, Meng Chi ; Chen, Yi Ling. / Cyclin D1 overexpression correlates with poor tumor differentiation and prognosis in gastric cancer. In: Oncology Letters. 2017 ; Vol. 14, No. 4. pp. 4517-4526.
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abstract = "Overexpression of cyclin D is associated with the molecular tumorigenesis of gastric cancer. The purpose of the present study was to investigate the expression of cyclin D in human gastric cancer and to determine the potential correlations between cyclin D expression and clinicopathological characteristics of specific histological types, as well as its prognostic significance. In the present study, the expression of the cyclin D1 (CCND1), cyclin D2 (CCND2) and cyclin D3 (CCND3) genes in gastric cancer patients was explored using the Oncomine database, and their correlation with overall survival (OS) and progression-free survival (PFS) was evaluated using Kaplan-Meier analysis. The prognostic significance of CCND1 protein expression was evaluated by western blot analysis of 32 matched specimens of gastric adenocarcinomas and normal tissues obtained from patients treated at the National Cheng Kung University Hospital (Tainan, Taiwan). Analysis of the Oncomine cancer microarray database revealed that CCND1 gene expression was significantly increased in gastric intestinal-type adenocarcinoma, while CCND2 was significantly increased in diffuse gastric adenocarcinoma, gastric intestinal-type adenocarcinoma and gastric mixed adenocarcinoma. Kaplan-Meier analysis indicated that overexpression of CCND1 was associated with reduced OS and PFS. In addition, overexpression of CCND1 and downregulation of CCND2 were significantly correlated with receptor tyrosine-protein kinase erb-2-negative tumors and poor differentiation. The ratio of relative CCND1 expression (expressed as the CCND1/β-actin ratio) in tumor tissues compared with that in normal tissues was correlated with poor differentiation (P=0.0018). In summary, CCND1 overexpression is associated with shorter survival in patients with gastric cancer and with poorly differentiated tumors.",
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