Current status of dengue diagnosis at the center for disease control, Taiwan

Pei Yun Shu, Shu Fen Chang, Yi Yun Yueh, Ling Chow, Li Jung Chien, Yu Chung Kuo, Chien Lin Su, Tsai Ling Liao, Ting Hsiang Lin, Jyh Hsiung Huang

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

A national-level diagnostic laboratory has been set up in Taiwan for routine diagnosis of reported cases of dengue fever (DF)/dengue haemorrhagic fever (DHF), Japanese encephalitis (JE) and yellow fever (YF). The facilities include serological diagnosis, virus isolation by cell culture, molecular diagnosis and molecular tools for epidemiological investigations. To detect and differentiate dengue, JE and YF virus infections, a differential diagnostic system has been developed. For acute-phase sera, virus isolation by cell culture and real-time one-step reverse transcription-polymerase chain reaction (RT-PCR) has been established. For all of the serum samples reported, serological diagnosis of specific antibodies based on envelope and membrane (E/M)-specific capture IgM and IgG enzyme-linked immunosorbent assay (ELISA) are performed. In this report, a case study from Taiwan has been presented with the analysis of 959 serum samples (including some paired sera) collected between day 1-30 of illness from 799 confirmed dengue cases reported in 2002. The results demonstrated that 94.5% of acute-phase serum samples of confirmed dengue cases could be identified as positive or probable with the combined use of real-time one-step RT-PCR and E/M-specific capture IgM and IgG ELISA. Furthermore, a nonstructural protein NS1 serotype-specific indirect IgG ELISA has been developed and used to analyse dengue NS1-specific IgG antibodies. Both E/M-specific capture IgM and IgG ELISA and the NS1 serotype-specific indirect IgG ELISA have been used to detect and differentiate primary and secondary dengue virus infections. In addition, the NS1 serotype-specific indirect IgG ELISA has the potential of replacing the plaque-reduction neutralization test (PRNT) and is being used for a large-scale seroepidemiological study.

Original languageEnglish
Pages (from-to)107-117
Number of pages11
JournalDengue Bulletin
Volume28
Publication statusPublished - Dec 1 2004
Externally publishedYes

Fingerprint

Dengue
Centers for Disease Control and Prevention (U.S.)
Taiwan
Immunoglobulin G
Enzyme-Linked Immunosorbent Assay
Japanese Encephalitis
Immunoglobulin M
Serum
Virus Diseases
Reverse Transcription
Membranes
Cell Culture Techniques
Yellow fever virus
Viruses
Severe Dengue
Yellow Fever
Neutralization Tests
Polymerase Chain Reaction
Dengue Virus
Antibodies

Keywords

  • Dengue virus
  • E/M-specific capture IgM and IgG ELISA
  • NS1 serotype-specific indirect IgG ELISA
  • Real-time one-step RT-PCR
  • Virus isolation

ASJC Scopus subject areas

  • Infectious Diseases
  • Virology

Cite this

Shu, P. Y., Chang, S. F., Yueh, Y. Y., Chow, L., Chien, L. J., Kuo, Y. C., ... Huang, J. H. (2004). Current status of dengue diagnosis at the center for disease control, Taiwan. Dengue Bulletin, 28, 107-117.

Current status of dengue diagnosis at the center for disease control, Taiwan. / Shu, Pei Yun; Chang, Shu Fen; Yueh, Yi Yun; Chow, Ling; Chien, Li Jung; Kuo, Yu Chung; Su, Chien Lin; Liao, Tsai Ling; Lin, Ting Hsiang; Huang, Jyh Hsiung.

In: Dengue Bulletin, Vol. 28, 01.12.2004, p. 107-117.

Research output: Contribution to journalArticle

Shu, PY, Chang, SF, Yueh, YY, Chow, L, Chien, LJ, Kuo, YC, Su, CL, Liao, TL, Lin, TH & Huang, JH 2004, 'Current status of dengue diagnosis at the center for disease control, Taiwan', Dengue Bulletin, vol. 28, pp. 107-117.
Shu PY, Chang SF, Yueh YY, Chow L, Chien LJ, Kuo YC et al. Current status of dengue diagnosis at the center for disease control, Taiwan. Dengue Bulletin. 2004 Dec 1;28:107-117.
Shu, Pei Yun ; Chang, Shu Fen ; Yueh, Yi Yun ; Chow, Ling ; Chien, Li Jung ; Kuo, Yu Chung ; Su, Chien Lin ; Liao, Tsai Ling ; Lin, Ting Hsiang ; Huang, Jyh Hsiung. / Current status of dengue diagnosis at the center for disease control, Taiwan. In: Dengue Bulletin. 2004 ; Vol. 28. pp. 107-117.
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