Curcumin synergistically enhances the radiosensitivity of human oral squamous cell carcinoma via suppression of radiation-induced NF-κB activity

I. Tsang Chiang, Yu Chang Liu, Fei Ting Hsu, Yi Chun Chien, Chih Hao K Kao, Wuu Jyh Lin, Jing Gung Chung, Jeng Jong Hwang

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

The anticancer effect of curcumin has been widely reported. However, whether curcumin can enhance the radiosensitivity of human oral squamous cell carcinoma (OSCC) remains to be elucidated. The aim of the present study was to evaluate the efficacy of curcumin combined with radiation against OSCC. SAS cells were transfected with the luciferase gene (luc) and named SAS/luc. NF-κB/DNA binding activity, the surviving fraction and NF-κB-regulated effector protein expression were determined by electrophoretic mobility shift assay, clonogenic survival assay and western blotting, respectively. The therapeutic efficacy was evaluated in SAS/luc tumor-bearing mice by caliper measurement and bioluminescence imaging. Curcumin enhanced SAS/luc radiosensitivity through the inhibition of radiation-induced NF-κB activity and expression of effector proteins both in vitro and in vivo. With 4 Gy or greater radiation doses, synergistic effects of curcumin were observed. The combination group (curcumin plus radiation) had significantly better tumor control compared with that of curcumin or radiation alone. No significant body weight change of mice was found throughout the entire study. In conclusion, curcumin is a radiosensitizer against OSCC with negligible toxicity.

Original languageEnglish
Pages (from-to)1729-1737
Number of pages9
JournalOncology Reports
Volume31
Issue number4
DOIs
Publication statusPublished - 2014
Externally publishedYes

Fingerprint

Curcumin
Radiation Tolerance
Squamous Cell Carcinoma
Radiation
Luminescent Measurements
Body Weight Changes
Electrophoretic Mobility Shift Assay
Luciferases
Neoplasms
Proteins
Western Blotting
Survival
DNA

Keywords

  • Curcumin
  • Nuclear factor-κB
  • Radiotherapy

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Curcumin synergistically enhances the radiosensitivity of human oral squamous cell carcinoma via suppression of radiation-induced NF-κB activity. / Chiang, I. Tsang; Liu, Yu Chang; Hsu, Fei Ting; Chien, Yi Chun; Kao, Chih Hao K; Lin, Wuu Jyh; Chung, Jing Gung; Hwang, Jeng Jong.

In: Oncology Reports, Vol. 31, No. 4, 2014, p. 1729-1737.

Research output: Contribution to journalArticle

Chiang, I. Tsang ; Liu, Yu Chang ; Hsu, Fei Ting ; Chien, Yi Chun ; Kao, Chih Hao K ; Lin, Wuu Jyh ; Chung, Jing Gung ; Hwang, Jeng Jong. / Curcumin synergistically enhances the radiosensitivity of human oral squamous cell carcinoma via suppression of radiation-induced NF-κB activity. In: Oncology Reports. 2014 ; Vol. 31, No. 4. pp. 1729-1737.
@article{38da70cfd8e0483cb09d2bfc1dca9b67,
title = "Curcumin synergistically enhances the radiosensitivity of human oral squamous cell carcinoma via suppression of radiation-induced NF-κB activity",
abstract = "The anticancer effect of curcumin has been widely reported. However, whether curcumin can enhance the radiosensitivity of human oral squamous cell carcinoma (OSCC) remains to be elucidated. The aim of the present study was to evaluate the efficacy of curcumin combined with radiation against OSCC. SAS cells were transfected with the luciferase gene (luc) and named SAS/luc. NF-κB/DNA binding activity, the surviving fraction and NF-κB-regulated effector protein expression were determined by electrophoretic mobility shift assay, clonogenic survival assay and western blotting, respectively. The therapeutic efficacy was evaluated in SAS/luc tumor-bearing mice by caliper measurement and bioluminescence imaging. Curcumin enhanced SAS/luc radiosensitivity through the inhibition of radiation-induced NF-κB activity and expression of effector proteins both in vitro and in vivo. With 4 Gy or greater radiation doses, synergistic effects of curcumin were observed. The combination group (curcumin plus radiation) had significantly better tumor control compared with that of curcumin or radiation alone. No significant body weight change of mice was found throughout the entire study. In conclusion, curcumin is a radiosensitizer against OSCC with negligible toxicity.",
keywords = "Curcumin, Nuclear factor-κB, Radiotherapy",
author = "Chiang, {I. Tsang} and Liu, {Yu Chang} and Hsu, {Fei Ting} and Chien, {Yi Chun} and Kao, {Chih Hao K} and Lin, {Wuu Jyh} and Chung, {Jing Gung} and Hwang, {Jeng Jong}",
year = "2014",
doi = "10.3892/or.2014.3009",
language = "English",
volume = "31",
pages = "1729--1737",
journal = "Oncology Reports",
issn = "1021-335X",
publisher = "Spandidos Publications",
number = "4",

}

TY - JOUR

T1 - Curcumin synergistically enhances the radiosensitivity of human oral squamous cell carcinoma via suppression of radiation-induced NF-κB activity

AU - Chiang, I. Tsang

AU - Liu, Yu Chang

AU - Hsu, Fei Ting

AU - Chien, Yi Chun

AU - Kao, Chih Hao K

AU - Lin, Wuu Jyh

AU - Chung, Jing Gung

AU - Hwang, Jeng Jong

PY - 2014

Y1 - 2014

N2 - The anticancer effect of curcumin has been widely reported. However, whether curcumin can enhance the radiosensitivity of human oral squamous cell carcinoma (OSCC) remains to be elucidated. The aim of the present study was to evaluate the efficacy of curcumin combined with radiation against OSCC. SAS cells were transfected with the luciferase gene (luc) and named SAS/luc. NF-κB/DNA binding activity, the surviving fraction and NF-κB-regulated effector protein expression were determined by electrophoretic mobility shift assay, clonogenic survival assay and western blotting, respectively. The therapeutic efficacy was evaluated in SAS/luc tumor-bearing mice by caliper measurement and bioluminescence imaging. Curcumin enhanced SAS/luc radiosensitivity through the inhibition of radiation-induced NF-κB activity and expression of effector proteins both in vitro and in vivo. With 4 Gy or greater radiation doses, synergistic effects of curcumin were observed. The combination group (curcumin plus radiation) had significantly better tumor control compared with that of curcumin or radiation alone. No significant body weight change of mice was found throughout the entire study. In conclusion, curcumin is a radiosensitizer against OSCC with negligible toxicity.

AB - The anticancer effect of curcumin has been widely reported. However, whether curcumin can enhance the radiosensitivity of human oral squamous cell carcinoma (OSCC) remains to be elucidated. The aim of the present study was to evaluate the efficacy of curcumin combined with radiation against OSCC. SAS cells were transfected with the luciferase gene (luc) and named SAS/luc. NF-κB/DNA binding activity, the surviving fraction and NF-κB-regulated effector protein expression were determined by electrophoretic mobility shift assay, clonogenic survival assay and western blotting, respectively. The therapeutic efficacy was evaluated in SAS/luc tumor-bearing mice by caliper measurement and bioluminescence imaging. Curcumin enhanced SAS/luc radiosensitivity through the inhibition of radiation-induced NF-κB activity and expression of effector proteins both in vitro and in vivo. With 4 Gy or greater radiation doses, synergistic effects of curcumin were observed. The combination group (curcumin plus radiation) had significantly better tumor control compared with that of curcumin or radiation alone. No significant body weight change of mice was found throughout the entire study. In conclusion, curcumin is a radiosensitizer against OSCC with negligible toxicity.

KW - Curcumin

KW - Nuclear factor-κB

KW - Radiotherapy

UR - http://www.scopus.com/inward/record.url?scp=84908140616&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84908140616&partnerID=8YFLogxK

U2 - 10.3892/or.2014.3009

DO - 10.3892/or.2014.3009

M3 - Article

C2 - 24503718

AN - SCOPUS:84908140616

VL - 31

SP - 1729

EP - 1737

JO - Oncology Reports

JF - Oncology Reports

SN - 1021-335X

IS - 4

ER -