Curcumin Supplementation Ameliorated Vascular Dysfunction and Antioxidant Status in High Sucrose, High Fat Fed Rats

I-Jung Tsai, Chia-Wen Chen, Shin-Yu Tsai, Pei-Yuan Wang, Eddy Owaga, Rong-Hong Hsieh

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Abstract

Vascular endothelial dysfunction is a potential risk factor for cardiovascular diseases. This study evaluated the effect of curcumin on vascular dysfunction relative factors using rats fed on high sucrose, high fat (HSF) diet. The experiment was conducted in two animal feeding stages. In the first feeding phase, male Sprague-Dawley rats were randomly divided into two groups: control group (n=8) was fed on AIN-93G diet and HSF group (n=24) was fed on HSF diet for 8 weeks to induce obese status. In the second feeding phase lasting 4 weeks, HSF group was further randomly subdivided into three subgroups: O group (n=8) continued feeding on HSF diet; OA group (n=8) had HSF diet replaced with AIN-93G diet; and OC group (n=8) was fed on HSF diet supplemented with curcumin (300mg/kg body weight/day). After 8 weeks, HSF diet significantly elevated levels of AST, ALT, insulin, HOMA-IR, LDL-C, Hcy, CRP, VCAM-1 and ICAM-1, but significantly reduced levels of NO and HDL-C. After dietary intervention, OA and OC groups exhibited significantly lower levels of AST, ALT, HOMA-IR, cholesterol, LDL-C, Hcy, CRP, VCAM-1 and ICAM-1, and higher levels of NO and CAT activity compared to those of O group. SOD, CAT and GPx activities were increased in OA group. CAT levels were enhanced in OC group. In conclusion, this study showed curcumin supplementation and dietary replacement can inhibit HSF diet-induced vascular dysfunction potentially through enhanced NO production and antioxidant enzymes activities, thereby leading to suppressed inflammation and oxidative damage in the vascular endothelium.

Original languageEnglish
JournalApplied Physiology, Nutrition and Metabolism
DOIs
Publication statusE-pub ahead of print - Jan 29 2018

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Curcumin
Blood Vessels
Sucrose
High Fat Diet
Antioxidants
Fats
Vascular Cell Adhesion Molecule-1
Intercellular Adhesion Molecule-1
Diet
Vascular Endothelium
Dietary Supplements
LDL Cholesterol
Sprague Dawley Rats
Cardiovascular Diseases
Body Weight
Insulin
Inflammation
Control Groups

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Curcumin Supplementation Ameliorated Vascular Dysfunction and Antioxidant Status in High Sucrose, High Fat Fed Rats. / Tsai, I-Jung; Chen, Chia-Wen; Tsai, Shin-Yu; Wang, Pei-Yuan; Owaga, Eddy; Hsieh, Rong-Hong.

In: Applied Physiology, Nutrition and Metabolism, 29.01.2018.

Research output: Contribution to journalArticle

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