Curcumin attenuates the expression and secretion of RANTES after spinal cord injury in vivo and lipopolysaccharide-induced astrocyte reactivation in vitro

Muh Shi Lin, Yu Yo Sun, Wen Ta Chiu, Chia Chi Hung, Chiu Yun Chang, Feng Shiun Shie, Shin Han Tsai, Jia Wei Lin, Kuo Sheng Hung, Yi Hsuan Lee

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Curcumin has been proposed for treatment of various neuroinflammatory and neurodegenerative conditions, including post-traumatic inflammation during acute spinal cord injury (SCI). In this study, we examined whether curcumin anti-inflammation involves regulation of astrocyte reactivation, with special focus on the injury-induced RANTES (regulated on expression normal T-cell expressed and secreted) from astrocytes in acute SCI. Male Sprague-Dawley (SD) rats were subjected to impact injury of the spinal cord followed by treatment with curcumin (40mg/kg i.p.). RANTES and inducible nitric oxide synthase expression as well as RANTES-positive astrocytes were all induced by injury accompanied by the elevation of lipid peroxidation, and attenuated by the curcumin treatment. In primary cultured rat astrocytes challenged with lipopolysaccharide (LPS) to mimic astrocyte reactivation following SCI, LPS induces robust increase of RANTES expression and the effect was also reduced by 1?M curcumin treatment. Furthermore, cortical neurons cultured with astrocyte conditioned medium (ACM) conditioned with both LPS and curcumin (LPS-curcumin/ACM), which characteristically exhibited decreased RANTES expression when compared with ACM from astrocytes treated with LPS alone (LPS/ACM), showed higher level of cell viability and lower level of cell death as assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reduction activity assay and lactate dehydrogenase release assay, respectively. Knockdown of RANTES expression by siRNA (siRANTES) shows reduced RANTES expression and release from LPS-reactivated astrocytes, and ACM obtained from this condition (LPS-siRANTES/ACM) becomes less cytotoxic as compared with the LPS-ACM. Therefore, curcumin reduction of robust RANTES production in reactivated astrocytes both in vitro and in vivo may contribute to its neuroprotection and potential application in SCI.

Original languageEnglish
Pages (from-to)1259-1269
Number of pages11
JournalJournal of Neurotrauma
Volume28
Issue number7
DOIs
Publication statusPublished - Jul 1 2011

Fingerprint

Curcumin
Spinal Cord Injuries
Astrocytes
Lipopolysaccharides
T-Lymphocytes
Conditioned Culture Medium
In Vitro Techniques
Inflammation
Wounds and Injuries
Nitric Oxide Synthase Type II
L-Lactate Dehydrogenase
Small Interfering RNA
Lipid Peroxidation

Keywords

  • astrocyte
  • curcumin
  • iNOS
  • neuroprotection
  • RANTES
  • SCI

ASJC Scopus subject areas

  • Clinical Neurology

Cite this

Curcumin attenuates the expression and secretion of RANTES after spinal cord injury in vivo and lipopolysaccharide-induced astrocyte reactivation in vitro. / Lin, Muh Shi; Sun, Yu Yo; Chiu, Wen Ta; Hung, Chia Chi; Chang, Chiu Yun; Shie, Feng Shiun; Tsai, Shin Han; Lin, Jia Wei; Hung, Kuo Sheng; Lee, Yi Hsuan.

In: Journal of Neurotrauma, Vol. 28, No. 7, 01.07.2011, p. 1259-1269.

Research output: Contribution to journalArticle

Lin, Muh Shi ; Sun, Yu Yo ; Chiu, Wen Ta ; Hung, Chia Chi ; Chang, Chiu Yun ; Shie, Feng Shiun ; Tsai, Shin Han ; Lin, Jia Wei ; Hung, Kuo Sheng ; Lee, Yi Hsuan. / Curcumin attenuates the expression and secretion of RANTES after spinal cord injury in vivo and lipopolysaccharide-induced astrocyte reactivation in vitro. In: Journal of Neurotrauma. 2011 ; Vol. 28, No. 7. pp. 1259-1269.
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