Abstract

Curcumin and saikosaponin A as antioxidants improve antioxidant status. This study investigated the anti-inflammatory and antifibrotic actions of curcumin and saikosaponin A on CCl4-induced liver damage. Sprague-Dawley rats were randomly divided into control, CCl4, CCl4+ curcumin (0.005%; CU), CCl4 + saikosaponin A (0.004%; SS), and CCl4 + curcumin + saikosaponin A (0.005% + 0.004%; CU + SS) groups. Carbon tetrachloride (40% in olive oil) at a dose of 0.75 ml/kg was injected intraperitoneally once a week. Curcumin and saikosaponin A were supplemented alone or in combination with diet 1 week before CCl4 injection for 8 weeks. After 8-week supplementation, histopathological results showed hepatic collagen deposition was significantly reduced in the CU and SS groups, and activated nuclear factor-κ B expression induced by CCl 4 in the liver was significantly inhibited by curcumin and/or saikosaponin A. Hepatic proinflammatory cytokines tumor necrosis factor-α, interleukin-1β, and interleukin-6 were significantly inhibited, and anti-inflammatory cytokine interleukin-10 was significantly increased by supplementation with curcumin and/or saikosaponin A. Additionally, curcumin and/or saikosaponin A significantly reduced the increased levels of hepatic transforming growth factor-β1 and hydroxyproline after CCl4 treatment. Therefore, supplementation with curcumin and/or saikosaponin A suppress inflammation and fibrogenesis in rats with CCl4-induced liver injury. However, the combination has no additive effects on anti-inflammation and antifibrosis.

Original languageEnglish
Pages (from-to)99-111
Number of pages13
JournalAmerican Journal of Chinese Medicine
Volume38
Issue number1
DOIs
Publication statusPublished - 2010

Fingerprint

Curcumin
Liver Cirrhosis
Inflammation
Liver
Anti-Inflammatory Agents
Antioxidants
Cytokines
saikosaponin
Carbon Tetrachloride
Hydroxyproline
Transforming Growth Factors
Interleukin-1
Interleukin-10
Sprague Dawley Rats
Interleukin-6
Collagen
Tumor Necrosis Factor-alpha
Diet
Injections
Wounds and Injuries

Keywords

  • CCl
  • Curcumin
  • Liver fibrosis
  • Liver inflammation
  • Rats
  • Saikosaponin A

ASJC Scopus subject areas

  • Complementary and alternative medicine

Cite this

Curcumin and saikosaponin A inhibit chemical-induced liver inflammation and fibrosis in rats. / Wu, Shu Ju; Tam, Ka Wai; Tsai, Ya Hui; Chang, Chun Chao; Chao, Jane C J.

In: American Journal of Chinese Medicine, Vol. 38, No. 1, 2010, p. 99-111.

Research output: Contribution to journalArticle

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abstract = "Curcumin and saikosaponin A as antioxidants improve antioxidant status. This study investigated the anti-inflammatory and antifibrotic actions of curcumin and saikosaponin A on CCl4-induced liver damage. Sprague-Dawley rats were randomly divided into control, CCl4, CCl4+ curcumin (0.005{\%}; CU), CCl4 + saikosaponin A (0.004{\%}; SS), and CCl4 + curcumin + saikosaponin A (0.005{\%} + 0.004{\%}; CU + SS) groups. Carbon tetrachloride (40{\%} in olive oil) at a dose of 0.75 ml/kg was injected intraperitoneally once a week. Curcumin and saikosaponin A were supplemented alone or in combination with diet 1 week before CCl4 injection for 8 weeks. After 8-week supplementation, histopathological results showed hepatic collagen deposition was significantly reduced in the CU and SS groups, and activated nuclear factor-κ B expression induced by CCl 4 in the liver was significantly inhibited by curcumin and/or saikosaponin A. Hepatic proinflammatory cytokines tumor necrosis factor-α, interleukin-1β, and interleukin-6 were significantly inhibited, and anti-inflammatory cytokine interleukin-10 was significantly increased by supplementation with curcumin and/or saikosaponin A. Additionally, curcumin and/or saikosaponin A significantly reduced the increased levels of hepatic transforming growth factor-β1 and hydroxyproline after CCl4 treatment. Therefore, supplementation with curcumin and/or saikosaponin A suppress inflammation and fibrogenesis in rats with CCl4-induced liver injury. However, the combination has no additive effects on anti-inflammation and antifibrosis.",
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