Cuphiin D1, the macrocyclic hydrolyzable tannin induced apoptosis in HL-60 cell line

Ching Chiung Wang, Lih Geeng Chen, Ling Ling Yang

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Cuphiin D1 (CD1), a new macrocyclic hydrolyzable tannin isolated from Cuphea hyssopifolia, has been shown to exert antitumor activity both in vitro and in vivo. In this study, we explored the mechanism of the CD1-induced antitumor effect on human promyelocytic leukemia (HL-60) cells. The results showed that CD1 induced cytotoxicity in HL-60 cells and the IC50 was 16 μM after 36 h treatment. HL-60 cells treated with CD1 for 36 h decreased the uptake of [3H]-labeled thymidine, uridine and leucine in a dose dependent manner. Electron micrographs demonstrated that HL-60 cells treated with 16 μM CD1 for 36 h exhibited chromatin condensation, indicating the apoptosis occurrence. Flow cytometric analysis demonstrated the presence of apoptotic cells with low DNA content, a decrease of cell population at G2/M phase, and a concomitant increase of cell population at G1 phase. CD1 also caused DNA fragmentation and inhibited Bcl-2 expression in the HL-60 cells. These results suggest that the inhibition of Bcl-2 expression in HL-60 cell might account for the mechanism of CD1-induced apoptosis. (C) 2000 Elsevier Science Ireland Ltd.

Original languageEnglish
Pages (from-to)77-83
Number of pages7
JournalCancer Letters
Volume149
Issue number1-2
DOIs
Publication statusPublished - Feb 28 2000
Externally publishedYes

Fingerprint

Hydrolyzable Tannins
HL-60 Cells
Apoptosis
Cell Line
Cuphea
G2 Phase
Uridine
G1 Phase
Population Dynamics
DNA Fragmentation
cuphiin D(1)
Leucine
Cell Division
Thymidine
Inhibitory Concentration 50
Chromatin
Leukemia
Electrons
DNA

Keywords

  • Apoptosis
  • Bcl-2
  • Cuphea hyssopifolia
  • Cuphiin D
  • HL-60 cells
  • Tannin

ASJC Scopus subject areas

  • Cancer Research
  • Molecular Biology
  • Oncology

Cite this

Cuphiin D1, the macrocyclic hydrolyzable tannin induced apoptosis in HL-60 cell line. / Wang, Ching Chiung; Chen, Lih Geeng; Yang, Ling Ling.

In: Cancer Letters, Vol. 149, No. 1-2, 28.02.2000, p. 77-83.

Research output: Contribution to journalArticle

Wang, Ching Chiung ; Chen, Lih Geeng ; Yang, Ling Ling. / Cuphiin D1, the macrocyclic hydrolyzable tannin induced apoptosis in HL-60 cell line. In: Cancer Letters. 2000 ; Vol. 149, No. 1-2. pp. 77-83.
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