CSE1L modulates ras-induced cancer cell invasion: Correlation of K-ras mutation and CSE1L expression in colorectal cancer progression

Ming Chung Jiang, Chung Min Yeh, Cheng Jeng Tai, Hung Chang Chen, Shu Hui Lin, Tzu Cheng Su, Shing Chuan Shen, Woan Ruoh Lee, Ching Fong Liao, Li Tzu Li, Ching Hsiao Lee, Ying Chun Chen, Kun Tu Yeh, Chun Chao Chang

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Background: Ras plays an important role in colorectal cancer progression. CSE1L (chromosome segregation 1-like) gene maps to 20q13, a chromosomal region that correlates with colorectal cancer development. We investigated the association of CSE1L with Ras in colorectal cancer progression. Methods: The effect of CSE1L on metastasis-stimulating activity of Ras was studied in an animal model with tumor cells expressing CSE1L-specific shRNA and v-H-Ras. CSE1L expression was evaluated by the immunohistochemical analysis of 127 surgically resected colorectal tumors. K-Ras mutations were analyzed by direct sequencing. Results: CSE1L knockdown reduced Ras-induced metastasis of B16F10 melanoma cells in C57BL/6 mice. v-H-Ras expression altered the cellular trafficking of CSE1L and increased CSE1L secretion. Most colorectal tumors were positive for CSE1L staining (98.4%, 125 of 127). Colorectal tumors with K-Ras mutation or high cytoplasmic CSE1L expression were correlated with T status (depth of tumor penetration; P =.004), stage (P =.004), and lymph node metastasis (P =.019). Conclusions: CSE1L may be a target for treating Ras-associated tumors. Analysis of K-Ras mutation and CSE1L expression may provide valuable clinical and pathological information to aid in the determination of treatment options for colorectal cancer.

Original languageEnglish
Pages (from-to)418-427
Number of pages10
JournalAmerican Journal of Surgery
Volume206
Issue number3
DOIs
Publication statusPublished - Sep 2013

Fingerprint

Chromosome Segregation
Chromosomes, Human, Pair 1
Colorectal Neoplasms
Mutation
Neoplasms
Neoplasm Metastasis
Inbred C57BL Mouse
Small Interfering RNA
Melanoma
Animal Models
Lymph Nodes

Keywords

  • Colorectal cancer
  • CSE1L
  • K-Ras
  • Lymph node
  • Metastasis

ASJC Scopus subject areas

  • Surgery

Cite this

CSE1L modulates ras-induced cancer cell invasion : Correlation of K-ras mutation and CSE1L expression in colorectal cancer progression. / Jiang, Ming Chung; Yeh, Chung Min; Tai, Cheng Jeng; Chen, Hung Chang; Lin, Shu Hui; Su, Tzu Cheng; Shen, Shing Chuan; Lee, Woan Ruoh; Liao, Ching Fong; Li, Li Tzu; Lee, Ching Hsiao; Chen, Ying Chun; Yeh, Kun Tu; Chang, Chun Chao.

In: American Journal of Surgery, Vol. 206, No. 3, 09.2013, p. 418-427.

Research output: Contribution to journalArticle

Jiang, Ming Chung ; Yeh, Chung Min ; Tai, Cheng Jeng ; Chen, Hung Chang ; Lin, Shu Hui ; Su, Tzu Cheng ; Shen, Shing Chuan ; Lee, Woan Ruoh ; Liao, Ching Fong ; Li, Li Tzu ; Lee, Ching Hsiao ; Chen, Ying Chun ; Yeh, Kun Tu ; Chang, Chun Chao. / CSE1L modulates ras-induced cancer cell invasion : Correlation of K-ras mutation and CSE1L expression in colorectal cancer progression. In: American Journal of Surgery. 2013 ; Vol. 206, No. 3. pp. 418-427.
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abstract = "Background: Ras plays an important role in colorectal cancer progression. CSE1L (chromosome segregation 1-like) gene maps to 20q13, a chromosomal region that correlates with colorectal cancer development. We investigated the association of CSE1L with Ras in colorectal cancer progression. Methods: The effect of CSE1L on metastasis-stimulating activity of Ras was studied in an animal model with tumor cells expressing CSE1L-specific shRNA and v-H-Ras. CSE1L expression was evaluated by the immunohistochemical analysis of 127 surgically resected colorectal tumors. K-Ras mutations were analyzed by direct sequencing. Results: CSE1L knockdown reduced Ras-induced metastasis of B16F10 melanoma cells in C57BL/6 mice. v-H-Ras expression altered the cellular trafficking of CSE1L and increased CSE1L secretion. Most colorectal tumors were positive for CSE1L staining (98.4{\%}, 125 of 127). Colorectal tumors with K-Ras mutation or high cytoplasmic CSE1L expression were correlated with T status (depth of tumor penetration; P =.004), stage (P =.004), and lymph node metastasis (P =.019). Conclusions: CSE1L may be a target for treating Ras-associated tumors. Analysis of K-Ras mutation and CSE1L expression may provide valuable clinical and pathological information to aid in the determination of treatment options for colorectal cancer.",
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T1 - CSE1L modulates ras-induced cancer cell invasion

T2 - Correlation of K-ras mutation and CSE1L expression in colorectal cancer progression

AU - Jiang, Ming Chung

AU - Yeh, Chung Min

AU - Tai, Cheng Jeng

AU - Chen, Hung Chang

AU - Lin, Shu Hui

AU - Su, Tzu Cheng

AU - Shen, Shing Chuan

AU - Lee, Woan Ruoh

AU - Liao, Ching Fong

AU - Li, Li Tzu

AU - Lee, Ching Hsiao

AU - Chen, Ying Chun

AU - Yeh, Kun Tu

AU - Chang, Chun Chao

PY - 2013/9

Y1 - 2013/9

N2 - Background: Ras plays an important role in colorectal cancer progression. CSE1L (chromosome segregation 1-like) gene maps to 20q13, a chromosomal region that correlates with colorectal cancer development. We investigated the association of CSE1L with Ras in colorectal cancer progression. Methods: The effect of CSE1L on metastasis-stimulating activity of Ras was studied in an animal model with tumor cells expressing CSE1L-specific shRNA and v-H-Ras. CSE1L expression was evaluated by the immunohistochemical analysis of 127 surgically resected colorectal tumors. K-Ras mutations were analyzed by direct sequencing. Results: CSE1L knockdown reduced Ras-induced metastasis of B16F10 melanoma cells in C57BL/6 mice. v-H-Ras expression altered the cellular trafficking of CSE1L and increased CSE1L secretion. Most colorectal tumors were positive for CSE1L staining (98.4%, 125 of 127). Colorectal tumors with K-Ras mutation or high cytoplasmic CSE1L expression were correlated with T status (depth of tumor penetration; P =.004), stage (P =.004), and lymph node metastasis (P =.019). Conclusions: CSE1L may be a target for treating Ras-associated tumors. Analysis of K-Ras mutation and CSE1L expression may provide valuable clinical and pathological information to aid in the determination of treatment options for colorectal cancer.

AB - Background: Ras plays an important role in colorectal cancer progression. CSE1L (chromosome segregation 1-like) gene maps to 20q13, a chromosomal region that correlates with colorectal cancer development. We investigated the association of CSE1L with Ras in colorectal cancer progression. Methods: The effect of CSE1L on metastasis-stimulating activity of Ras was studied in an animal model with tumor cells expressing CSE1L-specific shRNA and v-H-Ras. CSE1L expression was evaluated by the immunohistochemical analysis of 127 surgically resected colorectal tumors. K-Ras mutations were analyzed by direct sequencing. Results: CSE1L knockdown reduced Ras-induced metastasis of B16F10 melanoma cells in C57BL/6 mice. v-H-Ras expression altered the cellular trafficking of CSE1L and increased CSE1L secretion. Most colorectal tumors were positive for CSE1L staining (98.4%, 125 of 127). Colorectal tumors with K-Ras mutation or high cytoplasmic CSE1L expression were correlated with T status (depth of tumor penetration; P =.004), stage (P =.004), and lymph node metastasis (P =.019). Conclusions: CSE1L may be a target for treating Ras-associated tumors. Analysis of K-Ras mutation and CSE1L expression may provide valuable clinical and pathological information to aid in the determination of treatment options for colorectal cancer.

KW - Colorectal cancer

KW - CSE1L

KW - K-Ras

KW - Lymph node

KW - Metastasis

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