CSE1L, a novel microvesicle membrane protein, mediates Ras-triggered microvesicle generation and metastasis of tumor cells

Ching Fong Liao, Shu Hui Lin, Hung Chang Chen, Cheng Jeng Tai, Chun Chao Chang, Li Tzu Li, Chung Min Yeh, Kun Tu Yeh, Ying Chun Chen, Tsu Han Hsu, Shing Chuan Shen, Woan Ruoh Lee, Jeng Fong Chiou, Shue Fen Luo, Ming Chung Jiang

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Tumor-derived microvesicles are rich in metastasis-related proteases and play a role in the interactions between tumor cells and tumor microenvironment in tumor metastasis. Because shed microvesicles may remain in the extracellular environment around tumor cells, the microvesicle membrane protein may be the potential target for cancer therapy. Here we report that chromosome segregation 1-like (CSE1L) protein is a microvesicle membrane protein and is a potential target for cancer therapy. v-H-Ras expression induced extracellular signal-regulated kinase (ERK)-dependent CSE1L phosphorylation and microvesicle biogenesis in various cancer cells. CSE1L overexpression also triggered microvesicle generation, and CSE1L knockdown diminished v-H-Ras-induced microvesicle generation, matrix metalloproteinase (MMP)-2 and MMP-9 secretion and metastasis of B16F10 melanoma cells. CSE1L was preferentially accumulated in microvesicles and was located in the microvesicle membrane. Furthermore, anti-CSE1L antibody-conjugated quantum dots could target tumors in animal models. Our findings highlight a novel role of Ras-ERK signaling in tumor progression and suggest that CSE1L may be involved in the "early" and "late" metastasis of tumor cells in tumorigenesis. Furthermore, the novel microvesicle membrane protein, CSE1L, may have clinical utility in cancer diagnosis and targeted cancer therapy.

Original languageEnglish
Pages (from-to)1269-1280
Number of pages12
JournalMolecular Medicine
Volume18
Issue number9
DOIs
Publication statusPublished - 2012

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Chromosome Segregation
Chromosomes, Human, Pair 1
Membrane Proteins
Neoplasm Metastasis
Neoplasms
Extracellular Signal-Regulated MAP Kinases
Cellular Apoptosis Susceptibility Protein
Cellular Microenvironment
Quantum Dots
Tumor Microenvironment
Matrix Metalloproteinase 2
Matrix Metalloproteinase 9
Melanoma
Carcinogenesis
Peptide Hydrolases
Therapeutics
Animal Models

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Molecular Medicine
  • Genetics(clinical)

Cite this

CSE1L, a novel microvesicle membrane protein, mediates Ras-triggered microvesicle generation and metastasis of tumor cells. / Liao, Ching Fong; Lin, Shu Hui; Chen, Hung Chang; Tai, Cheng Jeng; Chang, Chun Chao; Li, Li Tzu; Yeh, Chung Min; Yeh, Kun Tu; Chen, Ying Chun; Hsu, Tsu Han; Shen, Shing Chuan; Lee, Woan Ruoh; Chiou, Jeng Fong; Luo, Shue Fen; Jiang, Ming Chung.

In: Molecular Medicine, Vol. 18, No. 9, 2012, p. 1269-1280.

Research output: Contribution to journalArticle

Liao, Ching Fong ; Lin, Shu Hui ; Chen, Hung Chang ; Tai, Cheng Jeng ; Chang, Chun Chao ; Li, Li Tzu ; Yeh, Chung Min ; Yeh, Kun Tu ; Chen, Ying Chun ; Hsu, Tsu Han ; Shen, Shing Chuan ; Lee, Woan Ruoh ; Chiou, Jeng Fong ; Luo, Shue Fen ; Jiang, Ming Chung. / CSE1L, a novel microvesicle membrane protein, mediates Ras-triggered microvesicle generation and metastasis of tumor cells. In: Molecular Medicine. 2012 ; Vol. 18, No. 9. pp. 1269-1280.
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