Crystallization and preliminary X-ray diffraction characterization of RpfF, a key DSF synthase from Stenotrophomonas maltophilia

Xian Ya Lin, Shu Ju Liao, Ko Hsin Chin, Hui Ling Shih, Gwan Han Shen, Andrew H.J. Wang, Shan Ho Chou

Research output: Contribution to journalArticlepeer-review

Abstract

Stenotrophomonas maltophilia has emerged as a critical nosocomial opportunistic pathogen in the last few years. It is resistant to many clinically useful antibiotics; hence, new ways of combatting this bacterium are essential. Diffusible signal factor (DSF) dependent quorum sensing is a major mechanism of virulence induction in S. maltophilia, with RpfF playing a key role in DSF biosynthesis. Inhibiting S. maltophilia RpfF (SmRpfF) function via small-molecule interference may constitute a new way of treating S. maltophilia infection. SmRpfF was therefore overexpressed in Escherichia coli, purified and crystallized using the hanging-drop vapour-diffusion method. The crystals belonged to the tetragonal space group P41212 or P43212, with unit-cell parameters a = b = 148.51, c = 122.82 Å, and diffracted to a resolution of 2.25 Å.

Original languageEnglish
Pages (from-to)1021-1023
Number of pages3
JournalActa Crystallographica Section F: Structural Biology and Crystallization Communications
Volume65
Issue number10
DOIs
Publication statusPublished - 2009
Externally publishedYes

Keywords

  • DFS synthase
  • RpfF
  • Stenotrophomonas maltophilia
  • Structural genomics

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Genetics
  • Condensed Matter Physics

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