Crystallization and preliminary X-ray diffraction analysis of recombinant hepatitis e virus-like particle

Che Yen Wang, Naoyuki Miyazaki, Tetsuo Yamashita, Akifumi Higashiura, Atsushi Nakagawa, Tian Cheng Li, Naokazu Takeda, Li Xing, Erik Hjalmarsson, Claes Friberg, Der Ming Liou, Yen Jen Sung, Tomitake Tsukihara, Yoshiharu Matsuura, Tatsuo Miyamura, R. Holland Cheng

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Hepatitis E virus (HEV) accounts for the majority of enterically transmitted hepatitis infections worldwide. Currently, there is no specific treatment for or vaccine against HEV. The major structural protein is derived from open reading frame (ORF) 2 of the viral genome. A potential oral vaccine is provided by the virus-like particles formed by a protein construct of partial ORF3 protein (residue 70-123) fused to the N-terminus of the ORF2 protein (residues 112-608). Single crystals obtained by the hanging-drop vapour-diffusion method at 293 K diffract X-rays to 8.3 Å resolution. The crystals belong to space group P212121, with unit-cell parameters a = 337, b = 343, c = 346 Å, α = β = γ = 90°, and contain one particle per asymmetric unit.

Original languageEnglish
Pages (from-to)318-322
Number of pages5
JournalActa Crystallographica Section F: Structural Biology and Crystallization Communications
Volume64
Issue number4
DOIs
Publication statusPublished - Mar 29 2008
Externally publishedYes

Fingerprint

hepatitis
Hepatitis Viruses
viruses
Crystallization
Viruses
X-Ray Diffraction
Virion
X ray diffraction analysis
crystallization
vaccines
Hepatitis E virus
proteins
diffraction
Proteins
x rays
Vaccines
genome
Viral Genome
infectious diseases
Hepatitis

Keywords

  • Electron microscopy
  • Hepatitis E virus
  • Vaccines
  • Virus-like particle

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Genetics
  • Condensed Matter Physics

Cite this

Crystallization and preliminary X-ray diffraction analysis of recombinant hepatitis e virus-like particle. / Wang, Che Yen; Miyazaki, Naoyuki; Yamashita, Tetsuo; Higashiura, Akifumi; Nakagawa, Atsushi; Li, Tian Cheng; Takeda, Naokazu; Xing, Li; Hjalmarsson, Erik; Friberg, Claes; Liou, Der Ming; Sung, Yen Jen; Tsukihara, Tomitake; Matsuura, Yoshiharu; Miyamura, Tatsuo; Cheng, R. Holland.

In: Acta Crystallographica Section F: Structural Biology and Crystallization Communications, Vol. 64, No. 4, 29.03.2008, p. 318-322.

Research output: Contribution to journalArticle

Wang, CY, Miyazaki, N, Yamashita, T, Higashiura, A, Nakagawa, A, Li, TC, Takeda, N, Xing, L, Hjalmarsson, E, Friberg, C, Liou, DM, Sung, YJ, Tsukihara, T, Matsuura, Y, Miyamura, T & Cheng, RH 2008, 'Crystallization and preliminary X-ray diffraction analysis of recombinant hepatitis e virus-like particle', Acta Crystallographica Section F: Structural Biology and Crystallization Communications, vol. 64, no. 4, pp. 318-322. https://doi.org/10.1107/S1744309108007197
Wang, Che Yen ; Miyazaki, Naoyuki ; Yamashita, Tetsuo ; Higashiura, Akifumi ; Nakagawa, Atsushi ; Li, Tian Cheng ; Takeda, Naokazu ; Xing, Li ; Hjalmarsson, Erik ; Friberg, Claes ; Liou, Der Ming ; Sung, Yen Jen ; Tsukihara, Tomitake ; Matsuura, Yoshiharu ; Miyamura, Tatsuo ; Cheng, R. Holland. / Crystallization and preliminary X-ray diffraction analysis of recombinant hepatitis e virus-like particle. In: Acta Crystallographica Section F: Structural Biology and Crystallization Communications. 2008 ; Vol. 64, No. 4. pp. 318-322.
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