D-Aminoacylases catalyze the hydrolysis of N-acyl-D-amino acids into D-amino acids with the aid of zinc ions. The first D-aminoacylase crystal from Alcaligenes faecalis has been obtained in hanging drops at pH 5.6 by the vapour-diffusion method using 30% polyethylene glycol 4000 as precipitant. It belongs to space group P212121, with unit-cell parameters a = 60.2, b = 76.6, c = 135.3 Å. Reflections to 1.2 Å resolution are observable. An initial atomic model with 472 residues has been built based on SeMet SAD data at 1.8 Å resolution. Unexpectedly, the structure revealed a novel metal centre in the amidohydrolase superfamily.
|Number of pages||2|
|Journal||Acta Crystallographica Section D: Biological Crystallography|
|Publication status||Published - Sep 2002|
ASJC Scopus subject areas
- Structural Biology