Crystal structure of a nucleoside diphosphate kinase from Bacillus halodenitrificans

Coexpression of its activity with a Mn-superoxide dismutase

Chun Jung Chen, Ming Yih Liu, Tschining Chang, Wen Chang Chang, Bi Cheng Wang, Jean Le Gall

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

We found that when grown under anaerobic conditions the moderate halophile, gram-positive bacterium Bacillus halodenitrificans (ATCC 49067) synthesizes large amounts of a polypeptide complex that contains a heme center capable of reversibly bind nitric oxide. This complex, when exposed to air, dissociates and reassociates into two active components, a Mn-containing superoxide dismutase (SOD) and a nucleoside diphosphate kinase (BhNDK). The crystal structure of this latter enzyme has been determined at 2.2Å resolution using molecular replacement method, based on the crystal structure of Drosophila melanogaster NDK. The model contains 149 residues of a total 150 residues and 34 water molecules. BhNDK consists of a four-stranded antiparallel β-sheet, whose surfaces are partially covered by six α-helices, and its overall and active site structures are similar to those of homologous enzymes. However, the hexameric packing of BhNDK shows that this enzyme is different from both eukaryotic and gram-negative bacteria. The need for the bacterium to presynthesize both SOD and NDK precursors which are activated during the anaerobic-aerobic transition is discussed.

Original languageEnglish
Pages (from-to)247-255
Number of pages9
JournalJournal of Structural Biology
Volume142
Issue number2
DOIs
Publication statusPublished - May 1 2003
Externally publishedYes

Fingerprint

Nucleoside-Diphosphate Kinase
Bacillus
Superoxide Dismutase
Enzymes
Gram-Positive Bacteria
Drosophila melanogaster
Gram-Negative Bacteria
Heme
Catalytic Domain
Nitric Oxide
Air
Bacteria
Peptides
Water

Keywords

  • Anaerobic
  • Denitrification
  • Halophile
  • Nucleoside diphosphate kinase

ASJC Scopus subject areas

  • Structural Biology

Cite this

Crystal structure of a nucleoside diphosphate kinase from Bacillus halodenitrificans : Coexpression of its activity with a Mn-superoxide dismutase. / Chen, Chun Jung; Liu, Ming Yih; Chang, Tschining; Chang, Wen Chang; Wang, Bi Cheng; Le Gall, Jean.

In: Journal of Structural Biology, Vol. 142, No. 2, 01.05.2003, p. 247-255.

Research output: Contribution to journalArticle

Chen, Chun Jung ; Liu, Ming Yih ; Chang, Tschining ; Chang, Wen Chang ; Wang, Bi Cheng ; Le Gall, Jean. / Crystal structure of a nucleoside diphosphate kinase from Bacillus halodenitrificans : Coexpression of its activity with a Mn-superoxide dismutase. In: Journal of Structural Biology. 2003 ; Vol. 142, No. 2. pp. 247-255.
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