Crystal Structure and Potential Head-to-Middle Condensation Function of a Z,Z-Farnesyl Diphosphate Synthase

Yueh Te Chan, Tzu Ping Ko, Shan Hsueh Yao, Ya Wen Chen, Cheng Chung Lee, Andrew H.J. Wang

Research output: Contribution to journalArticlepeer-review

19 Citations (Scopus)

Abstract

Plants produce a wide variety of secondary metabolites in response to adverse environmental factors. Z,Z-Farnesyl diphosphate (Z,Z-FPP), synthesized by Z,Z-farnesyl diphosphate synthase (zFPS), supports the formation of phytochemicals in wild tomatoes. Here, the crystal structure of N-terminal truncated zFPS (ΔzFPS) was determined. Irregular products including lavandulyl diphosphate and an unknown compound were surprisingly found. Apart from the truncated N-terminus as a functional regulator, structure-based analysis and mutagenesis assays revealed a residue H103 in ΔzFPS as one of the key elements to this irregular function. A series of substrate-enzyme complex structures were obtained from ΔzFPS-H103Y by co-crystallizing with isopentenyl diphosphate, dimethylallyl thiolodiphosphate, or both. Various substrate-binding modes were revealed. The catalytic mechanisms of both the head-to-tail and head-to-middle reactions in ΔzFPS were proposed. Functional switch between the two mechanisms in this enzyme and the essential role played by the flexible C-terminus were elucidated as well.

Original languageEnglish
Pages (from-to)930-936
Number of pages7
JournalACS Omega
Volume2
Issue number3
DOIs
Publication statusPublished - Mar 31 2017

ASJC Scopus subject areas

  • Chemistry(all)
  • Chemical Engineering(all)

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