Abstract
Objective: To examine the effect of vitrification with ethylene glycol (EG) for mature human oocytes in straws. Design: Prospective, randomized, in vitro experiments. Setting: Reproductive unit of a university hospital. Patient(s): Immature oocytes from 110 patients undergoing intracytoplasmic sperm injection (ICSI). Intervention(s): The immature oocytes were incubated to reach metaphase II (MII). The MII oocytes were treated with EG-based cryoprotectants and vitrified in straws. They were diluted in sucrose solutions, inseminated by ICSI, and cultured in vitro. Main Outcome Measure(s): Survival, fertilization, and embryo cleavage. Result(s): The survival rates were greater for oocytes pretreated with 1.5 M of EG (65% for 0 minute, 93% for 5 minutes, and 96% for 10 minutes). The oocytes vitrified in 60 and 90 seconds had a greater rate of fertilization than those vitrified in 120 seconds. There were no differences in survival and fertilization for vitrified oocytes diluted by three or four steps. The cleavage rates to the six- to eight-cell stage were comparable with controls. However, no blastocyst formation was observed in vitrified oocytes. Conclusion(s): Vitrification of human oocytes with EG in straws achieves a high rate of survival, fertilization, and early cleavage of embryos. Further studies should be conducted for the improvement of blastocyst formation. (C) 2000 by American Society for Reproductive Medicine.
| Original language | English |
|---|---|
| Pages (from-to) | 804-808 |
| Number of pages | 5 |
| Journal | Fertility and Sterility |
| Volume | 74 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - Oct 1 2000 |
| Externally published | Yes |
Fingerprint
Keywords
- Ethylene glycol
- Human oocytes
- Straw
- Vitrification
ASJC Scopus subject areas
- Reproductive Medicine
- Obstetrics and Gynaecology
Cite this
Cryopreservation of mature human oocytes by vitrification with ethylene glycol in straws. / Chen, Shee Uan; Lien, Yih Ron; Chao, Kuang Han; Lu, Hsin Fen; Ho, Hong Nerng; Yang, Yu Shih.
In: Fertility and Sterility, Vol. 74, No. 4, 01.10.2000, p. 804-808.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Cryopreservation of mature human oocytes by vitrification with ethylene glycol in straws
AU - Chen, Shee Uan
AU - Lien, Yih Ron
AU - Chao, Kuang Han
AU - Lu, Hsin Fen
AU - Ho, Hong Nerng
AU - Yang, Yu Shih
PY - 2000/10/1
Y1 - 2000/10/1
N2 - Objective: To examine the effect of vitrification with ethylene glycol (EG) for mature human oocytes in straws. Design: Prospective, randomized, in vitro experiments. Setting: Reproductive unit of a university hospital. Patient(s): Immature oocytes from 110 patients undergoing intracytoplasmic sperm injection (ICSI). Intervention(s): The immature oocytes were incubated to reach metaphase II (MII). The MII oocytes were treated with EG-based cryoprotectants and vitrified in straws. They were diluted in sucrose solutions, inseminated by ICSI, and cultured in vitro. Main Outcome Measure(s): Survival, fertilization, and embryo cleavage. Result(s): The survival rates were greater for oocytes pretreated with 1.5 M of EG (65% for 0 minute, 93% for 5 minutes, and 96% for 10 minutes). The oocytes vitrified in 60 and 90 seconds had a greater rate of fertilization than those vitrified in 120 seconds. There were no differences in survival and fertilization for vitrified oocytes diluted by three or four steps. The cleavage rates to the six- to eight-cell stage were comparable with controls. However, no blastocyst formation was observed in vitrified oocytes. Conclusion(s): Vitrification of human oocytes with EG in straws achieves a high rate of survival, fertilization, and early cleavage of embryos. Further studies should be conducted for the improvement of blastocyst formation. (C) 2000 by American Society for Reproductive Medicine.
AB - Objective: To examine the effect of vitrification with ethylene glycol (EG) for mature human oocytes in straws. Design: Prospective, randomized, in vitro experiments. Setting: Reproductive unit of a university hospital. Patient(s): Immature oocytes from 110 patients undergoing intracytoplasmic sperm injection (ICSI). Intervention(s): The immature oocytes were incubated to reach metaphase II (MII). The MII oocytes were treated with EG-based cryoprotectants and vitrified in straws. They were diluted in sucrose solutions, inseminated by ICSI, and cultured in vitro. Main Outcome Measure(s): Survival, fertilization, and embryo cleavage. Result(s): The survival rates were greater for oocytes pretreated with 1.5 M of EG (65% for 0 minute, 93% for 5 minutes, and 96% for 10 minutes). The oocytes vitrified in 60 and 90 seconds had a greater rate of fertilization than those vitrified in 120 seconds. There were no differences in survival and fertilization for vitrified oocytes diluted by three or four steps. The cleavage rates to the six- to eight-cell stage were comparable with controls. However, no blastocyst formation was observed in vitrified oocytes. Conclusion(s): Vitrification of human oocytes with EG in straws achieves a high rate of survival, fertilization, and early cleavage of embryos. Further studies should be conducted for the improvement of blastocyst formation. (C) 2000 by American Society for Reproductive Medicine.
KW - Ethylene glycol
KW - Human oocytes
KW - Straw
KW - Vitrification
UR - http://www.scopus.com/inward/record.url?scp=0033795373&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0033795373&partnerID=8YFLogxK
U2 - 10.1016/S0015-0282(00)01516-8
DO - 10.1016/S0015-0282(00)01516-8
M3 - Article
C2 - 11020527
AN - SCOPUS:0033795373
VL - 74
SP - 804
EP - 808
JO - Fertility and Sterility
JF - Fertility and Sterility
SN - 0015-0282
IS - 4
ER -