Cryogen spray cooling mitigates inflammation and injury-induced CISD2 decline in rat spinal cord hemisection model

Woon Man Kung, Cheng Jen Chang, Tzu Yung Chen, Muh Shi Lin

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

Therapeutic strategies for traumatic spinal cord injury generally involve rectifying concomitant destruction to the spinal cord from inflammation, mitochondrial dysfunction, and eventual neuronal apoptosis. Elevating the expression of spinal cord injury-attenuated CDGSH iron-sulfur domain-2 has been shown to mitigate the pathologies above. In the current work, hypothermia was induced via continuous cryogen spray cooling in a rat spinal cord hemisection model. Spinal cord injury was shown to elevate the mRNA expression of proinflammatory mediators, including NFκB, iNOS, TNF-α, and regulated upon activation, normal T-cell expressed and secreted as well as lower CDGSH iron-sulfur domain-2 expression. Cryogen spray cooling treatment was shown to attenuate inflammatory reactions and elevate CDGSH iron-sulfur domain- 2 expression. Immunohistochemical analysis of the glial fibrillary acidic protein, caspase-3 and NeuN in spinal cord injured rats that underwent cryogen spray cooling treatment revealed notable reductions in injury-induced astrocytic activation, apoptosis, neuronal loss, and decline in CDGSH iron-sulfur domain-2 expression. These results demonstrate the CDGSH iron-sulfur domain-2 preserving effects of cryogen spray cooling, which could contribute to the prevention of astrocytic activation, astrocyte-mediated neuroinflammation, apoptosis, and neuron loss.

Original languageEnglish
Pages (from-to)619-628
Number of pages10
JournalJournal of Integrative Neuroscience
Volume19
Issue number4
DOIs
Publication statusPublished - Dec 30 2020

Keywords

  • Apoptosis
  • Astrocyte activation
  • CISD2
  • Cryogen spray cooling
  • Hypothermia
  • Inflammatory response
  • Neuronal loss
  • Spinal cord injury

ASJC Scopus subject areas

  • Neuroscience(all)

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