Crosstalk between integrin αvβ3 and estrogen receptor-α is involved in thyroid hormone-induced proliferation in human lung carcinoma cells

Ran Meng, Heng Yuan Tang, Jennifer Westfall, David London, James H. Cao, Shaker A. Mousa, Mary Luidens, Aleck Hercbergs, Faith B. Davis, Paul J. Davis, Hung Yun Lin

Research output: Contribution to journalArticle

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Abstract

A cell surface receptor for thyroid hormone that activates extracellular regulated kinase (ERK) 1/2 has been identified on integrin αvβ3. We have examined the actions of thyroid hormone initiated at the integrin on human NCI-H522 non-small cell lung carcinoma and NCI-H510A small cell lung cancer cells. At a physiologic total hormone concentration (10 -7 M), T 4 significantly increased proliferating cell nuclear antigen (PCNA) abundance in these cell lines, as did 3, 5, 3′-triiodo-L-thyronine (T 3) at a supraphysiologic concentration. Neutralizing antibody to integrin αvβ3 and an integrin-binding Arg-Gly-Asp (RGD) peptide blocked thyroid hormone-induced PCNA expression. Tetraiodothyroacetic acid (tetrac) lacks thyroid hormone function but inhibits binding of T 4 and T 3 to the integrin receptor; tetrac eliminated thyroid hormone-induced lung cancer cell proliferation and ERK1/2 activation. In these estrogen receptor-α (ERα)-positive lung cancer cells, thyroid hormone (T 4&T 3) caused phosphorylation of ERα; the specific ERα antagonist ICI 182,780 blocked T 4-induced, but not T 3-induced ERK1/2 activation, as well as ERα phosphorylation, proliferating-cell nuclear antigen (PCNA) expression and hormone-dependent thymidine uptake by tumor cells. Thus, in ERα-positive human lung cancer cells, the proliferative action of thyroid hormone initiated at the plasma membrane is at least in part mediated by ERα. In summary, thyroid hormone may be one of several endogenous factors capable of supporting proliferation of lung cancer cells. Activity as an inhibitor of lung cancer cell proliferation induced at the integrin receptor makes tetrac a novel anti-proliferative agent.

Original languageEnglish
Article numbere27547
JournalPLoS One
Volume6
Issue number11
DOIs
Publication statusPublished - Nov 22 2011
Externally publishedYes

Fingerprint

integrins
thyroid hormones
Crosstalk
Thyroid Hormones
Integrins
Estrogen Receptors
carcinoma
lung neoplasms
lungs
Cells
Carcinoma
Lung
proliferating cell nuclear antigen
Lung Neoplasms
Proliferating Cell Nuclear Antigen
cells
Phosphorylation
Cell proliferation
acids
cell proliferation

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Crosstalk between integrin αvβ3 and estrogen receptor-α is involved in thyroid hormone-induced proliferation in human lung carcinoma cells. / Meng, Ran; Tang, Heng Yuan; Westfall, Jennifer; London, David; Cao, James H.; Mousa, Shaker A.; Luidens, Mary; Hercbergs, Aleck; Davis, Faith B.; Davis, Paul J.; Lin, Hung Yun.

In: PLoS One, Vol. 6, No. 11, e27547, 22.11.2011.

Research output: Contribution to journalArticle

Meng, R, Tang, HY, Westfall, J, London, D, Cao, JH, Mousa, SA, Luidens, M, Hercbergs, A, Davis, FB, Davis, PJ & Lin, HY 2011, 'Crosstalk between integrin αvβ3 and estrogen receptor-α is involved in thyroid hormone-induced proliferation in human lung carcinoma cells', PLoS One, vol. 6, no. 11, e27547. https://doi.org/10.1371/journal.pone.0027547
Meng, Ran ; Tang, Heng Yuan ; Westfall, Jennifer ; London, David ; Cao, James H. ; Mousa, Shaker A. ; Luidens, Mary ; Hercbergs, Aleck ; Davis, Faith B. ; Davis, Paul J. ; Lin, Hung Yun. / Crosstalk between integrin αvβ3 and estrogen receptor-α is involved in thyroid hormone-induced proliferation in human lung carcinoma cells. In: PLoS One. 2011 ; Vol. 6, No. 11.
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AU - Mousa, Shaker A.

AU - Luidens, Mary

AU - Hercbergs, Aleck

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