Coupling dichloroacetate treatment with curcumin significantly enhances anticancer potential

Ping Chuan Kan, Yu Jia Chang, Chin Sung Chien, Chen Ying Su, Hsu Wei Fang

Research output: Contribution to journalArticle

Abstract

Background/Aim: Dichloroacetate (DCA) and curcumin have been shown to be potent drug candidates in cancer therapy. Our study aimed to investigate the combined effects of DCA and essential oil-blended curcumin (ECUR) using the hepatoma Huh-7 cell model. Materials and Methods: Muse Cell Cycle assay, Muse Annexin V & Dead Cell assay, Muse Oxidative Stress assay, and western blot analysis were applied to explore the underlying mechanisms. Results: DCA combined with ECUR dramatically augmented inhibition of cell survival and enhanced apoptotic induction. The enhanced apoptosis was accompanied by mitochondria-dependent apoptotic signaling activation and corroborated with significant cellular morphological alternations. Conclusion: Apoptosis was the major event contributing to the synergistically boosted antiproliferative effect. Coupling DCA treatment with curcumin may rationally be expected to lower the DCA dose needed and relatively reduce accompanying toxicity and oxidative damage while enhancing anticancer potential. This novel 'add-on' approach is, thus, of enormous value to the current DCA therapy.

Original languageEnglish
Pages (from-to)6253-6261
Number of pages9
JournalAnticancer Research
Volume38
Issue number11
DOIs
Publication statusPublished - Nov 1 2018

Fingerprint

Curcumin
Alprostadil
Apoptosis
Annexin A5
Volatile Oils
Hepatocellular Carcinoma
Cell Survival
Cell Cycle
Mitochondria
Oxidative Stress
Western Blotting
Therapeutics
Pharmaceutical Preparations
Neoplasms

Keywords

  • Combination therapy
  • Curcumin
  • Dichloroacetate

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Coupling dichloroacetate treatment with curcumin significantly enhances anticancer potential. / Kan, Ping Chuan; Chang, Yu Jia; Chien, Chin Sung; Su, Chen Ying; Fang, Hsu Wei.

In: Anticancer Research, Vol. 38, No. 11, 01.11.2018, p. 6253-6261.

Research output: Contribution to journalArticle

Kan, Ping Chuan ; Chang, Yu Jia ; Chien, Chin Sung ; Su, Chen Ying ; Fang, Hsu Wei. / Coupling dichloroacetate treatment with curcumin significantly enhances anticancer potential. In: Anticancer Research. 2018 ; Vol. 38, No. 11. pp. 6253-6261.
@article{aa70c5bd9c354fe19249c91a8e4c66fa,
title = "Coupling dichloroacetate treatment with curcumin significantly enhances anticancer potential",
abstract = "Background/Aim: Dichloroacetate (DCA) and curcumin have been shown to be potent drug candidates in cancer therapy. Our study aimed to investigate the combined effects of DCA and essential oil-blended curcumin (ECUR) using the hepatoma Huh-7 cell model. Materials and Methods: Muse™ Cell Cycle assay, Muse™ Annexin V & Dead Cell assay, Muse™ Oxidative Stress assay, and western blot analysis were applied to explore the underlying mechanisms. Results: DCA combined with ECUR dramatically augmented inhibition of cell survival and enhanced apoptotic induction. The enhanced apoptosis was accompanied by mitochondria-dependent apoptotic signaling activation and corroborated with significant cellular morphological alternations. Conclusion: Apoptosis was the major event contributing to the synergistically boosted antiproliferative effect. Coupling DCA treatment with curcumin may rationally be expected to lower the DCA dose needed and relatively reduce accompanying toxicity and oxidative damage while enhancing anticancer potential. This novel 'add-on' approach is, thus, of enormous value to the current DCA therapy.",
keywords = "Combination therapy, Curcumin, Dichloroacetate",
author = "Kan, {Ping Chuan} and Chang, {Yu Jia} and Chien, {Chin Sung} and Su, {Chen Ying} and Fang, {Hsu Wei}",
year = "2018",
month = "11",
day = "1",
doi = "10.21873/anticanres.12981",
language = "English",
volume = "38",
pages = "6253--6261",
journal = "Anticancer Research",
issn = "0250-7005",
publisher = "International Institute of Anticancer Research",
number = "11",

}

TY - JOUR

T1 - Coupling dichloroacetate treatment with curcumin significantly enhances anticancer potential

AU - Kan, Ping Chuan

AU - Chang, Yu Jia

AU - Chien, Chin Sung

AU - Su, Chen Ying

AU - Fang, Hsu Wei

PY - 2018/11/1

Y1 - 2018/11/1

N2 - Background/Aim: Dichloroacetate (DCA) and curcumin have been shown to be potent drug candidates in cancer therapy. Our study aimed to investigate the combined effects of DCA and essential oil-blended curcumin (ECUR) using the hepatoma Huh-7 cell model. Materials and Methods: Muse™ Cell Cycle assay, Muse™ Annexin V & Dead Cell assay, Muse™ Oxidative Stress assay, and western blot analysis were applied to explore the underlying mechanisms. Results: DCA combined with ECUR dramatically augmented inhibition of cell survival and enhanced apoptotic induction. The enhanced apoptosis was accompanied by mitochondria-dependent apoptotic signaling activation and corroborated with significant cellular morphological alternations. Conclusion: Apoptosis was the major event contributing to the synergistically boosted antiproliferative effect. Coupling DCA treatment with curcumin may rationally be expected to lower the DCA dose needed and relatively reduce accompanying toxicity and oxidative damage while enhancing anticancer potential. This novel 'add-on' approach is, thus, of enormous value to the current DCA therapy.

AB - Background/Aim: Dichloroacetate (DCA) and curcumin have been shown to be potent drug candidates in cancer therapy. Our study aimed to investigate the combined effects of DCA and essential oil-blended curcumin (ECUR) using the hepatoma Huh-7 cell model. Materials and Methods: Muse™ Cell Cycle assay, Muse™ Annexin V & Dead Cell assay, Muse™ Oxidative Stress assay, and western blot analysis were applied to explore the underlying mechanisms. Results: DCA combined with ECUR dramatically augmented inhibition of cell survival and enhanced apoptotic induction. The enhanced apoptosis was accompanied by mitochondria-dependent apoptotic signaling activation and corroborated with significant cellular morphological alternations. Conclusion: Apoptosis was the major event contributing to the synergistically boosted antiproliferative effect. Coupling DCA treatment with curcumin may rationally be expected to lower the DCA dose needed and relatively reduce accompanying toxicity and oxidative damage while enhancing anticancer potential. This novel 'add-on' approach is, thus, of enormous value to the current DCA therapy.

KW - Combination therapy

KW - Curcumin

KW - Dichloroacetate

UR - http://www.scopus.com/inward/record.url?scp=85056130515&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85056130515&partnerID=8YFLogxK

U2 - 10.21873/anticanres.12981

DO - 10.21873/anticanres.12981

M3 - Article

C2 - 30396945

AN - SCOPUS:85056130515

VL - 38

SP - 6253

EP - 6261

JO - Anticancer Research

JF - Anticancer Research

SN - 0250-7005

IS - 11

ER -