Corticotropin-releasing factor-induced production of cyclic AMP by human peripheral blood immunocytes

Vijendra K. Singh, Sy Jye Christine Leu

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

The immunomodulating properties of a neuropeptide hormone, corticotropin-releasing factor (CRF), led us to investigate its effect on cAMP production by human peripheral blood mononuclear cells (MNC). In response to stimulation with CRF (100 nM), a statistically significant (P = 0.019) increase occurred in the amount of cAMP produced by MNC. Purified monocytes, but not lymphocytes, also displayed a significant (P = 0.01) increase (8- to 10-fold) in intracellular cAMP after treatment with CRF (100 nM). The antagonist α-helical CRF9-41 (100 nM) counteracted the cAMP increase induced by CRF (100 nM). The CRF-induced cAMP production was augmented by pretreatment of MNC with a cAMP-dependent protein kinase (PKA) peptide inhibitor (PI20), but was virtually unaffected by the protein kinase C (PKC) inhibitor H7, suggesting a role for cAMP signalling. Moreover, the CRF-stimulated cAMP level was reduced to baseline by intracellular Ca2+ antagonist HA1004, indicating a role for Ca2+-signalling. Based on these findings, it is concluded that cAMP and/or Ca2+ play a second messenger role in the CRF signal transduction pathway.

Original languageEnglish
Pages (from-to)239-245
Number of pages7
JournalImmunology Letters
Volume35
Issue number3
DOIs
Publication statusPublished - 1993
Externally publishedYes

Keywords

  • cAMP
  • Corticotropin-releasing factor
  • Immunomodulation
  • Neuroendocrine-immune axis
  • Neurohormone
  • Neuropeptide

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

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