Correlations between cytoplasmic CSE1L in neoplastic colorectal glands and depth of tumor penetration and cancer stage

Cheng Jeng Tai, Tzu Cheng Su, Ming Chung Jiang, Hung Chang Chen, Shing Chuan Shen, Woan Ruoh Lee, Ching Fong Liao, Ying Chun Chen, Shu Hui Lin, Li Tzu Li, Ko Hung Shen, Chung Min Yeh, Kun Tu Yeh, Ching Hsiao Lee, Hsin Yi Shih, Chun Chao Chang

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Background: Colorectal carcinomas spread easily to nearby tissues around the colon or rectum, and display strong potential for invasion and metastasis. CSE1L, the chromosome segregation 1-like protein, is implicated in cancer progression and is located in both the cytoplasm and nuclei of tumor cells. We investigated the prognostic significance of cytoplasmic vs. nuclear CSE1L expression in colorectal cancer.Methods: The invasion- and metastasis-stimulating activities of CSE1L were studied by in vitro invasion and animal experiments. CSE1L expression in colorectal cancer was assayed by immunohistochemistry, with tissue microarray consisting of 128 surgically resected specimens; and scored using a semiquantitative method. The correlations between CSE1L expression and clinicopathological parameters were analyzed.Results: CSE1L overexpression was associated with increased invasiveness and metastasis of cancer cells. Non-neoplastic colorectal glands showed minimal CSE1L staining, whereas most colorectal carcinomas (99.2%, 127/128) were significantly positive for CSE1L staining. Cytoplasmic CSE1L was associated with cancer stage (P=0.003) and depth of tumor penetration (P=0.007). Cytoplasmic CSE1L expression also correlated with lymph node metastasis of the disease in Cox regression analysis. Conclusions: CSE1L regulates the invasiveness and metastasis of cancer cells, and immunohistochemical analysis of cytoplasmic CSE1L in colorectal tumors may provide a useful aid to prognosis.

Original languageEnglish
Article number29
JournalJournal of Translational Medicine
Volume11
Issue number1
DOIs
Publication statusPublished - Jan 31 2013

Fingerprint

Tumors
Cells
Colorectal Neoplasms
Cellular Apoptosis Susceptibility Protein
Neoplasm Metastasis
Tissue
Neoplasms
Microarrays
Regression analysis
Animals
Staining and Labeling
Cell Nucleus
Rectum
Colon
Cytoplasm
Lymph Nodes
Experiments
Immunohistochemistry
Regression Analysis

Keywords

  • Colorectal cancer
  • CSE1L
  • Cytoplasm
  • Invasion
  • Metastasis
  • Nucleus

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Correlations between cytoplasmic CSE1L in neoplastic colorectal glands and depth of tumor penetration and cancer stage. / Tai, Cheng Jeng; Su, Tzu Cheng; Jiang, Ming Chung; Chen, Hung Chang; Shen, Shing Chuan; Lee, Woan Ruoh; Liao, Ching Fong; Chen, Ying Chun; Lin, Shu Hui; Li, Li Tzu; Shen, Ko Hung; Yeh, Chung Min; Yeh, Kun Tu; Lee, Ching Hsiao; Shih, Hsin Yi; Chang, Chun Chao.

In: Journal of Translational Medicine, Vol. 11, No. 1, 29, 31.01.2013.

Research output: Contribution to journalArticle

Tai, Cheng Jeng ; Su, Tzu Cheng ; Jiang, Ming Chung ; Chen, Hung Chang ; Shen, Shing Chuan ; Lee, Woan Ruoh ; Liao, Ching Fong ; Chen, Ying Chun ; Lin, Shu Hui ; Li, Li Tzu ; Shen, Ko Hung ; Yeh, Chung Min ; Yeh, Kun Tu ; Lee, Ching Hsiao ; Shih, Hsin Yi ; Chang, Chun Chao. / Correlations between cytoplasmic CSE1L in neoplastic colorectal glands and depth of tumor penetration and cancer stage. In: Journal of Translational Medicine. 2013 ; Vol. 11, No. 1.
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abstract = "Background: Colorectal carcinomas spread easily to nearby tissues around the colon or rectum, and display strong potential for invasion and metastasis. CSE1L, the chromosome segregation 1-like protein, is implicated in cancer progression and is located in both the cytoplasm and nuclei of tumor cells. We investigated the prognostic significance of cytoplasmic vs. nuclear CSE1L expression in colorectal cancer.Methods: The invasion- and metastasis-stimulating activities of CSE1L were studied by in vitro invasion and animal experiments. CSE1L expression in colorectal cancer was assayed by immunohistochemistry, with tissue microarray consisting of 128 surgically resected specimens; and scored using a semiquantitative method. The correlations between CSE1L expression and clinicopathological parameters were analyzed.Results: CSE1L overexpression was associated with increased invasiveness and metastasis of cancer cells. Non-neoplastic colorectal glands showed minimal CSE1L staining, whereas most colorectal carcinomas (99.2{\%}, 127/128) were significantly positive for CSE1L staining. Cytoplasmic CSE1L was associated with cancer stage (P=0.003) and depth of tumor penetration (P=0.007). Cytoplasmic CSE1L expression also correlated with lymph node metastasis of the disease in Cox regression analysis. Conclusions: CSE1L regulates the invasiveness and metastasis of cancer cells, and immunohistochemical analysis of cytoplasmic CSE1L in colorectal tumors may provide a useful aid to prognosis.",
keywords = "Colorectal cancer, CSE1L, Cytoplasm, Invasion, Metastasis, Nucleus",
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T1 - Correlations between cytoplasmic CSE1L in neoplastic colorectal glands and depth of tumor penetration and cancer stage

AU - Tai, Cheng Jeng

AU - Su, Tzu Cheng

AU - Jiang, Ming Chung

AU - Chen, Hung Chang

AU - Shen, Shing Chuan

AU - Lee, Woan Ruoh

AU - Liao, Ching Fong

AU - Chen, Ying Chun

AU - Lin, Shu Hui

AU - Li, Li Tzu

AU - Shen, Ko Hung

AU - Yeh, Chung Min

AU - Yeh, Kun Tu

AU - Lee, Ching Hsiao

AU - Shih, Hsin Yi

AU - Chang, Chun Chao

PY - 2013/1/31

Y1 - 2013/1/31

N2 - Background: Colorectal carcinomas spread easily to nearby tissues around the colon or rectum, and display strong potential for invasion and metastasis. CSE1L, the chromosome segregation 1-like protein, is implicated in cancer progression and is located in both the cytoplasm and nuclei of tumor cells. We investigated the prognostic significance of cytoplasmic vs. nuclear CSE1L expression in colorectal cancer.Methods: The invasion- and metastasis-stimulating activities of CSE1L were studied by in vitro invasion and animal experiments. CSE1L expression in colorectal cancer was assayed by immunohistochemistry, with tissue microarray consisting of 128 surgically resected specimens; and scored using a semiquantitative method. The correlations between CSE1L expression and clinicopathological parameters were analyzed.Results: CSE1L overexpression was associated with increased invasiveness and metastasis of cancer cells. Non-neoplastic colorectal glands showed minimal CSE1L staining, whereas most colorectal carcinomas (99.2%, 127/128) were significantly positive for CSE1L staining. Cytoplasmic CSE1L was associated with cancer stage (P=0.003) and depth of tumor penetration (P=0.007). Cytoplasmic CSE1L expression also correlated with lymph node metastasis of the disease in Cox regression analysis. Conclusions: CSE1L regulates the invasiveness and metastasis of cancer cells, and immunohistochemical analysis of cytoplasmic CSE1L in colorectal tumors may provide a useful aid to prognosis.

AB - Background: Colorectal carcinomas spread easily to nearby tissues around the colon or rectum, and display strong potential for invasion and metastasis. CSE1L, the chromosome segregation 1-like protein, is implicated in cancer progression and is located in both the cytoplasm and nuclei of tumor cells. We investigated the prognostic significance of cytoplasmic vs. nuclear CSE1L expression in colorectal cancer.Methods: The invasion- and metastasis-stimulating activities of CSE1L were studied by in vitro invasion and animal experiments. CSE1L expression in colorectal cancer was assayed by immunohistochemistry, with tissue microarray consisting of 128 surgically resected specimens; and scored using a semiquantitative method. The correlations between CSE1L expression and clinicopathological parameters were analyzed.Results: CSE1L overexpression was associated with increased invasiveness and metastasis of cancer cells. Non-neoplastic colorectal glands showed minimal CSE1L staining, whereas most colorectal carcinomas (99.2%, 127/128) were significantly positive for CSE1L staining. Cytoplasmic CSE1L was associated with cancer stage (P=0.003) and depth of tumor penetration (P=0.007). Cytoplasmic CSE1L expression also correlated with lymph node metastasis of the disease in Cox regression analysis. Conclusions: CSE1L regulates the invasiveness and metastasis of cancer cells, and immunohistochemical analysis of cytoplasmic CSE1L in colorectal tumors may provide a useful aid to prognosis.

KW - Colorectal cancer

KW - CSE1L

KW - Cytoplasm

KW - Invasion

KW - Metastasis

KW - Nucleus

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