Correlation of HAMP gene polymorphisms and expression with the susceptibility and length of hospital stays in Taiwanese children with Kawasaki disease

Ying Hsien Huang, Kuender D. Yang, Yu Wen Hsu, Hsing Fang Lu, Henry Sung Ching Wong, Hong Ren Yu, Hsing Chun Kuo, Fu Chen Huang, Mao Hung Lo, Kai Sheng Hsieh, Su Fen Chen, Wei Chiao Chang, Ho Chang Kuo

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Kawasaki disease (KD) is a form of systemic vasculitis. Regarding its pathogenesis, HAMP gene encoding hepcidin, which is significant for iron metabolism, has a vital function. In this study, we recruited a total of 381 KD patients for genotyping. Data from 997 subjects (500 subjects from cohort 1; 497 subjects from cohort 2) were used for analysis. Using TaqMan allelic discrimination, we determined five tag SNPs (rs916145, rs10421768, rs3817623, rs7251432, and rs2293689). Treatment outcome data related to such clinical phenotypes as coronary artery lesions (CAL), coronary artery aneurysms (CAA), and intravenous immunoglobulin (IVIG) effects were also collected. Furthermore, we measured plasma hepcidin levels with an enzyme-linked immunosorbent assay. We found that HAMP gene polymorphism (rs7251432, and rs2293689) was significantly correlated with KD risk and that plasma hepcidin levels both before and after IVIG treatment had a significantly positive correlation with length of hospital stays (R = 0.217, p = 0.046 and R = 0.381, p < 0.0001, respectively). In contrast, plasma hepcidin levels has a negative correlation with KD patients' albumin levels (R = -0.27, p < 0.001) prior to IVIG treatment. This study's findings indicate that HAMP might have a role in the disease susceptibility, as well as its expressions correlated length of hospital stays, and albumin levels in Taiwanese children with KD.

Original languageEnglish
Pages (from-to)51859-51868
Number of pages10
JournalOncotarget
Volume8
Issue number31
DOIs
Publication statusPublished - Feb 7 2017

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Mucocutaneous Lymph Node Syndrome
Hepcidins
Length of Stay
Intravenous Immunoglobulins
Gene Expression
Albumins
Coronary Vessels
Coronary Aneurysm
Systemic Vasculitis
Disease Susceptibility
Genes
Single Nucleotide Polymorphism
Iron
Enzyme-Linked Immunosorbent Assay
Phenotype
Therapeutics

Keywords

  • Genetic polymorphism
  • Hepcidin
  • Kawasaki disease

ASJC Scopus subject areas

  • Oncology

Cite this

Correlation of HAMP gene polymorphisms and expression with the susceptibility and length of hospital stays in Taiwanese children with Kawasaki disease. / Huang, Ying Hsien; Yang, Kuender D.; Hsu, Yu Wen; Lu, Hsing Fang; Wong, Henry Sung Ching; Yu, Hong Ren; Kuo, Hsing Chun; Huang, Fu Chen; Lo, Mao Hung; Hsieh, Kai Sheng; Chen, Su Fen; Chang, Wei Chiao; Kuo, Ho Chang.

In: Oncotarget, Vol. 8, No. 31, 07.02.2017, p. 51859-51868.

Research output: Contribution to journalArticle

Huang, YH, Yang, KD, Hsu, YW, Lu, HF, Wong, HSC, Yu, HR, Kuo, HC, Huang, FC, Lo, MH, Hsieh, KS, Chen, SF, Chang, WC & Kuo, HC 2017, 'Correlation of HAMP gene polymorphisms and expression with the susceptibility and length of hospital stays in Taiwanese children with Kawasaki disease', Oncotarget, vol. 8, no. 31, pp. 51859-51868. https://doi.org/10.18632/oncotarget.17700
Huang, Ying Hsien ; Yang, Kuender D. ; Hsu, Yu Wen ; Lu, Hsing Fang ; Wong, Henry Sung Ching ; Yu, Hong Ren ; Kuo, Hsing Chun ; Huang, Fu Chen ; Lo, Mao Hung ; Hsieh, Kai Sheng ; Chen, Su Fen ; Chang, Wei Chiao ; Kuo, Ho Chang. / Correlation of HAMP gene polymorphisms and expression with the susceptibility and length of hospital stays in Taiwanese children with Kawasaki disease. In: Oncotarget. 2017 ; Vol. 8, No. 31. pp. 51859-51868.
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AU - Lu, Hsing Fang

AU - Wong, Henry Sung Ching

AU - Yu, Hong Ren

AU - Kuo, Hsing Chun

AU - Huang, Fu Chen

AU - Lo, Mao Hung

AU - Hsieh, Kai Sheng

AU - Chen, Su Fen

AU - Chang, Wei Chiao

AU - Kuo, Ho Chang

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N2 - Kawasaki disease (KD) is a form of systemic vasculitis. Regarding its pathogenesis, HAMP gene encoding hepcidin, which is significant for iron metabolism, has a vital function. In this study, we recruited a total of 381 KD patients for genotyping. Data from 997 subjects (500 subjects from cohort 1; 497 subjects from cohort 2) were used for analysis. Using TaqMan allelic discrimination, we determined five tag SNPs (rs916145, rs10421768, rs3817623, rs7251432, and rs2293689). Treatment outcome data related to such clinical phenotypes as coronary artery lesions (CAL), coronary artery aneurysms (CAA), and intravenous immunoglobulin (IVIG) effects were also collected. Furthermore, we measured plasma hepcidin levels with an enzyme-linked immunosorbent assay. We found that HAMP gene polymorphism (rs7251432, and rs2293689) was significantly correlated with KD risk and that plasma hepcidin levels both before and after IVIG treatment had a significantly positive correlation with length of hospital stays (R = 0.217, p = 0.046 and R = 0.381, p < 0.0001, respectively). In contrast, plasma hepcidin levels has a negative correlation with KD patients' albumin levels (R = -0.27, p < 0.001) prior to IVIG treatment. This study's findings indicate that HAMP might have a role in the disease susceptibility, as well as its expressions correlated length of hospital stays, and albumin levels in Taiwanese children with KD.

AB - Kawasaki disease (KD) is a form of systemic vasculitis. Regarding its pathogenesis, HAMP gene encoding hepcidin, which is significant for iron metabolism, has a vital function. In this study, we recruited a total of 381 KD patients for genotyping. Data from 997 subjects (500 subjects from cohort 1; 497 subjects from cohort 2) were used for analysis. Using TaqMan allelic discrimination, we determined five tag SNPs (rs916145, rs10421768, rs3817623, rs7251432, and rs2293689). Treatment outcome data related to such clinical phenotypes as coronary artery lesions (CAL), coronary artery aneurysms (CAA), and intravenous immunoglobulin (IVIG) effects were also collected. Furthermore, we measured plasma hepcidin levels with an enzyme-linked immunosorbent assay. We found that HAMP gene polymorphism (rs7251432, and rs2293689) was significantly correlated with KD risk and that plasma hepcidin levels both before and after IVIG treatment had a significantly positive correlation with length of hospital stays (R = 0.217, p = 0.046 and R = 0.381, p < 0.0001, respectively). In contrast, plasma hepcidin levels has a negative correlation with KD patients' albumin levels (R = -0.27, p < 0.001) prior to IVIG treatment. This study's findings indicate that HAMP might have a role in the disease susceptibility, as well as its expressions correlated length of hospital stays, and albumin levels in Taiwanese children with KD.

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