Correlation between the MR T2 value at 4.7 T and relative water content in articular cartilage in experimental osteoarthritis induced by ACL transection

M. C. Chou, P. H. Tsai, G. S. Huang, H. S. Lee, C. H. Lee, M. H. Lin, C. Y. Lin, H. W. Chung

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

Objective: Both animal and human studies using magnetic resonance imaging (MRI) show that cartilage degeneration increases the T2 value. However, it is unclear whether the T2 value correlates linearly with water content in cartilage with osteoarthritis. The purpose of this study was to investigate the relationship between the T2 value and water content using an animal model of cartilage injury measured at 4.7 T. Design: Thirty Sprague Dawley rats were randomly separated into three groups (n = 10 for each group). Group 1 rats were not operated on (control). Group 2 rats received a sham operation, and group 3 rats received an anterior cruciate ligament (ACL) transection. Six rats of each group were randomly assigned to T2 measurement and later subjected to ex vivo analysis of the relative water content of the knee cartilage. The other four rats in each group were killed, and the severity of cartilage degeneration was examined histologically. The knees of the six rats in the ACL transection group were imaged sequentially 4 and 13 weeks after ACL transection, and the relative water content was measured at 13 weeks. Results: The cartilage T2 value was significantly higher 4 and 13 weeks after ACL transection in the operated knees than in the knees of the control and sham groups. The cartilage T2 value was significantly higher at 13 weeks than at 4 weeks in the operated knees. The T2 value was strongly positively correlated with the relative water content (R = 0.885, P <0.0001). Conclusion: The trend of changes in the T2 values is consistent with an increase in the relative water content in our cartilage degeneration model. This model has potential use for the clinical evaluation of osteoarthritis.

Original languageEnglish
Pages (from-to)441-447
Number of pages7
JournalOsteoarthritis and Cartilage
Volume17
Issue number4
DOIs
Publication statusPublished - Apr 2009
Externally publishedYes

Fingerprint

Anterior Cruciate Ligament
Ligaments
Cartilage
Articular Cartilage
Osteoarthritis
Water content
Rats
Water
Knee
Animals
Magnetic resonance
Sprague Dawley Rats
Animal Models
Magnetic Resonance Imaging
Imaging techniques
Control Groups
Wounds and Injuries

Keywords

  • ACL transection
  • Cartilage degeneration
  • Cartilage T2
  • Water content

ASJC Scopus subject areas

  • Biomedical Engineering
  • Orthopedics and Sports Medicine
  • Rheumatology
  • Medicine(all)

Cite this

Correlation between the MR T2 value at 4.7 T and relative water content in articular cartilage in experimental osteoarthritis induced by ACL transection. / Chou, M. C.; Tsai, P. H.; Huang, G. S.; Lee, H. S.; Lee, C. H.; Lin, M. H.; Lin, C. Y.; Chung, H. W.

In: Osteoarthritis and Cartilage, Vol. 17, No. 4, 04.2009, p. 441-447.

Research output: Contribution to journalArticle

Chou, M. C. ; Tsai, P. H. ; Huang, G. S. ; Lee, H. S. ; Lee, C. H. ; Lin, M. H. ; Lin, C. Y. ; Chung, H. W. / Correlation between the MR T2 value at 4.7 T and relative water content in articular cartilage in experimental osteoarthritis induced by ACL transection. In: Osteoarthritis and Cartilage. 2009 ; Vol. 17, No. 4. pp. 441-447.
@article{1bc6940942704879be60f39479396f6e,
title = "Correlation between the MR T2 value at 4.7 T and relative water content in articular cartilage in experimental osteoarthritis induced by ACL transection",
abstract = "Objective: Both animal and human studies using magnetic resonance imaging (MRI) show that cartilage degeneration increases the T2 value. However, it is unclear whether the T2 value correlates linearly with water content in cartilage with osteoarthritis. The purpose of this study was to investigate the relationship between the T2 value and water content using an animal model of cartilage injury measured at 4.7 T. Design: Thirty Sprague Dawley rats were randomly separated into three groups (n = 10 for each group). Group 1 rats were not operated on (control). Group 2 rats received a sham operation, and group 3 rats received an anterior cruciate ligament (ACL) transection. Six rats of each group were randomly assigned to T2 measurement and later subjected to ex vivo analysis of the relative water content of the knee cartilage. The other four rats in each group were killed, and the severity of cartilage degeneration was examined histologically. The knees of the six rats in the ACL transection group were imaged sequentially 4 and 13 weeks after ACL transection, and the relative water content was measured at 13 weeks. Results: The cartilage T2 value was significantly higher 4 and 13 weeks after ACL transection in the operated knees than in the knees of the control and sham groups. The cartilage T2 value was significantly higher at 13 weeks than at 4 weeks in the operated knees. The T2 value was strongly positively correlated with the relative water content (R = 0.885, P <0.0001). Conclusion: The trend of changes in the T2 values is consistent with an increase in the relative water content in our cartilage degeneration model. This model has potential use for the clinical evaluation of osteoarthritis.",
keywords = "ACL transection, Cartilage degeneration, Cartilage T2, Water content",
author = "Chou, {M. C.} and Tsai, {P. H.} and Huang, {G. S.} and Lee, {H. S.} and Lee, {C. H.} and Lin, {M. H.} and Lin, {C. Y.} and Chung, {H. W.}",
year = "2009",
month = "4",
doi = "10.1016/j.joca.2008.09.009",
language = "English",
volume = "17",
pages = "441--447",
journal = "Osteoarthritis and Cartilage",
issn = "1063-4584",
publisher = "W.B. Saunders Ltd",
number = "4",

}

TY - JOUR

T1 - Correlation between the MR T2 value at 4.7 T and relative water content in articular cartilage in experimental osteoarthritis induced by ACL transection

AU - Chou, M. C.

AU - Tsai, P. H.

AU - Huang, G. S.

AU - Lee, H. S.

AU - Lee, C. H.

AU - Lin, M. H.

AU - Lin, C. Y.

AU - Chung, H. W.

PY - 2009/4

Y1 - 2009/4

N2 - Objective: Both animal and human studies using magnetic resonance imaging (MRI) show that cartilage degeneration increases the T2 value. However, it is unclear whether the T2 value correlates linearly with water content in cartilage with osteoarthritis. The purpose of this study was to investigate the relationship between the T2 value and water content using an animal model of cartilage injury measured at 4.7 T. Design: Thirty Sprague Dawley rats were randomly separated into three groups (n = 10 for each group). Group 1 rats were not operated on (control). Group 2 rats received a sham operation, and group 3 rats received an anterior cruciate ligament (ACL) transection. Six rats of each group were randomly assigned to T2 measurement and later subjected to ex vivo analysis of the relative water content of the knee cartilage. The other four rats in each group were killed, and the severity of cartilage degeneration was examined histologically. The knees of the six rats in the ACL transection group were imaged sequentially 4 and 13 weeks after ACL transection, and the relative water content was measured at 13 weeks. Results: The cartilage T2 value was significantly higher 4 and 13 weeks after ACL transection in the operated knees than in the knees of the control and sham groups. The cartilage T2 value was significantly higher at 13 weeks than at 4 weeks in the operated knees. The T2 value was strongly positively correlated with the relative water content (R = 0.885, P <0.0001). Conclusion: The trend of changes in the T2 values is consistent with an increase in the relative water content in our cartilage degeneration model. This model has potential use for the clinical evaluation of osteoarthritis.

AB - Objective: Both animal and human studies using magnetic resonance imaging (MRI) show that cartilage degeneration increases the T2 value. However, it is unclear whether the T2 value correlates linearly with water content in cartilage with osteoarthritis. The purpose of this study was to investigate the relationship between the T2 value and water content using an animal model of cartilage injury measured at 4.7 T. Design: Thirty Sprague Dawley rats were randomly separated into three groups (n = 10 for each group). Group 1 rats were not operated on (control). Group 2 rats received a sham operation, and group 3 rats received an anterior cruciate ligament (ACL) transection. Six rats of each group were randomly assigned to T2 measurement and later subjected to ex vivo analysis of the relative water content of the knee cartilage. The other four rats in each group were killed, and the severity of cartilage degeneration was examined histologically. The knees of the six rats in the ACL transection group were imaged sequentially 4 and 13 weeks after ACL transection, and the relative water content was measured at 13 weeks. Results: The cartilage T2 value was significantly higher 4 and 13 weeks after ACL transection in the operated knees than in the knees of the control and sham groups. The cartilage T2 value was significantly higher at 13 weeks than at 4 weeks in the operated knees. The T2 value was strongly positively correlated with the relative water content (R = 0.885, P <0.0001). Conclusion: The trend of changes in the T2 values is consistent with an increase in the relative water content in our cartilage degeneration model. This model has potential use for the clinical evaluation of osteoarthritis.

KW - ACL transection

KW - Cartilage degeneration

KW - Cartilage T2

KW - Water content

UR - http://www.scopus.com/inward/record.url?scp=61549121095&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=61549121095&partnerID=8YFLogxK

U2 - 10.1016/j.joca.2008.09.009

DO - 10.1016/j.joca.2008.09.009

M3 - Article

VL - 17

SP - 441

EP - 447

JO - Osteoarthritis and Cartilage

JF - Osteoarthritis and Cartilage

SN - 1063-4584

IS - 4

ER -