Correlation between serum levels of anti-endothelial cell autoantigen and anti-dengue virus nonstructural protein 1 antibodies in dengue patients

Hsien Jen Cheng, Yueh Hsia Luo, Shu Wen Wan, Chiou Feng Lin, Shan Tair Wang, Nguyen Thanh Hung, Ching Chuan Liu, Tzong Shiann Ho, Hsiao Sheng Liu, Trai Ming Yeh, Yee Shin Lin

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

We have previously shown that anti-dengue virus nonstructural protein 1 (anti-DENV NS1) antibodies cross-react with endothelial cells, and several autoantigens have been identified. This study shows that the antibody levels against these self-proteins are higher in sera from patients with dengue hemorrhagic fever (DHF) than those in control sera. Anti-protein disulfide isomerase (PDI) and anti-heat shock protein 60 (anti-HSP60) IgM levels correlated with both anti-endothelial cells and anti-DENV NS1 IgM titers. A cross-reactive epitope on the NS1 amino acid residues 311-330 (P311-330) had been predicted. We further found that there were higher IgM and IgG levels against P311-330 in DHF patients' sera than those in the control sera. In addition, correlations were observed between anti-PDI with anti-P311-330 IgM and IgG levels, respectively. Therefore, our results indicate that DENV NS1 P311-330 is a major epitope for cross-reactive antibodies to PDI on the endothelial cell surface, which may play an important role in DENV infection-induced autoimmunity.

Original languageEnglish
Pages (from-to)989-995
Number of pages7
JournalAmerican Journal of Tropical Medicine and Hygiene
Volume92
Issue number5
DOIs
Publication statusPublished - May 1 2015

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ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases
  • Virology

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