Correlation between fluorescence in situ hybridization and testicular biopsy for the prediction of spermatogenesis in 37 patients with nonobstructive azoospermia

Han Sun Chiang, Shauh Der Yeh, Wei Min Lin, Chia Lang Fang, Hsiao Jui Wei

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Objectives. We applied interphase fluorescence in situ hybridization (FISH) to testis sections to examine the evidence of spermatogenesis in patients with nonobstructive azoospermia. This technique was evaluated and compared with conventional testicular histopathologic findings for the possibility of additional clinical applications. Methods. Thirty-seven consecutive patients with nonobstructive azoospermia were carefully evaluated clinically. Testes were biopsied for both sperm extraction and histopathologic examination. FISH staining was performed with a CEP 18 SpectrumAqua/CEP X SpectrumGreen/CEP Y SpectrumOrange probe. Results. Eight of 11 cases (sensitivity 73%) that were found to have spermatids on the histopathologic slides also were proven to produce haploid cells by FISH staining. On the other hand, 21 of the 26 cases (specificity 81%) for which no spermatids could be found on the histopathologic slides also had only diploid cells by FISH staining. On the basis of the good correlation between the FISH staining and conventional histopathologic findings, we could confirm the diagnosis of spermatogenesis using both methods. Conclusions. FISH staining of testicular sections allows more reliable prediction of spermatogenesis and provides benefits for a patient's decision regarding fertility counseling.

Original languageEnglish
Pages (from-to)1063-1068
Number of pages6
JournalUrology
Volume60
Issue number6
DOIs
Publication statusPublished - Dec 1 2002
Externally publishedYes

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Spermatogenesis
Fluorescence In Situ Hybridization
Biopsy
Staining and Labeling
Spermatids
Testis
Haploidy
Interphase
Diploidy
Fertility
Spermatozoa
Nonobstructive Azoospermia
Counseling

ASJC Scopus subject areas

  • Urology

Cite this

Correlation between fluorescence in situ hybridization and testicular biopsy for the prediction of spermatogenesis in 37 patients with nonobstructive azoospermia. / Chiang, Han Sun; Yeh, Shauh Der; Lin, Wei Min; Fang, Chia Lang; Wei, Hsiao Jui.

In: Urology, Vol. 60, No. 6, 01.12.2002, p. 1063-1068.

Research output: Contribution to journalArticle

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abstract = "Objectives. We applied interphase fluorescence in situ hybridization (FISH) to testis sections to examine the evidence of spermatogenesis in patients with nonobstructive azoospermia. This technique was evaluated and compared with conventional testicular histopathologic findings for the possibility of additional clinical applications. Methods. Thirty-seven consecutive patients with nonobstructive azoospermia were carefully evaluated clinically. Testes were biopsied for both sperm extraction and histopathologic examination. FISH staining was performed with a CEP 18 SpectrumAqua/CEP X SpectrumGreen/CEP Y SpectrumOrange probe. Results. Eight of 11 cases (sensitivity 73{\%}) that were found to have spermatids on the histopathologic slides also were proven to produce haploid cells by FISH staining. On the other hand, 21 of the 26 cases (specificity 81{\%}) for which no spermatids could be found on the histopathologic slides also had only diploid cells by FISH staining. On the basis of the good correlation between the FISH staining and conventional histopathologic findings, we could confirm the diagnosis of spermatogenesis using both methods. Conclusions. FISH staining of testicular sections allows more reliable prediction of spermatogenesis and provides benefits for a patient's decision regarding fertility counseling.",
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AU - Wei, Hsiao Jui

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