Copy number changes of CRISP3 in oral squamous cell carcinoma

Wen Chang Ko, Keisuke Sugahara, Takumi Sakuma, Ching Yu Yen, Shyun Yeu Liu, Gwo An Liaw, Takahiko Shibahara

Research output: Contribution to journalArticle

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Abstract

The aim of this study was to identify tumor suppressor genes (TSGs) in oral squamous cell carcinoma (OSCC) using whole-genome analysis of microarray technology and real-time quantitative polymerase chain reaction (QPCR). We applied whole-genome analysis of TSGs in the specimens from 3 patients of OSCC by microarray technology. A total of 11 genes, CRISP3, SCGB3A1, AGR2, PIP, C20orf114, TFF1, STATH, AZGP1, MUC7, DMBT1 and LOC389429, were found to be down-regulated, and 2, matrix metallopeptidase (MMP) 1 and MMP3, were found to be up-regulated in the 3 OSCC patients using microarray technology. In this study, we selected the CRISP3 gene. CRISP3 belongs to the cystein-rich secretary protein gene family in chromosome 6p12.3. CRISP3 has been found in the salivary gland, spleen and prostate gland and is a prominent biomarker in the gene expression of prostate cancer. Down-regulation of this gene was previously observed in OSCC. No studies examining the DNA copy number of CRISP3 in detail exist. We analyzed the DNA copy number of CRISP3 in 5 OSCC-derived cell lines (SAS, Ca9-22, KON, HSC2 and HSC4) and 60 OSCC tissues by real-time QPCR. The DNA copy number loss of CRISP3 was observed in 2 of the 5 OSCC-derived cell lines (SAS, HSC2) and in 24 of 60 patients (40.0%) using real-time QPCR. A significant statistical correlation between the copy number loss and gender and T classification was observed. These results indicate that the inactivation of CRISP3 is an early event in OSCC, since the T1/T2 classification is correlated with DNA copy number loss of CRISP3, whereas T3/T4 classification is not. We conclude that CRISP3 may be involved in the carcinogenesis of OSCC.

Original languageEnglish
Pages (from-to)75-81
Number of pages7
JournalOncology Letters
Volume3
Issue number1
DOIs
Publication statusPublished - Jan 2012

Fingerprint

Squamous Cell Carcinoma
Real-Time Polymerase Chain Reaction
DNA
Technology
Tumor Suppressor Genes
Genome
Genes
Cell Line
Microarray Analysis
Salivary Glands
Prostate
Prostatic Neoplasms
Carcinogenesis
Down-Regulation
Spleen
Chromosomes
Biomarkers
Gene Expression
Proteins

Keywords

  • DNA copy number
  • Microarray technology
  • Oral squamous cell carcinoma
  • Real-time quantitative polymerase chain reaction
  • Tumor suppressor gene

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Ko, W. C., Sugahara, K., Sakuma, T., Yen, C. Y., Liu, S. Y., Liaw, G. A., & Shibahara, T. (2012). Copy number changes of CRISP3 in oral squamous cell carcinoma. Oncology Letters, 3(1), 75-81. https://doi.org/10.3892/ol.2011.418

Copy number changes of CRISP3 in oral squamous cell carcinoma. / Ko, Wen Chang; Sugahara, Keisuke; Sakuma, Takumi; Yen, Ching Yu; Liu, Shyun Yeu; Liaw, Gwo An; Shibahara, Takahiko.

In: Oncology Letters, Vol. 3, No. 1, 01.2012, p. 75-81.

Research output: Contribution to journalArticle

Ko, WC, Sugahara, K, Sakuma, T, Yen, CY, Liu, SY, Liaw, GA & Shibahara, T 2012, 'Copy number changes of CRISP3 in oral squamous cell carcinoma', Oncology Letters, vol. 3, no. 1, pp. 75-81. https://doi.org/10.3892/ol.2011.418
Ko, Wen Chang ; Sugahara, Keisuke ; Sakuma, Takumi ; Yen, Ching Yu ; Liu, Shyun Yeu ; Liaw, Gwo An ; Shibahara, Takahiko. / Copy number changes of CRISP3 in oral squamous cell carcinoma. In: Oncology Letters. 2012 ; Vol. 3, No. 1. pp. 75-81.
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