Coordinate alteration of pp60(c-src) abundance and c-src RNA expression in human neuroblastoma variants

A. Veillette, J. O'Shaughnessy, I. D. Horak, M. A. Israel, D. Yee, N. Rosen, D. J. Fujita, H. J. Kung, J. L. Biedler, J. B. Bolen

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12 Citations (Scopus)

Abstract

We examined the expression, abundance, and protein kinase activity of pp60(c-src) in two different pairs of genetically indistinguishable cloned human neuroblastoma cell variants which display altered phenotypes as the result of conversion from a neuronal to a non-neuronal phenotype. The results demonstrate that cells which exhibit the neuroblastic (N-type) phenotype possess high levels of pp60(c-src) protein and that one of the two N-type cell lines is capable of expressing the neuronal-specific isoenzyme of pp60(c-src). In contrast, cells which display the substrate-adherent (S-type) phenotype have low levels of pp60(c-src) protein and express exclusively the non-neuronal isoenzyme of pp60(c-src). In all cells examined the abundance of pp60(c-src) was found to be proportional to the steady-state level of c-src RNA. In each case the protein kinase activity of pp60(c-src) was found to be proportional to the abundance of the protein and independent of the ratio of neuronal to non-neuronal species of pp60(c-src).

Original languageEnglish
Pages (from-to)421-427
Number of pages7
JournalOncogene
Volume4
Issue number4
Publication statusPublished - Jan 1 1989
Externally publishedYes

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cancer Research

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    Veillette, A., O'Shaughnessy, J., Horak, I. D., Israel, M. A., Yee, D., Rosen, N., Fujita, D. J., Kung, H. J., Biedler, J. L., & Bolen, J. B. (1989). Coordinate alteration of pp60(c-src) abundance and c-src RNA expression in human neuroblastoma variants. Oncogene, 4(4), 421-427.