Controlled surface morphology and hydrophilicity of polycaprolactone toward selective differentiation of mesenchymal stem cells to neural like cells

Hoda Jahani, Farid Azizi Jalilian, Chia Yu Wu, Saeid Kaviani, Masoud Soleimani, Naghmeh Abassi, Keng Liang Ou, Hossein Hosseinkhani

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Differentiation of mesenchymal stem cells (MSCs) into neuron cells has great potential in therapy of damaged nerve tissue. It has been shown that three-dimensional biomaterials have great ability to up regulate the expression of neuronal proteins. In this study, O2 plasma technology was used to enhance hydrophilicity of poly (ε-caprolactone) (PCL) toward selective differentiation of MSCs into neural cells. Random and aligned PCL nanofibers scaffolds were fabricated by electrospinning method and their physicochemical and mechanical properties were carried out by scanning electron microscope (SEM), contact angle, and tensile measurements. Contact angle studies of PCL and plasma treated PCL (p-PCL) nanofibers revealed significant change on the surface properties PCL nanofibers from the view point of hydrophilicity. Physiochemical studies revealed that p-PCL nanofibers were extremely hydrophilic compared with untreated PCL nanofibers which were highly hydrophobic and nonabsorbent to water. Differentiation of MSCs were carried out by inducing growth factors including basic fibroblast growth factor, nerve growth factor, and brain derived growth factor, NT3, 3-isobutyl-1-methylxanthine (IBMX) in Dulbecco's modified Eagle's medium/F12 media. Differentiated MSCs on nanofibrous scaffold were examined by immunofluorescence assay and was found to express the neuronal proteins; β-tubulin III and Map2, on day 15 after cell culture. The real-time polymerase chain reaction (RT-PCR) analysis showed that p-PCL nanofibrous scaffold could upregulate expression of Map-2 and downregulate expression of Nestin genes in nerve cells differentiated from MSCs. This study indicates that mesenchymal stem cell cultured on nanofibrous scaffold have potential differentiation to neuronal cells on and could apply in nerve tissue repair.

Original languageEnglish
Pages (from-to)1875-1881
Number of pages7
JournalJournal of Biomedical Materials Research - Part A
Volume103
Issue number5
DOIs
Publication statusPublished - May 1 2015

Fingerprint

Polycaprolactone
Hydrophilicity
Stem cells
Surface morphology
Nanofibers
Scaffolds
Plasmas
Neurons
Contact angle
Tissue
Proteins
1-Methyl-3-isobutylxanthine
Nestin
Polymerase chain reaction
Electrospinning
Biocompatible Materials
Nerve Growth Factor
Fibroblast Growth Factor 2
Fibroblasts
Tubulin

Keywords

  • hydrophilicity
  • mesenchymal stem cells
  • neural cells
  • surface modification
  • tissue engineering

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials
  • Ceramics and Composites
  • Metals and Alloys

Cite this

Controlled surface morphology and hydrophilicity of polycaprolactone toward selective differentiation of mesenchymal stem cells to neural like cells. / Jahani, Hoda; Jalilian, Farid Azizi; Wu, Chia Yu; Kaviani, Saeid; Soleimani, Masoud; Abassi, Naghmeh; Ou, Keng Liang; Hosseinkhani, Hossein.

In: Journal of Biomedical Materials Research - Part A, Vol. 103, No. 5, 01.05.2015, p. 1875-1881.

Research output: Contribution to journalArticle

Jahani, Hoda ; Jalilian, Farid Azizi ; Wu, Chia Yu ; Kaviani, Saeid ; Soleimani, Masoud ; Abassi, Naghmeh ; Ou, Keng Liang ; Hosseinkhani, Hossein. / Controlled surface morphology and hydrophilicity of polycaprolactone toward selective differentiation of mesenchymal stem cells to neural like cells. In: Journal of Biomedical Materials Research - Part A. 2015 ; Vol. 103, No. 5. pp. 1875-1881.
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