Contribution of HDL-apolipoproteins to the inhibition of low density lipoprotein oxidation and lipid accumulation in macrophages

Kae Yuan Lin, Yuh Lien Chen, Chun Che Shih, Ju Pin Pan, Woan Eng Chan, An Na Chiang

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

High-density lipoprotein (HDL) is known as a protective factor against atherosclerosis. However, whether HDL-apolipoproteins (apo-HDL) contribute to the protection in arterial cells remains unclear. The localization patterns of human apolipoproteins in atherosclerotic arteries were determined using immunohistochemical examination. The results indicate that several apolipoproteins are retained in component cells of the coronary artery walls. To elucidate the possible roles of apo-HDL in the protection of atherosclerotic lesion formation, we investigated the effects of apo-HDL on the formation of conjugated diene (CD) in a cell-free system and thiobarbituric acid-reactive substances (TBARS) in the medium of a macrophage-mediated LDL oxidation system. The results showed that apo-HDL significantly exerted an inhibitory effect on LDL lipid oxidation in vitro. In addition, apo-HDL decreased cholesterol influx but enhanced cholesterol efflux from J774 macrophages in a dose-dependent manner. These results are consistent with the notion that there is reduced intracellular lipid accumulation in apo-HDL treated macrophages. These data provide a direct evidence for apo-HDL in protecting LDL from oxidative modification and in reducing the accumulation of cholesterol and lipid droplets by J774 macrophages.

Original languageEnglish
Pages (from-to)258-267
Number of pages10
JournalJournal of Cellular Biochemistry
Volume86
Issue number2
DOIs
Publication statusPublished - Jul 18 2002
Externally publishedYes

Keywords

  • Atherosclerosis
  • Cholesterol efflux
  • Cholesterol influx
  • Lipid accumulation
  • Oxidative modification

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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