Continuous cell line from pupal ovary of Perina nuda (Lepidoptera: Lymantriidae) that is permissive to nuclear polyhedrosis virus from P. nuda

Chung Hsiung Wang, Chih Ming Chou, Hwei Chung Liu, Shih Liang Kau, Guang Hsung Kou, Chu Fang Lo

Research output: Contribution to journalArticlepeer-review

21 Citations (Scopus)

Abstract

A continuous cell line, designated NTU-PN-HH, was established from the pupal ovary of Perina nuda Fabricius (Lepidoptera: Lymantriidae). The cells have been through more than 300 passages during 4 years in TNM-FH medium supplemented with 10% FBS, at a constant temperature of 28°C. The cell line consists of four major morphologic types: polymorphic cells, spindle-shaped cells, round cells, and squamous cells. The characterization of this cell line showed that NTU-PN-HH is a newly established cell line. It is the first cell line that is permissive to PnNPV (P. nuda multiple nuclear polyhedrosis virus).

Original languageEnglish
Pages (from-to)199-204
Number of pages6
JournalJournal of Invertebrate Pathology
Volume67
Issue number3
DOIs
Publication statusPublished - May 1996
Externally publishedYes

Keywords

  • NTU-PN-HH cell line
  • Perina nuda
  • PnNPV

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Insect Science

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