Construction and characterization of a novel fusion protein consisting of anti-CD3 antibody fused to recombinant interleukin-2

Kuan Der Lee, Hsin Wei Chen, Chih G. Chen, Yan Chung Shih, Hsing Kun Liu, Mei Lien Cheng

Research output: Contribution to journalArticlepeer-review

Abstract

T cells can be activated in vitro by monoclonal antibodies to CD3 (anti-CD3) to become non-MHC restricted killer cells (CD3-AK). Anti-CD3 activation upregulates the expression of the interleukin (IL)-2 receptors on T cells whose expansion is facilitated by IL-2. The therapeutic effect of in vivo administration of anti-CD3 and IL-2 has been investigated in many types of human cancers. To circumvent the toxicities posed by systemic administration of high-dose IL-2, there is interest in forming a strategy for targeting and concentrating IL-2 at the site where it is needed. This study investigates the feasibility of constructing a novel fusion protein consisting of IL-2 fused to the constant region of anti-CD3 antibody. Our results indicate that the specific IL-2 receptor-binding capability and bioactivity of the IL-2 portion as well as the CD3-binding and biological functions of anti-CD3 portion remain intact in this anti-CD3/IL-2 fusion protein. Thus, cytokines fused to anti-CD3 antibody by genetic engineering is feasible and may provide a new class of immunotherapeutics for cancer.

Original languageEnglish
Pages (from-to)1211-1216
Number of pages6
JournalOncology Reports
Volume15
Issue number5
Publication statusPublished - May 2006
Externally publishedYes

Keywords

  • Anti-CD3
  • Fusion protein
  • IL-2

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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