Conjugated linoleic acids suppress inflammatory response and ICAM-1 expression through inhibition of NF-κB and MAPK signaling in human bronchial epithelial cells

Wen Chung Huang, Rong Syuan Tu, Ya Ling Chen, Yun Yun Tsai, Chwan Fwu Lin, Chian Jiun Liou

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Conjugated linoleic acids (CLAs) comprise a group of natural unsaturated fatty acids. CLA was reported to have anti-asthma, anti-adiposity, and anti-tumor effects. The present study aimed to evaluate the suppressive effects of cis-9, trans-11-CLA (c9,t11-CLA) on the expression of proinflammatory cytokines and intercellular adhesion molecule 1 (ICAM-1) in TNF-α-stimulated human bronchial epithelial (BEAS-2B) cells. After treating with various doses of c9,t11-CLA (12.5-100 μg ml-1), BEAS-2B cells were induced into an inflamed state by adding TNF-α or TNF-α/IL-4. The presence of c9,t11-CLA significantly suppressed the secretion of cytokines IL-6, IL-8, CCL5, and MCP-1. We also found that c9,t11-CLA inhibited ICAM-1 expression, and decreased monocyte adhesion to inflamed bronchial epithelial cells. Interestingly, c9,t11-CLA attenuated the phosphorylation of mitogen-activated protein kinase (MAPK) and down-regulated the activation of nuclear factor-κB (NF-κB). These results suggested that the anti-inflammatory effects of c9,t11-CLA were mediated by inhibiting proinflammatory cytokines, chemokines, and ICAM-1 expression by blocking NF-κB transcription regulation and by attenuating MAPK signaling pathways.

Original languageEnglish
Pages (from-to)2025-2033
Number of pages9
JournalFood and Function
Volume7
Issue number4
DOIs
Publication statusPublished - Apr 1 2016
Externally publishedYes

Fingerprint

Conjugated Linoleic Acids
Intercellular Adhesion Molecule-1
conjugated linoleic acid
Mitogen-Activated Protein Kinases
mitogen-activated protein kinase
adhesion
epithelial cells
inflammation
Epithelial Cells
Cytokines
cytokines
Adiposity
Unsaturated Fatty Acids
Interleukin-8
Chemokines
Interleukin-4
Monocytes
Interleukin-6
interleukin-8
1-methylcyclopropene

ASJC Scopus subject areas

  • Food Science

Cite this

Conjugated linoleic acids suppress inflammatory response and ICAM-1 expression through inhibition of NF-κB and MAPK signaling in human bronchial epithelial cells. / Huang, Wen Chung; Tu, Rong Syuan; Chen, Ya Ling; Tsai, Yun Yun; Lin, Chwan Fwu; Liou, Chian Jiun.

In: Food and Function, Vol. 7, No. 4, 01.04.2016, p. 2025-2033.

Research output: Contribution to journalArticle

Huang, Wen Chung ; Tu, Rong Syuan ; Chen, Ya Ling ; Tsai, Yun Yun ; Lin, Chwan Fwu ; Liou, Chian Jiun. / Conjugated linoleic acids suppress inflammatory response and ICAM-1 expression through inhibition of NF-κB and MAPK signaling in human bronchial epithelial cells. In: Food and Function. 2016 ; Vol. 7, No. 4. pp. 2025-2033.
@article{f74580bd23494570b57a225c2cadf283,
title = "Conjugated linoleic acids suppress inflammatory response and ICAM-1 expression through inhibition of NF-κB and MAPK signaling in human bronchial epithelial cells",
abstract = "Conjugated linoleic acids (CLAs) comprise a group of natural unsaturated fatty acids. CLA was reported to have anti-asthma, anti-adiposity, and anti-tumor effects. The present study aimed to evaluate the suppressive effects of cis-9, trans-11-CLA (c9,t11-CLA) on the expression of proinflammatory cytokines and intercellular adhesion molecule 1 (ICAM-1) in TNF-α-stimulated human bronchial epithelial (BEAS-2B) cells. After treating with various doses of c9,t11-CLA (12.5-100 μg ml-1), BEAS-2B cells were induced into an inflamed state by adding TNF-α or TNF-α/IL-4. The presence of c9,t11-CLA significantly suppressed the secretion of cytokines IL-6, IL-8, CCL5, and MCP-1. We also found that c9,t11-CLA inhibited ICAM-1 expression, and decreased monocyte adhesion to inflamed bronchial epithelial cells. Interestingly, c9,t11-CLA attenuated the phosphorylation of mitogen-activated protein kinase (MAPK) and down-regulated the activation of nuclear factor-κB (NF-κB). These results suggested that the anti-inflammatory effects of c9,t11-CLA were mediated by inhibiting proinflammatory cytokines, chemokines, and ICAM-1 expression by blocking NF-κB transcription regulation and by attenuating MAPK signaling pathways.",
author = "Huang, {Wen Chung} and Tu, {Rong Syuan} and Chen, {Ya Ling} and Tsai, {Yun Yun} and Lin, {Chwan Fwu} and Liou, {Chian Jiun}",
year = "2016",
month = "4",
day = "1",
doi = "10.1039/c5fo01037c",
language = "English",
volume = "7",
pages = "2025--2033",
journal = "Food and Function",
issn = "2042-6496",
publisher = "Royal Society of Chemistry",
number = "4",

}

TY - JOUR

T1 - Conjugated linoleic acids suppress inflammatory response and ICAM-1 expression through inhibition of NF-κB and MAPK signaling in human bronchial epithelial cells

AU - Huang, Wen Chung

AU - Tu, Rong Syuan

AU - Chen, Ya Ling

AU - Tsai, Yun Yun

AU - Lin, Chwan Fwu

AU - Liou, Chian Jiun

PY - 2016/4/1

Y1 - 2016/4/1

N2 - Conjugated linoleic acids (CLAs) comprise a group of natural unsaturated fatty acids. CLA was reported to have anti-asthma, anti-adiposity, and anti-tumor effects. The present study aimed to evaluate the suppressive effects of cis-9, trans-11-CLA (c9,t11-CLA) on the expression of proinflammatory cytokines and intercellular adhesion molecule 1 (ICAM-1) in TNF-α-stimulated human bronchial epithelial (BEAS-2B) cells. After treating with various doses of c9,t11-CLA (12.5-100 μg ml-1), BEAS-2B cells were induced into an inflamed state by adding TNF-α or TNF-α/IL-4. The presence of c9,t11-CLA significantly suppressed the secretion of cytokines IL-6, IL-8, CCL5, and MCP-1. We also found that c9,t11-CLA inhibited ICAM-1 expression, and decreased monocyte adhesion to inflamed bronchial epithelial cells. Interestingly, c9,t11-CLA attenuated the phosphorylation of mitogen-activated protein kinase (MAPK) and down-regulated the activation of nuclear factor-κB (NF-κB). These results suggested that the anti-inflammatory effects of c9,t11-CLA were mediated by inhibiting proinflammatory cytokines, chemokines, and ICAM-1 expression by blocking NF-κB transcription regulation and by attenuating MAPK signaling pathways.

AB - Conjugated linoleic acids (CLAs) comprise a group of natural unsaturated fatty acids. CLA was reported to have anti-asthma, anti-adiposity, and anti-tumor effects. The present study aimed to evaluate the suppressive effects of cis-9, trans-11-CLA (c9,t11-CLA) on the expression of proinflammatory cytokines and intercellular adhesion molecule 1 (ICAM-1) in TNF-α-stimulated human bronchial epithelial (BEAS-2B) cells. After treating with various doses of c9,t11-CLA (12.5-100 μg ml-1), BEAS-2B cells were induced into an inflamed state by adding TNF-α or TNF-α/IL-4. The presence of c9,t11-CLA significantly suppressed the secretion of cytokines IL-6, IL-8, CCL5, and MCP-1. We also found that c9,t11-CLA inhibited ICAM-1 expression, and decreased monocyte adhesion to inflamed bronchial epithelial cells. Interestingly, c9,t11-CLA attenuated the phosphorylation of mitogen-activated protein kinase (MAPK) and down-regulated the activation of nuclear factor-κB (NF-κB). These results suggested that the anti-inflammatory effects of c9,t11-CLA were mediated by inhibiting proinflammatory cytokines, chemokines, and ICAM-1 expression by blocking NF-κB transcription regulation and by attenuating MAPK signaling pathways.

UR - http://www.scopus.com/inward/record.url?scp=84966389243&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84966389243&partnerID=8YFLogxK

U2 - 10.1039/c5fo01037c

DO - 10.1039/c5fo01037c

M3 - Article

C2 - 27007063

AN - SCOPUS:84966389243

VL - 7

SP - 2025

EP - 2033

JO - Food and Function

JF - Food and Function

SN - 2042-6496

IS - 4

ER -