Conformational and adsorptive characteristics of albumin affect interfacial protein boundary lubrication: From experimental to molecular dynamics simulation approaches

Hsu Wei Fang, Man Ching Hsieh, Huei Ting Huang, Cheng Yen Tsai, Min Hui Chang

Research output: Contribution to journalArticle

27 Citations (Scopus)


The lifetime of artificial joints is mainly determined by their biotribological properties. Synovial fluid which consists of various biological molecules acts as the lubricant. Among the compositions of synovial fluid, albumin is the most abundant protein. Under high load and low sliding speed articulation of artificial joint, it is believed the lubricants form protective layers on the sliding surfaces under the boundary lubrication mechanism. The protective molecular layer keeps two surfaces from direct collision and thus decreases the possibility of wear damage. However, the lubricating ability of the molecular layer may vary due to the conformational change of albumin in the process. In this study, we investigated the influence of albumin conformation on the adsorption behaviors on the articulating surfaces and discuss the relationship between adsorbed albumin and its tribological behaviors. We performed the friction tests to study the effects of albumin unfolding on the frictional behaviors. The novelty of this research is to further carry out molecular dynamics simulation, and protein adsorption experiments to investigate the mechanisms of the albumin-mediated boundary lubrication of arthroplastic materials. It was observed that the thermal processes induce the loss of secondary structure of albumin. The compactness of the unfolded structure leads to a higher adsorption rate onto the articulating material surface and results in the increase of friction coefficient.

Original languageEnglish
Pages (from-to)171-177
Number of pages7
JournalColloids and Surfaces B: Biointerfaces
Issue number2
Publication statusPublished - Feb 1 2009
Externally publishedYes



  • Adsorption
  • Albumin
  • Boundary lubrication
  • Conformation
  • Friction
  • Molecular dynamics

ASJC Scopus subject areas

  • Biotechnology
  • Surfaces and Interfaces
  • Physical and Theoretical Chemistry
  • Colloid and Surface Chemistry

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