Confocal laser scanning microscopy

I. An overview of principle and practice in biomedical research

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Evolving from conventional microscopic technologies, confocal microscopy has proved itself to play an important role in the biomedical research during the past decade. Confocal microscope has many advantages over traditional microscope including the ability to look deeply into inside cells with less photodamage and photobleach, reconstruct three-dimensional images, and chart intracellular dynamic events in the living cells. With these remarkable properties and the availability of fluorescent dyes for living cells, the confocal microscopy has been widely used in solving many unknown questions in biological and pharmacological fields. In clinics, confocal microscope has also served as an important tool to observe the living cells in skins and eyes. For anesthesiologists, confocal microscope has made possible novel experimental approaches for the effects of multiple anesthetic agents on cells. Furthermore, the technology of fibreoptical confocal endomicroscopy is now on its way of maturation. It will soon be the era for confocal microscopy to explore the "cell behavior" inside of intact living tissues.

Original languageEnglish
Pages (from-to)33-40
Number of pages8
JournalActa Anaesthesiologica Taiwanica
Volume42
Issue number1
Publication statusPublished - Mar 2004

Fingerprint

Confocal Microscopy
Biomedical Research
Technology
Three-Dimensional Imaging
Fluorescent Dyes
Anesthetics
Pharmacology
Skin

Keywords

  • Confocal
  • Microscopy

ASJC Scopus subject areas

  • Anesthesiology and Pain Medicine

Cite this

@article{8b6ccfa850fe41f3bdb37d145a155922,
title = "Confocal laser scanning microscopy: I. An overview of principle and practice in biomedical research",
abstract = "Evolving from conventional microscopic technologies, confocal microscopy has proved itself to play an important role in the biomedical research during the past decade. Confocal microscope has many advantages over traditional microscope including the ability to look deeply into inside cells with less photodamage and photobleach, reconstruct three-dimensional images, and chart intracellular dynamic events in the living cells. With these remarkable properties and the availability of fluorescent dyes for living cells, the confocal microscopy has been widely used in solving many unknown questions in biological and pharmacological fields. In clinics, confocal microscope has also served as an important tool to observe the living cells in skins and eyes. For anesthesiologists, confocal microscope has made possible novel experimental approaches for the effects of multiple anesthetic agents on cells. Furthermore, the technology of fibreoptical confocal endomicroscopy is now on its way of maturation. It will soon be the era for confocal microscopy to explore the {"}cell behavior{"} inside of intact living tissues.",
keywords = "Confocal, Microscopy",
author = "Chen, {Jui Tai} and Ruei-Ming Chen and Lin, {Yi Ling} and Huai-Chia Chang and Lin, {Yu Hua} and Ta-Liang Chen and Tyng-Guey Chen",
year = "2004",
month = "3",
language = "English",
volume = "42",
pages = "33--40",
journal = "Asian Journal of Anesthesiology",
issn = "2468-824X",
publisher = "Elsevier Taiwan LLC",
number = "1",

}

TY - JOUR

T1 - Confocal laser scanning microscopy

T2 - I. An overview of principle and practice in biomedical research

AU - Chen, Jui Tai

AU - Chen, Ruei-Ming

AU - Lin, Yi Ling

AU - Chang, Huai-Chia

AU - Lin, Yu Hua

AU - Chen, Ta-Liang

AU - Chen, Tyng-Guey

PY - 2004/3

Y1 - 2004/3

N2 - Evolving from conventional microscopic technologies, confocal microscopy has proved itself to play an important role in the biomedical research during the past decade. Confocal microscope has many advantages over traditional microscope including the ability to look deeply into inside cells with less photodamage and photobleach, reconstruct three-dimensional images, and chart intracellular dynamic events in the living cells. With these remarkable properties and the availability of fluorescent dyes for living cells, the confocal microscopy has been widely used in solving many unknown questions in biological and pharmacological fields. In clinics, confocal microscope has also served as an important tool to observe the living cells in skins and eyes. For anesthesiologists, confocal microscope has made possible novel experimental approaches for the effects of multiple anesthetic agents on cells. Furthermore, the technology of fibreoptical confocal endomicroscopy is now on its way of maturation. It will soon be the era for confocal microscopy to explore the "cell behavior" inside of intact living tissues.

AB - Evolving from conventional microscopic technologies, confocal microscopy has proved itself to play an important role in the biomedical research during the past decade. Confocal microscope has many advantages over traditional microscope including the ability to look deeply into inside cells with less photodamage and photobleach, reconstruct three-dimensional images, and chart intracellular dynamic events in the living cells. With these remarkable properties and the availability of fluorescent dyes for living cells, the confocal microscopy has been widely used in solving many unknown questions in biological and pharmacological fields. In clinics, confocal microscope has also served as an important tool to observe the living cells in skins and eyes. For anesthesiologists, confocal microscope has made possible novel experimental approaches for the effects of multiple anesthetic agents on cells. Furthermore, the technology of fibreoptical confocal endomicroscopy is now on its way of maturation. It will soon be the era for confocal microscopy to explore the "cell behavior" inside of intact living tissues.

KW - Confocal

KW - Microscopy

UR - http://www.scopus.com/inward/record.url?scp=2942694442&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=2942694442&partnerID=8YFLogxK

M3 - Article

VL - 42

SP - 33

EP - 40

JO - Asian Journal of Anesthesiology

JF - Asian Journal of Anesthesiology

SN - 2468-824X

IS - 1

ER -