Composition of polyphenols in fresh tea leaves and associations of their oxygen-radical-absorbing capacity with antiproliferative actions in fibroblast cells

Yu Li Lin, I. Ming Juan, Ying Ling Chen, Yu Chih Liang, Jen Kun Lin

Research output: Contribution to journalArticle

226 Citations (Scopus)

Abstract

The polyphenols in various parts of the tea plant used in manufacture of tea products (young leaves, old leaves, and stem) were analyzed by HPLC. The young leaves (apical bud and the two youngest leaves) were found to be richer (2.7-fold) in polyphenols than old leaves (from the tenth to the fifth leaf). Also, the tea polyphenols were found to be higher (1.4-fold) in summer than in spring. Ten different types of commercial tea (manufactured tea), including unfermented, semifermented, and fermented tea, were analyzed for their polyphenol compounds, and it was found that both yields of solids in tea water extracts (TWEs) and the amount of (-)-epigallocatechin 3-gallate (EGCG) in these products varied with different tea leaves and processing methods. Longjing tea (unfermented green tea) contained the highest concentration of EGCG and polyphenols, whereas Assam black tea (most fermented) contained the least. Longjing TWEs showed strong inhibitory effect on DNA synthesis in A-431 tumor cells. We also found that green tea polyphenols (GTPs) and EGCG strongly inhibited tumor cell DNA synthesis and peroxyl-radical generation. The evidence suggests that the strong antioxidative and antiproliferative activities of Longjing TWEs and GTPs are mainly due to the higher contents of tea polyphenols. EGCG (the major component of GTPs) showed strong inhibitory action on the growth of immortalized cells (NIH3T3) and tumor cells (S-180II) but almost no effect on the growth of normal cells (C3H10T1/2).

Original languageEnglish
Pages (from-to)1387-1394
Number of pages8
JournalJournal of Agricultural and Food Chemistry
Volume44
Issue number6
Publication statusPublished - Jun 1996
Externally publishedYes

Fingerprint

tea (beverage)
Polyphenols
Tea
Fibroblasts
fibroblasts
reactive oxygen species
Reactive Oxygen Species
polyphenols
Cells
Chemical analysis
leaves
epigallocatechin
green tea
Tumors
tea
extracts
Water
synthesis
black tea
water

Keywords

  • Antioxidative effect
  • Antiproliferative effect
  • EGCG
  • Polyphenols
  • Tea water extracts

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Food Science
  • Chemistry (miscellaneous)

Cite this

Composition of polyphenols in fresh tea leaves and associations of their oxygen-radical-absorbing capacity with antiproliferative actions in fibroblast cells. / Lin, Yu Li; Juan, I. Ming; Chen, Ying Ling; Liang, Yu Chih; Lin, Jen Kun.

In: Journal of Agricultural and Food Chemistry, Vol. 44, No. 6, 06.1996, p. 1387-1394.

Research output: Contribution to journalArticle

@article{5855c37d292f45a0b5c0089ed3f3bc0b,
title = "Composition of polyphenols in fresh tea leaves and associations of their oxygen-radical-absorbing capacity with antiproliferative actions in fibroblast cells",
abstract = "The polyphenols in various parts of the tea plant used in manufacture of tea products (young leaves, old leaves, and stem) were analyzed by HPLC. The young leaves (apical bud and the two youngest leaves) were found to be richer (2.7-fold) in polyphenols than old leaves (from the tenth to the fifth leaf). Also, the tea polyphenols were found to be higher (1.4-fold) in summer than in spring. Ten different types of commercial tea (manufactured tea), including unfermented, semifermented, and fermented tea, were analyzed for their polyphenol compounds, and it was found that both yields of solids in tea water extracts (TWEs) and the amount of (-)-epigallocatechin 3-gallate (EGCG) in these products varied with different tea leaves and processing methods. Longjing tea (unfermented green tea) contained the highest concentration of EGCG and polyphenols, whereas Assam black tea (most fermented) contained the least. Longjing TWEs showed strong inhibitory effect on DNA synthesis in A-431 tumor cells. We also found that green tea polyphenols (GTPs) and EGCG strongly inhibited tumor cell DNA synthesis and peroxyl-radical generation. The evidence suggests that the strong antioxidative and antiproliferative activities of Longjing TWEs and GTPs are mainly due to the higher contents of tea polyphenols. EGCG (the major component of GTPs) showed strong inhibitory action on the growth of immortalized cells (NIH3T3) and tumor cells (S-180II) but almost no effect on the growth of normal cells (C3H10T1/2).",
keywords = "Antioxidative effect, Antiproliferative effect, EGCG, Polyphenols, Tea water extracts",
author = "Lin, {Yu Li} and Juan, {I. Ming} and Chen, {Ying Ling} and Liang, {Yu Chih} and Lin, {Jen Kun}",
year = "1996",
month = "6",
language = "English",
volume = "44",
pages = "1387--1394",
journal = "Journal of Agricultural and Food Chemistry",
issn = "0021-8561",
publisher = "American Chemical Society",
number = "6",

}

TY - JOUR

T1 - Composition of polyphenols in fresh tea leaves and associations of their oxygen-radical-absorbing capacity with antiproliferative actions in fibroblast cells

AU - Lin, Yu Li

AU - Juan, I. Ming

AU - Chen, Ying Ling

AU - Liang, Yu Chih

AU - Lin, Jen Kun

PY - 1996/6

Y1 - 1996/6

N2 - The polyphenols in various parts of the tea plant used in manufacture of tea products (young leaves, old leaves, and stem) were analyzed by HPLC. The young leaves (apical bud and the two youngest leaves) were found to be richer (2.7-fold) in polyphenols than old leaves (from the tenth to the fifth leaf). Also, the tea polyphenols were found to be higher (1.4-fold) in summer than in spring. Ten different types of commercial tea (manufactured tea), including unfermented, semifermented, and fermented tea, were analyzed for their polyphenol compounds, and it was found that both yields of solids in tea water extracts (TWEs) and the amount of (-)-epigallocatechin 3-gallate (EGCG) in these products varied with different tea leaves and processing methods. Longjing tea (unfermented green tea) contained the highest concentration of EGCG and polyphenols, whereas Assam black tea (most fermented) contained the least. Longjing TWEs showed strong inhibitory effect on DNA synthesis in A-431 tumor cells. We also found that green tea polyphenols (GTPs) and EGCG strongly inhibited tumor cell DNA synthesis and peroxyl-radical generation. The evidence suggests that the strong antioxidative and antiproliferative activities of Longjing TWEs and GTPs are mainly due to the higher contents of tea polyphenols. EGCG (the major component of GTPs) showed strong inhibitory action on the growth of immortalized cells (NIH3T3) and tumor cells (S-180II) but almost no effect on the growth of normal cells (C3H10T1/2).

AB - The polyphenols in various parts of the tea plant used in manufacture of tea products (young leaves, old leaves, and stem) were analyzed by HPLC. The young leaves (apical bud and the two youngest leaves) were found to be richer (2.7-fold) in polyphenols than old leaves (from the tenth to the fifth leaf). Also, the tea polyphenols were found to be higher (1.4-fold) in summer than in spring. Ten different types of commercial tea (manufactured tea), including unfermented, semifermented, and fermented tea, were analyzed for their polyphenol compounds, and it was found that both yields of solids in tea water extracts (TWEs) and the amount of (-)-epigallocatechin 3-gallate (EGCG) in these products varied with different tea leaves and processing methods. Longjing tea (unfermented green tea) contained the highest concentration of EGCG and polyphenols, whereas Assam black tea (most fermented) contained the least. Longjing TWEs showed strong inhibitory effect on DNA synthesis in A-431 tumor cells. We also found that green tea polyphenols (GTPs) and EGCG strongly inhibited tumor cell DNA synthesis and peroxyl-radical generation. The evidence suggests that the strong antioxidative and antiproliferative activities of Longjing TWEs and GTPs are mainly due to the higher contents of tea polyphenols. EGCG (the major component of GTPs) showed strong inhibitory action on the growth of immortalized cells (NIH3T3) and tumor cells (S-180II) but almost no effect on the growth of normal cells (C3H10T1/2).

KW - Antioxidative effect

KW - Antiproliferative effect

KW - EGCG

KW - Polyphenols

KW - Tea water extracts

UR - http://www.scopus.com/inward/record.url?scp=0001619794&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0001619794&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:0001619794

VL - 44

SP - 1387

EP - 1394

JO - Journal of Agricultural and Food Chemistry

JF - Journal of Agricultural and Food Chemistry

SN - 0021-8561

IS - 6

ER -